Evaluation of a New Recombinant Antigen-Based Virotech Treponema Pallidum Screen ELISA for Diagnosis of Syphilis

Busse, Clemens; Navid, Mojdeh Heidary; Strubel, Andreas; Schnitzler, Paul

doi:10.7754/clin.lab.2012.120519

Cited by 11 publications

(7 citation statements)

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“…Serological tests for syphilis (STS) 14,[25][26][27][28][29][30][31][32][33][34][35][36][37] STS provide a presumptive diagnosis of syphilis. None of the STS differentiate between venereal syphilis and the non-venereal treponematoses (yaws: T. pallidum subsp pertenue; bejelendemic syphilis: T. pallidum subsp endemicum and pinta: T. carateum).…”

Section: Demonstration Of T Pallidummentioning

confidence: 99%

2014 European guideline on the management of syphilis

Janier

Hegyi

Dupin

et al. 2014

Acad Dermatol Venereol

350

348

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Section: Demonstration Of T Pallidummentioning

confidence: 99%

2014 European guideline on the management of syphilis

Janier

Hegyi

Dupin

et al. 2014

Acad Dermatol Venereol

350

348

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“…Odczyny serologiczne w kile [14,[25][26][27][28][29][30][31][32][33][34][35][36][37] Odczyny serologiczne umożliwiają prawdopodobne rozpoznanie kiły.…”

Section: Diagnostyka Laboratoryjnaunclassified

2014 European guideline on the management of syphilis

Janier¹,

Hegyi²,

Dupin³

et al. 2015

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“…At present, common laboratory detection methods include direct pathogen detection, serological assessment and polymerase chain reaction (PCR) analysis (6). In particular, serological assessment is commonly used in the clinical screening and diagnosis of syphilis, and primarily includes non-Tp and Tp antigen serum tests (7). The non-Tp antigen serum assessment utilizes non-specific lipoids as antigens and detects blood reagin as a syphilitic marker (8).…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity and immunoreactivity of Tp0821 recombinant protein from Treponema pallidum

et al. 2017

Molecular Medicine Reports

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Treponema pallidum (Tp) is responsible for invading reproductive organs and the skin in early stages, and involves almost all organs/systems at advanced stages. In the present study, screening of the dominant epitope fragment of the Tp outer membrane protein, Tp0821, was performed and the prokaryotic expression vector pQE32/Tp0821 was constructed. The denaturation and dialysis of rTp0821 were achieved through ultrasound, inclusion body washing and dissolution. Experiments in purified rTp0821‑immune New Zealand rabbits indicated that the recombinant proteins were of high immunogenicity, and the irritation led a marked humoral immune response in the New Zealand rabbits. Western blot analysis showed that the purified recombinant proteins reacted with the Tp‑positive infected serum, confirming the high level of immunoreactivity. The delayed type hypersensitivity of rTp0821 recombinant proteins was positive, indicating that rTp0821 induced a specific cell immune response and was selected as a Tp vaccine candidate protein. The findings of the present study provided novel evidence, which provided information for further investigations on the pathogenic mechanism of Tp and the development of diagnostic reagents.

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Evaluation of a New Recombinant Antigen-Based Virotech Treponema Pallidum Screen ELISA for Diagnosis of Syphilis

Cited by 11 publications

References 0 publications

2014 European guideline on the management of syphilis

2014 European guideline on the management of syphilis

2014 European guideline on the management of syphilis

Immunogenicity and immunoreactivity of Tp0821 recombinant protein from Treponema pallidum

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