Evaluation of a nanophosphor lateral-flow assay for self-testing for herpes simplex virus type 2 seropositivity

Goux, Heather J.; Raja, Balakrishnan; Kourentzi, Katerina; Trabuco, João R. C.; Vu, Binh; Paterson, Andrew S.; Kirkpatrick, A. F.; Townsend, Blane; Lee, Miles; Truong, Van Thi Thanh; Pedroza, Claudia; Willson, Richard C.

doi:10.1371/journal.pone.0225365

Cited by 18 publications

(18 citation statements)

References 29 publications

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“…Another lateral flow assay test was developed by Goux et al to detect HSV-2 infections, with enhanced sensitivity using luminescent nanoparticles (nanophosphor) tagged with goat anti-human antibodies, as shown in Figure 5 [ 167 ]. The assay strip consists of a test line of recombinant glycoprotein G from HSV-2, a control line of goat anti-human antibodies.…”

Section: Detection Of Viral Antibodies In Blood (Serological Assays)mentioning

confidence: 99%

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Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

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Section: Detection Of Viral Antibodies In Blood (Serological Assays)mentioning

confidence: 99%

“…( B ) Images of the serum/plasma panel tested with the HSV-2 PLNP LFA strips, captured using an iPhone 7 smartphone with a preinstalled LFA iPhone app showing phosphor emission. (Panel no 16* was re-tested at a higher dilution due to weaker control line) [ 167 ].…”

Section: Figurementioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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“…The down-conversion carboxyl-capped YVO 4 :Eu,Bi has been used as a label for the quantitative analysis of human IgG [ 2 ]. Persistent luminescence nanophosphors, such as strontium aluminate [ 42 , 43 ] SrAl 2 O 4 :Eu 2+ ,Dy 3+ , and strontium silicide [ 44 ] (Sr 0.625 Ba 0.375 ) 2 MgSi 2 O 7 :Eu 2+ ,Dy 3+ , are also suitable labels for LFIA. LFIA labeled SrAl 2 O 4 :Eu 2+ ,Dy 3+ showed a high sensitivity of 96.7% and a specificity of 100% for the detection of herpes simplex virus type 2 (HSV-2) infection [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

“…Persistent luminescence nanophosphors, such as strontium aluminate [ 42 , 43 ] SrAl 2 O 4 :Eu 2+ ,Dy 3+ , and strontium silicide [ 44 ] (Sr 0.625 Ba 0.375 ) 2 MgSi 2 O 7 :Eu 2+ ,Dy 3+ , are also suitable labels for LFIA. LFIA labeled SrAl 2 O 4 :Eu 2+ ,Dy 3+ showed a high sensitivity of 96.7% and a specificity of 100% for the detection of herpes simplex virus type 2 (HSV-2) infection [ 42 ]. LFIA labeled with up-conversion NaYF 4 :Yb 3+ ,Er 3+ also showed a high sensitivity of 99.19% and a specificity of 95.08% for detecting the hepatitis B surface antibody (HBsAb), as reported by Li et al[ 45 ].…”

Section: Introductionmentioning

confidence: 99%

“…The down-conversion carboxylcapped YVO 4 :Eu,Bi has been used as a label for the quantitative analysis of human IgG [2]. Persistent luminescence nanophosphors, such as strontium aluminate [42,43] 3+ showed a high sensitivity of 96.7% and a specificity of 100% for the detection of herpes simplex virus type 2 (HSV-2) infection [42]. LFIA labeled with up-conversion NaYF 4 :Yb 3+ ,Er 3+ also showed a high sensitivity of 99.19% and a specificity of 95.08% for detecting the hepatitis B surface antibody (HBsAb), as reported by Li et al [45].…”

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confidence: 99%

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Red Zn2SiO4:Eu3+ and Mg2TiO4:Mn4+ nanophosphors for on-site rapid optical detections: Synthesis and characterization

Yang¹,

Zhang²,

Huang

et al. 2021

Appl. Phys. A

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This study reports the synthesis and characterization of the red nanophosphors Zn 2 SiO 4 :Eu 3+ (ZSO:Eu 3+ ) and Mg 2 TiO 4 :Mn 4+ (MTO:Mn 4+ ). The use of phosphors as a fluorescence label for lateral flow immunochromatographic assay (LFIA) has also been described. The optimal photoluminescence (PL) for ZSO:Eu 3+ was obtained when it was synthesized with 7 mol% of Eu 3+ and annealed at 1100 °C for 1 h. Long fluorescence lifetime (1.01 ms), high activation energy E a (0.28 eV), and low PL degeneration (10% at 110 °C) are the characteristics of ZSO:Eu 3+ . MTO:Mn 4+ also exhibited high PL intensity along with a high E a of 0.32 eV. The emission wavelengths of phosphors are biocompatible with the optical bio-window of tissues. When human immunoglobulin G (human IgG) at a constant concentration of 100 μg/mL was used for detection, the PL ratios of the test line to the control line were 2.15 and 2.28 for the ZSO:Eu 3+ -and MTO:Mn 4+ -labeled LFIA, respectively. Thus, the ZSO:Eu 3+ and MTO:Mn 4+ nanophosphors are capable of human IgG recognition and are the promising candidates as fluorescent labels for on-site rapid optical biodetection.

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