Evaluation of a natural compound extracted from Dolichandrone atrovirens as a novel antioxidant agent using Caenorhabditis elegans

Abstract

The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p<0.0001 (treatment v/s control group). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide–Formazan (MTT) assay results showed that treatment had better survival rates than the control group at different time intervals i.e. 48 h, and 72 h with p<0.01. Mechanosensation (behavioral study) as well as in vivo study results showed that at 0 h, 10 μg/ml of DAM-1 treatment showed a better anti-oxidative activity than the control group, 25 and 50 μg/ml of DAM-1 treated groups with p<0.001 but at 2.5 h incubation with 10, 25, 50 μg/ml of DAM-1 showed an increased anti-oxidative activity than the control group with p<0.001. Thermoresistance assay confirmed that the treatment group had more survival than control group with p<0.001. Absorption study of DAM-1 in C. elegans has shown that the absorption of the drug increases up to 180 mins with a slight decrease after 360 mins and then constant absorption up to 1440 mins. This study paves the way towards the initiative to explore the pharmacological role of DAM-1 in various oxidative stress mediated diseases at molecular levels and the absorption study points out its potential role which could be utilized in the metabolomics and proteomics analysis of this compound in other studies.

Icariin Improves Stress Resistance and Extends Lifespan in Caenorhabditis elegans through hsf-1 and daf-2-Driven Hormesis

“…To assess the safety of icariin on nematodes, a viability assay was conducted [ 22 ]. During the larval stages (L1-L4), nematodes were subjected to a 48 h incubation period with icariin at concentrations ranging from 10 to 200 μM ( Figure 1 a).…”

“…The experimental treatment was added to heat-inactivated and concentrated 10-fold E. coli OP50 at final concentrations of 10, 50, and 100 μM. The experimental concentrations were selected following evaluation of the viability of the N2 and daf-2 nematodes for 48 h by MMT-based assay [ 22 ].…”

Xanthine oxidase inhibition study of isolated secondary metabolites from Dolichandrone spathacea (Bignoniaceae): In vitro and in silico approach

“…Various species within the Dolichandrone genus have been studied for their medicinal properties, encompassing many benefits. These properties include anticancer, anti-inflammatory, hepatoprotective, neuroprotective, hypolipidemic, anti-fever, hypoglycemic, antimicrobial, anxiolytic, anti-mutagenic, and antioxidant activities ( Badgujar and Surana, 2010 , Nguyen et al, 2018 , Chaimontri et al, 2021 , Yellurkar et al, 2021 ). The present study is the first to identify and characterize the phytochemical constituents with potential XO inhibition of D. spathacea .…”

Betulinic acid counteracts the lipid accumulation in Caenorhabditis elegans by modulation of nhr-49 expression

“…In order to assess the viability of the nematodes upon treatment for 48 h, we applied the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyl tetrazolium bromide (MTT) assay [16].…”