Evaluation of a New Antigen Capture ELISA for Detection and Characterization of Platelet Alloantibodies

Boehlen, Françoise; Bulla, Oana; Moerloose, Philippe de

doi:10.1016/s0049-3848(98)00107-8

Cited by 6 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our ACE and MAIPA assay data presented here suggest that the conformational changes induced in platelet membrane-bound aIIbb3 following exposure to EDTA do not occur with immobilized integrin (as used in ACE assays). However, whilst the data from the one example of anti-HPA-1a used in this workshop suggest that immobilized aIIbb3 is perhaps a more suitable target for detection of anti-HPA-1a, these results should be interpreted with caution since other studies have shown that some MAIPAreactive examples of anti-HPA-1a are undetectable in ACE assays [12,21,22]. Regardless, these data clearly show that integrin conformation plays a key role in the sensitive detection of HPA-1a antibodies.…”

Section: Discussionmentioning

confidence: 77%

Sensitivity of assays for the detection of HPA‐1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays

Allen

Metcalfe

Kaplan³

et al. 2013

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 77%

Sensitivity of assays for the detection of HPA‐1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays

Allen

Metcalfe

Kaplan³

et al. 2013

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

“…The superior performance of anti‐IgG over anti‐IgG/A/M conjugate for HPA Ab detection in commercial ACEs was originally reported by Boehlen et al . (1998 ) (and recently confirmed by Lucas & Rogers, 1999), but the reasons for the difference in conjugate performance have not previously been fully analysed.…”

Section: Conclusion and Recommendationsmentioning

confidence: 67%

“…GTI Pak‐Plus, Pak‐One and Pak‐12) arises because their use: (i) avoids the problem of extensive validation inherent in the production of in‐house versions of such kits; (ii) offers a less complex viable alternative to the MAIPA for detecting platelet‐glycoprotein specific antibodies; (iii) generates results in a fraction of the time needed to complete the MAIPA. However, few published studies comparing the sensitivity of ACE vs. MAIPA exist ( Boehlen et al ., 1998 ; Lucas & Rogers, 1999).…”

mentioning

confidence: 99%

“…One problem in the use of commercial ACE kits (e.g. GTI QuickScreen), either for HLA or HPA Ab detection, has been the failure of anti‐IgG/A/M conjugates to detect all HLA class I Ab defined using cytotoxicity tests ( Lucas et al ., 1997 ) or all HPA Ab detectable using the MAIPA ( Boehlen et al ., 1998 ; Lucas & Rogers, 1999). We were concerned that the anti‐IgG/A/M conjugate routinely supplied with ACE kits could fail to detect all platelet‐reactive (i.e.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Antigen capture ELISA for platelet antibody detection: choice of conjugate influences assay result

Lubenko¹,

Savage²

2000

Transfusion Medicine

View full text Add to dashboard Cite

The performance of an anti-IgG/A/M and two anti-IgG alkaline phosphatase (AP) conjugates in a commercial antigen capture ELISA (ACE) were compared for their ability to detect antibodies to human platelet antigens (HPAs) contained in 11 sera. Three anti-HPA-1a and one anti-HPA-3a were not detected by the anti-IgG/A/M conjugate, but both anti-IgG conjugates detected all HPA antibodies as a consequence of increased sensitivity in detecting specific antibody binding. This increase varied from 10% to 500%, depending on the serum being tested. The increased sensitivity in some instances was also accompanied by an apparent increase in nonspecific binding, as measured by activity against irrelevant glycoproteins that should not have been recognized by the relevant HPA antibodies in the sera in question. Hence, anti-IgG conjugates would appear to be preferable for detecting platelet-reactive antibodies in many clinical situations; however, the choice of anti-IgG conjugate should be made judiciously (and appropriately validated), in order to avoid false positive results arising from increased nonspecific binding, which may otherwise be erroneously attributed to the presence of auto-antibodies in the serum under investigation.

show abstract

“…Detection of anti‐HPA is required for the diagnosis. Conventional immunoenzymatic assays currently used such as the monoclonal antibody‐specific immobilization of PLT antigens or modified antigen capture enzyme‐linked immunosorbent assay assays may fail to detect the alloantibody in case of low‐affinity or ‐avidity antibodies or unstable antigenic presentation on the panel PLTs . Alternative methods have been proposed, among them surface plasmon resonance .…”

Section: How May I Confirm the Diagnosis?mentioning

confidence: 99%

How do we treat fetal and neonatal alloimmune thrombocytopenia?

Bertrand

Kaplan

2014

Transfusion

View full text Add to dashboard Cite

Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a potentially devastating disease, seen in one in 800 to 1000 neonates. FNAIT is the most common cause of early-onset isolated severe neonatal thrombocytopenia in maternity wards. The complication of this disorder most to be feared is intracranial hemorrhage, leading to death or to neurologic sequels. As there is no systematic screening of at-risk pregnancies, FNAIT is often discovered when signs of bleeding are observed during pregnancy or at delivery. Platelet transfusion is required in case of bleeding or severe thrombocytopenia (<30 × 10(9) /L) during the 48-hour-postdelivery period. Diagnosis of alloimmunization is important for management of the index case and for subsequent pregnancies, due to the increasing severity of this syndrome as it recurs. Noninvasive antenatal therapy is based on maternal perfusion of intravenous immunoglobulins and risk stratification. In our experience, the addition of corticoids during the last trimester significantly improves the efficiency of treatment. Follow-up of antibody concentration during pregnancy may constitute a useful variable for therapy effectiveness.

show abstract

Evaluation of a New Antigen Capture ELISA for Detection and Characterization of Platelet Alloantibodies

Cited by 6 publications

References 25 publications

Sensitivity of assays for the detection of HPA‐1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays

Sensitivity of assays for the detection of HPA‐1a antibodies: results of an international workshop demonstrating the impact of cation chelation from integrin αIIbβ3 on three widely used assays

Antigen capture ELISA for platelet antibody detection: choice of conjugate influences assay result

How do we treat fetal and neonatal alloimmune thrombocytopenia?

Contact Info

Product

Resources

About