Evaluation of a New Commercial Enzyme-Linked Immunosorbent Assay for the                Detection of Porcine Antibodies against <i>Trichinella</i>                Spp.

Frey, Caroline F.; Buholzer, Patrik; Beck, Relja; Tran, Albert; Raeber, Alex J.; Gottstein, Bruno; Schuppers, Manon

doi:10.1177/104063870902100516

Cited by 11 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although ELISA provides a rapid means of detecting specific antibodies, false negatives and/or false positives can be returned, associated with either a delayed onset of the antibody response or the type and quality of the antigen employed. A previous report describes the specificity of the PrioCheck Trichinella Ab ELISA test to be 99.5–99.8% (Frey et al., ). Earlier studies have showed a detection limit of ELISA of 0.01 larvae per gram of tissue (OIE, ), whereas artificial digestion results in a detection limit of approximately 3 larvae per gram of tissue when only 1 g of muscle sample per pig is examined (Forbes and Gajadhar, ).…”

Section: Discussionmentioning

confidence: 99%

A serological Survey ofBrucellaspp.,Salmonellaspp.,Toxoplasma gondiiandTrichinellaspp. in Iberian Fattening Pigs Reared in Free-Range Systems

Hernández¹,

Gómez-Laguna²,

Tarradas

et al. 2013

Transbound Emerg Dis

View full text Add to dashboard Cite

Zoonotic agents such as Brucella spp., Salmonella spp., Toxoplasma gondii and Trichinella spp., all considered high-risk zoonotic pathogens by the European Food Safety Agency (EFSA), may cause no symptoms of infection in free-range pigs yet still have a significant public health impact. A serological survey was therefore performed to determine the history of occurrence of these pathogens in such pigs in southern Spain. A total of 709 serum samples were collected at abattoir from pigs from 79 farms and analysed for specific antibodies against the above pathogens using commercially available ELISA kits. Encysted Trichinella spp. larvae were also sought following the artificial digestion method of diaphragm pillar muscle. The results showed Salmonella spp. to be widely distributed among the sampled herds [73.42%, 95% confidence interval (CI95 ) 65.6-81.78] and Toxoplasma gondii to be present in over half (58.23%, CI95 47.33-69.07). The seroprevalence of Brucella spp. was very low (3.8%, CI95 0.18-7.42), and antibodies against Trichinella spp. were not detected. No encysted Trichinella spp. larvae were microscopically detected.

show abstract

Section: Discussionmentioning

confidence: 99%

A serological Survey ofBrucellaspp.,Salmonellaspp.,Toxoplasma gondiiandTrichinellaspp. in Iberian Fattening Pigs Reared in Free-Range Systems

Hernández¹,

Gómez-Laguna²,

Tarradas

et al. 2013

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…The high sensitivity and specificity were achieved with a lower limit of detection than that of the routine artificial digestion test, suggesting that serological surveillance is a valuable alternative in surveillance for Trichinella spp. in pig production (Frey et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Prevalence of Trichinella spp. antibodies in wild boars (Sus scrofa) and domestic pigs in Korea

et al. 2015

View full text Add to dashboard Cite

ABSTRACT:Trichinellosis is a parasitic zoonosis that is of importance to public health; human trichinellosis usually occurs when improperly cooked pork or wild animal meat is consumed. The purpose of this study was to determine the nationwide seroprevalence of Trichinella infection in wild boar and domestic pig populations in Korea. Using ELISA, we detected no seropositivity among the serum samples of 2350 domestic pigs collected in 2013, indicating that the domestic cycles of Trichinella spp. have disappeared from the domestic pig population in Korea. In contrast, approximately 13% of the 434 wild boars hunted in 2013 were seropositive. Furthermore, the seroprevalence of six of the seven provinces was between 6.7% and 18.3%, indicating that Trichinella infection occurred in the wild boar population throughout the country. The results of this study suggest that Trichinella circulates in the wild boar population and could be transmitted from infected wild boars to other wildlife, domestic pigs, and humans in Korea. Therefore, we recommend continued surveillance of Trichinella infection prevalence in wild animals and an appropriate strategy to prevent human infection in Korea.

show abstract

“…Both surveys used the same commercial ELISA based on E/S antigens as in the present survey but reported higher prevalence rates. In previous studies, this ELISA detected antibodies in samples with larval densities of 0.025 larvae per gram (LPG) [42]. The authors of those validation studies were also able to detect antibodies in pigs infected with different Trichinella species, like T .…”

Section: Discussionmentioning

confidence: 90%

First Report of the Occurrence of Trichinella-Specific Antibodies in Domestic Pigs in Central and Eastern Uganda

et al. 2016

View full text Add to dashboard Cite

Previous research on trichinellosis in Africa focused on isolating Trichinella from wildlife while the role of domestic pigs has remained highly under-researched. Pig keeping in Uganda is historically recent, and evidence on zoonotic pig diseases, including infection with Trichinella species, is scarce. A cross-sectional survey on Trichinella seroprevalence in pigs was conducted in three districts in Central and Eastern Uganda from April 2013 to January 2015. Serum from a random sample of 1125 pigs from 22 villages in Eastern and Central Uganda was examined to detect immunoglobulin G (IgG) against any Trichinella spp. using a commercially available ELISA based on excretory-secretory antigen. ELISA positive samples were confirmed using Western Blot based on somatic antigen of Trichinella spiralis as recommended in previous validation studies. Diaphragm pillar muscle samples (at least 5 g each) of 499 pigs from areas with high ELISA positivity were examined using the artificial digestion method. Overall, 78 of all 1125 animals (6.9%, 95% CI: 5.6–8.6%) tested positive for antibodies against Trichinella spp. in the ELISA at significantly higher levels in Kamuli district compared to Masaka and Mukono districts. Thirty-one percent of the ELISA positive samples were confirmed IgG positive by the Western Blot leading to an overall seroprevalence of 2.1% (95% CI: 1.4–3.2%). The large proportion of ELISA positive samples that could not be confirmed using Western blot may be the result of cross-reactivity with other gastrointestinal helminth infections or unknown host-specific immune response mechanisms in local pig breeds in Uganda. Attempts to isolate muscle larvae for species determination using the artificial digestion method were unsuccessful. Due to the large number of muscle samples examined we are confident that even if pigs are infected, the larval burden in pork is too low to pose a major risk to consumers of developing trichinellosis. This was the first large systematic field investigation of Trichinella infection in domestic pigs in Uganda and its results imply that further studies are needed to identify the Trichinella species involved, and to identify potential sources of infection for humans.

show abstract

Evaluation of a New Commercial Enzyme-Linked Immunosorbent Assay for the Detection of Porcine Antibodies against Trichinella Spp.

Cited by 11 publications

References 16 publications

A serological Survey ofBrucellaspp.,Salmonellaspp.,Toxoplasma gondiiandTrichinellaspp. in Iberian Fattening Pigs Reared in Free-Range Systems

A serological Survey ofBrucellaspp.,Salmonellaspp.,Toxoplasma gondiiandTrichinellaspp. in Iberian Fattening Pigs Reared in Free-Range Systems

Prevalence of Trichinella spp. antibodies in wild boars (Sus scrofa) and domestic pigs in Korea

First Report of the Occurrence of Trichinella-Specific Antibodies in Domestic Pigs in Central and Eastern Uganda

Contact Info

Product

Resources

About