Evaluation of a New Dot Blot Enzyme Immunoassay (Directigen Flu A+B) for Simultaneous and Differential Detection of Influenza A and B Virus Antigens from Respiratory Samples

Reina, Jordi; Padilla, Emma; Alonso, Fermin; Gopegui, Enrique Ruíz de; Munar, María; Mari, Margarita

doi:10.1128/jcm.40.9.3515-3517.2002

Cited by 40 publications

(26 citation statements)

References 20 publications

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“…Half of influenza virus infections can be detected by carrying out DIF on the clinical specimen, while the other half are detected by culture on appropriate cells [109]. Dot blot immunoassays are less sensitive than DIF [109].…”

Section: What Can Antigen Tests Offer In the Diagnosis Of Cap?mentioning

confidence: 99%

Guidelines for the management of adult lower respiratory tract infections

Woodhead¹

2005

European Respiratory Journal

678

555

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Section: What Can Antigen Tests Offer In the Diagnosis Of Cap?mentioning

confidence: 99%

Guidelines for the management of adult lower respiratory tract infections

Woodhead¹

2005

European Respiratory Journal

678

555

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“…In a reference laboratory, there is much less control over sample quality, delays in specimen transport adversely affect both culture and DFA methods (20), and automated techniques are preferred for the handling of large sample numbers. In this setting, real-time RT-PCR has tremendous advantages.…”

Section: Discussionmentioning

confidence: 99%

“…Although rapid shell vial culture is more rapid than standard cell culture, it still requires 2 to 3 days for completion (11,20). Rapid diagnostic methods such as membrane enzyme immunoassay (EIA) and optical immunoassay can provide results in 30 min or less and are easy to perform.…”

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confidence: 99%

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Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus

et al. 2003

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“…A range of different sensitivities for virus isolation have been reported in other studies. Importantly, a small number of studies have described the immediate culture of pediatric patient specimens as having a very high sensitivity for RSV and influenza virus detection when cell culture was performed on site (20,37). In addition to the importance of immediate virus isolation for accurate cell culture results, the methodology is also heterogeneous and the sensitivity for the detection of these viruses differs when different cell culture methods are used (12,27,29,42).…”

Section: Culture Pcrmentioning

confidence: 99%

Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays

et al. 2009

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We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A؉B and Binax NOW RSV assays over two successive respiratory virus seasons. Ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 119 were positive for RSV and 59 were positive for influenza virus. The median age of the patients whose specimens were positive for RSV and influenza virus were 6.3 months and 42.4 years, respectively. The specificity of all of the methods tested was >99%, and the individual sensitivities of NOW RSV, RSV culture, Directigen A؉B, influenza virus culture, and the Proflu-1 PCR for influenza/RSV were 82% (95% confidence interval [CI], 73 to 88), 57% (95% CI, 44 to 69), 59% (95% CI, 44 to 72), 54% (95% CI, 38 to 69), and 98% (95% CI, 93 to 100)/95% (95% CI, 85 to 99), respectively. In a clinical setting where viral isolation is performed to confirm rapid antigen immunoassay results for these common respiratory viruses, one-step real-time reverse transcriptase PCR testing can be a more sensitive and timely confirmatory method.Human respiratory syncytial virus (RSV) and influenza A and B viruses are respiratory pathogens associated with substantial morbidity and mortality annually (43). Virtually all children become infected with RSV within 2 years after birth, and 1% require hospitalization (15). Although the importance of RSV as a cause of pneumonia and brochiolitis in young children is well recognized (21), the most serious morbidity and highest mortality associated with both RSV and influenza virus circulation occurs disproportionately among elderly persons (43). The first-line tests used to detect these virus infections in many hospitals are antigen-based immunoassays. It has been demonstrated that antigen immunoassays have exceedingly poor sensitivity in detecting RSV and influenza virus infections in the elderly, seriously limiting their utility for detecting and confirming institutional or community outbreaks (7,13,38). This study was intended to evaluate the performance of viral isolation in cell culture, one-step real-time multiplex reverse transcription-PCR (RT-PCR), and antigen immunoassays for the detection of influenza virus and RSV in respiratory specimens from adults and children during two respiratory virus seasons. MATERIALS AND METHODS Specimens.Upper respiratory tract specimens were collected from 353 individual symptomatic patients during two successive winter respiratory virus seasons encompassing

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Evaluation of a New Dot Blot Enzyme Immunoassay (Directigen Flu A+B) for Simultaneous and Differential Detection of Influenza A and B Virus Antigens from Respiratory Samples

Cited by 40 publications

References 20 publications

Guidelines for the management of adult lower respiratory tract infections

Guidelines for the management of adult lower respiratory tract infections

Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus

Comparison of Viral Isolation and Multiplex Real-Time Reverse Transcription-PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays

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