Evaluation of a new chromogenic medium, Uriselect 4, for the isolation and identification of urinary tract pathogens

Perry, John D.; Butterworth, Lynne A.; Nicholson, A.; Appleby, Martin; Orr, K.E.

doi:10.1136/jcp.56.7.528

Cited by 27 publications

(15 citation statements)

References 10 publications

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“…This finding is in keeping with previous studies demonstrating the increased ability of chromogenic media to isolate mixed growth of uropathogens from urine specimens (23,25). This is further supported in our study by the isolation of P. mirabilis from one urine specimen in significant amounts with E. coli from chromogenic agar, while E. coli alone was isolated from conventional media.…”

Section: Discussionsupporting

confidence: 81%

Culture of Urine Specimens by Use of chromID CPS Elite Medium Can Expedite Escherichia coli Identification and Reduce Hands-On Time in the Clinical Laboratory

et al. 2016

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Urine is one of the most common specimen types submitted to the clinical microbiology laboratory; the use of chromogenic agar is one method by which the laboratory might expedite culture results and reduce hands-on time and materials required for urine culture analysis. The objective of our study was to compare chromID CPS Elite (bioMérieux), a chromogenic medium, to conventional primary culture medium for evaluation of urine specimens. Remnant urine specimens (n ‫؍‬ 200) were inoculated into conventional media and into chromID CPS Elite agar (chromID). The time to identification and consumables used were documented for both methods. Clinically significant pathogen(s) were recovered from 51 cultures using conventional media, with Escherichia coli being the most frequently recovered organism (n ‫؍‬ 22). The rate of exact uropathogen agreement between conventional and chromogenic media was 82%, while overall categorical agreement was 83.5% The time interval between plating and final organism identification was decreased with chromID agar versus conventional media for E. coli (mean of 24.4 h versus 27.1 h, P < 0.001). Using chromID, clinically significant cultures required less hands-on time per culture (mean of 1 min and 2 s [1:02 min]) compared to conventional media (mean of 1:31 min). In addition, fewer consumables (2.4 versus 3.3 sticks and swabs) and rapid biochemical tests (1.0 versus 1.9) were necessary using chromID versus conventional media. Notably, antimicrobial susceptibility testing demonstrated good overall agreement (97.4%) between the chromID and conventional media for all antibiotics tested. chromID CPS Elite is accurate for uropathogen identification, reduces consumable usage, and may expedite the identification of E. coli in clinical specimens. U rinary tract infections (UTIs) are among the most common bacterial infections worldwide (1). Screening urine cultures to identify significant uropathogens can require considerable hands-on time, consumable usage, and biochemical testing. As a result, urine cultures to support a diagnosis of UTI account for a major share of the workload in microbiology laboratories (2), which are facing a shortage of skilled laboratory personnel (3). Thus, there is a need for accurate methods that save time and reduce workload. In addition, there is growing emphasis on expediting culture results from patients with infection; minimizing the window of diagnostic uncertainty is essential to optimize antimicrobial therapy and improve patient outcomes (4).One approach to streamline urine culture is the rapid identification of organisms from growth on solid agar using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). This method has been shown to be accurate and cost-effective for identification of bacterial and fungal organisms (5-15). However, considerable hands-on time may be necessary upstream of MALDI-TOF MS to isolate significant uropathogens from standard media, particularly for patients with mixed infections.The majority of com...

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Section: Discussionsupporting

confidence: 81%

Culture of Urine Specimens by Use of chromID CPS Elite Medium Can Expedite Escherichia coli Identification and Reduce Hands-On Time in the Clinical Laboratory

et al. 2016

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“…In addition, in most retrospective studies standard urine cultures were screened for candiduria, which means that urine was cultured on MacConkey and blood agar only. Some laboratories culture urines on Uriselect agar, which is a chromogenic agar that allows the preliminary identification of predominantly bacterial uropathogens (195). Although these culture methods are certainly sufficient to identify bacteria, they may be significantly less sensitive to recover C. albicans and non-C. albicans species.…”

Section: Definition and Diagnosis Of Candiduriamentioning

confidence: 99%

Candida Infections of the Genitourinary Tract

2010

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SUMMARYAll humans are colonized withCandidaspecies, mostlyCandida albicans, yet some develop diseases due toCandida, among which genitourinary manifestations are extremely common. The forms of genitourinary candidiasis are distinct from each other and affect different populations. While vulvovaginal candidiasis affects mostly healthy women, candiduria occurs typically in elderly, hospitalized, or immunocompromised patients and in neonates. Despite its high incidence and clinical relevance, genitourinary candidiasis is understudied, and therefore, important questions about pathogenesis and treatment guidelines remain to be resolved. In this review, we summarize the current knowledge about genitourinary candidiasis.

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“…(2003) compared three chromogenic agars with traditional CLED agar and found that all three detected significantly more mixed growths than CLED agar ( P < 0·01). Similary, Perry et al. (2003a) showed that, of 168 confirmed mixed cultures, only 78% were recognized as polymicrobial on CLED medium compared with 99% on Uriselect 4 and 97% on CPS ID2.…”

Section: Detection Of Urinary Tract Pathogensmentioning

confidence: 86%

The application of chromogenic media in clinical microbiology

Perry

Freydière

2007

Journal of Applied Microbiology

105

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Summary Since 1990, a wide range of chromogenic culture media has been made commercially available providing useful tools for diagnostic clinical microbiology. By the inclusion of chromogenic enzyme substrates targeting microbial enzymes, such media are able to target pathogens with high specificity. Examples of target pathogens include Staphylococcus aureus, Streptococcus agalactiae, Salmonella spp. and Candida spp. The inclusion of multiple chromogenic substrates into culture media facilitates the differentiation of polymicrobial cultures, thus allowing for the development of improved media for diagnosis of urinary tract infections and media for the enhanced discrimination of yeasts. The purpose of this review is to provide some insight into how such media work and appraise their utility in routine clinical diagnostics, in comparison with conventional media.

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Evaluation of a new chromogenic medium, Uriselect 4, for the isolation and identification of urinary tract pathogens

Cited by 27 publications

References 10 publications

Culture of Urine Specimens by Use of chromID CPS Elite Medium Can Expedite Escherichia coli Identification and Reduce Hands-On Time in the Clinical Laboratory

Culture of Urine Specimens by Use of chromID CPS Elite Medium Can Expedite Escherichia coli Identification and Reduce Hands-On Time in the Clinical Laboratory

Candida Infections of the Genitourinary Tract

The application of chromogenic media in clinical microbiology

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