Evaluation of a Multiplexed Bead Assay for Assessment of Epstein-Barr Virus Immunologic Status

Klutts, J. Stacey; Liao, Robert S.; Dunne, W. Michael; Gronowski, Ann M.

doi:10.1128/jcm.42.11.4996-5000.2004

Cited by 46 publications

(35 citation statements)

References 13 publications

(20 reference statements)

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“…This suggests that the interpretive patterns shown here are specific to use with BioPlex results. However, in this study and two previous studies (3,7), there was very strong (92 to 97%) concordance between BioPlex 2200 and predicate assay results. Therefore, Tables 4 and 6 may also be useful for interpretation of EBV serologic results generated with other assays, but further studies obviously are needed to support that hypothesis.…”

Section: Discussionsupporting

confidence: 80%

“…However, the association between a particular EBV diagnosis and serological patterns is based largely on assumptions. While utilization of multiplex technology for the detection of EBV antibodies has been evaluated recently (1,3,7,9,12), this is the first evidence-based study of its kind aiming to match a large number of clinical diagnoses with EBV serological patterns. Here, we analyzed almost 1,850 specimens, generating Ͼ10,000 individual serological test results, and reviewed the medical records of more than 800 patients.…”

Section: Discussionmentioning

confidence: 99%

“…Bio-Rad Laboratories (Hercules, CA) recently introduced a multiplex platform (BioPlex 2200) that can measure all five of these antibodies from one sample in two reaction vessels. We and others recently evaluated this platform with regard to its performance against various predicate assays and showed that it performed quite well (3,7).…”

mentioning

confidence: 99%

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Evidence-Based Approach for Interpretation of Epstein-Barr Virus Serological Patterns

et al. 2009

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Diagnosis of Epstein-Barr virus (EBV) infection is based on clinical symptoms and serological markers, including the following: immunoglobulin G (IgG)and IgM antibodies to the viral capsid antigen (VCA), heterophile antibodies, and IgG antibodies to the EBV early antigen-diffuse (EA-D) and nuclear antigen (EBNA-1). The use of all five markers results in 32 possible serological patterns. As a result, interpretation of EBV serologies remains a challenge. The purpose of this study was to use a large population of patients to develop evidence-based tools for interpreting EBV results. This study utilized 1,846 serum specimens sent to the laboratory for physician-ordered EBV testing. Chart review was performed for more than 800 patients, and diagnoses were assigned based on physician-ordered testing, clinical presentation, and patient history. Testing for all five EBV antibodies was performed separately on all serum samples using the Bio-Rad BioPlex 2200 system. Presumed EBV diagnosis (based on previous publications) was compared to EBV diagnosis based on a medical record review for each serological pattern. Interestingly, of the 32 possible serological patterns, only 12 occurred in >10 patients. The remaining 20 patterns were uninterpretable because they occurred with such infrequency. Two easy-to-use tables were created to interpret EBV serological patterns based on whether three (EBV VCA IgG, IgM, and heterophile) or five markers are utilized. The use of these two tables allows for interpretation of >95% of BioPlex serological results. This is the first evidence-based study of its kind for EBV serology.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Evidence-Based Approach for Interpretation of Epstein-Barr Virus Serological Patterns

et al. 2009

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“…In some cases exanthematous reactions triggered by EBV can be mistaken with an eruption of rubella (2). Moreover, EBV infection is shown to be associated with lymphoproliferative disorders in patients who are suffering from immunodefi ciency, Burkitt lymphoma, and nasopharyngeal carcinoma (3,4). Infectious mononucleosis is also caused by infection of B cells by EBV and is generally diagnosed by serological methods.…”

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confidence: 99%

Determination of serological profiles and avidity of specific antibodies in the sera of patients with potential Epstein-Barr virus (EBV) infection

Akpolat¹,

Gedik²,

Nergiz³

et al. 2013

BLL

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Abstract:Background: Epstein-Barr Virus, frequently referred to as EBV, is a member of the herpesvirus family and one of the most common human viruses. The virus occurs worldwide, and most people become infected with EBV sometime during their lives. Objectives: The purpose of this study was to investigate the serological profi les of specifi c antibodies among the sera of suspected EBV infection patients along with VCA-IgG avidity. Methods: A total of 522 patient's sera were sent to The Clinical Microbiology Laboratory for EBV specifi c antibody detection and were studied by IFA method during a two year period. The serum samples were tested for EBV specifi c VCA IgG, VCA IgM, EA, EBNA antibodies and VCA IgG aviditity. Results: Among 33 patients those who had low avidity for VCA IgG, 27 (81.8 %) of them had a serologic profi le as follows; positive VCA IgG, negative VCA IgM, negative EA and negative EBNA. Conclusion: While this profi le is considered as a primary infection, the frequency of the coexistence of VCA IgG low avidity with this profi le is interepreted that avidity may lead to detect primary infection (Tab. 2, Ref. 25 Abbreviations: IFA -indirect fl uorescent antibody, EBV -Epstein-Barr virus, EA -early antigen,VCA -viral capsid antigen, EBNA -epstein-barr nuclear antigen.Epstein-Barr virus, commonly referred to as EBV, is a human specifi c member of the herpesvirus family and is a B-cell lymphotropic virus. An EBV infection occurs by close contact with EBV presented in oral secretions. The virus initially wells in the oropharyngeal epithelial cells and causes persistent infections. Seroepidemiologic studies indicate that EBV infections are common in all communities and occur worldwide, and most people become infected with EBV sometime during their lives. In Europe including Turkey, as many as 70-80 % of adolescents and 80-90 % of adults are reported to be seropositive for EBV (1).The course of EBV infection can vary with regard to the age and health status of the person. In young children host-virus infection can take place with no apparent symptoms. By contrast, in 30-50 % of the adult patients EBV infection can cause diffuse lymphadenopathy, hepatomegaly, splenomegaly, and tonsillitis. In some cases exanthematous reactions triggered by EBV can be mistaken with an eruption of rubella (2). Moreover, EBV infection is shown to be associated with lymphoproliferative disorders in patients who are suffering from immunodefi ciency, Burkitt lymphoma, and nasopharyngeal carcinoma (3,4). Infectious mononucleosis is also caused by infection of B cells by EBV and is generally diagnosed by serological methods. EBV serology is utilized to help distinguish EBV reactivation infections from primary EBV infections and to demonstrate acute EBV infections. During this process, it is critically important to perform the tests at the same time for the presence of EBV-VCA IgM, EBV-VCAIgG, anti-EBV-EA, anti EBV-EBNA and VCA IgG avidity (2).The spectrum of antibody assays comprises unspecifi c tests, such as the long-known te...

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“…This report describes the development of a new toxoplasma IgM immunoassay that utilizes the Bio-Rad BioPlex 2200 immunoassay analyzer, a fully automated platform with multiplex capabilities (10). The data demonstrate that the conditions employed for the immunoassay yielded a differential response to T. gondii-specific IgM antibodies, with reduced sensitivity to IgM from samples with high-avidity IgG antibodies.…”

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confidence: 99%

Detection of Immunoglobulin M Antibodies Specific for Toxoplasma gondii with Increased Selectivity for Recently Acquired Infections

2004

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Toxoplasma gondii infections can cause serious complications in pregnant women, leading to miscarriage, stillbirth, and birth defects. Definitive diagnosis of T. gondii acute infection is therefore critical for the clinical management of a mother and her fetus. Positive immunoglobulin M (IgM) results are not sufficient as evidence of recent infection, as these antibodies are often present for many months. Further, IgG avidity and differential agglutination tests, two tests used by reference laboratories to distinguish between recent and past infections, are not always in agreement, and both methods yield a significant number of indeterminate results. We report the development of a new toxoplasma IgM immunoassay that is performed by using a bead-based immunoassay on an automated analyzer (BioPlex 2200). Initial validation included 204 samples from pregnant women and 198 samples from asymptomatic healthy adults. An overall specificity of 99% was observed. Further, 100% sensitivity for acute infections was observed for 10 well-characterized seroconversion panels. We then examined 50 samples from pregnant women, all of which were IgM positive by ELISA, which had been fully evaluated in a reference laboratory. Of the 50 samples, 34 (68%) tested positive in the BioPlex 2200 toxoplasma IgM assay, of which 32 of 34 (94%) exhibited an acute or equivocal pattern by differential agglutination. Of the 16 negative samples, 15 (94%) showed high-IgG-avidity antibodies. Collectively, these results suggest that this new toxoplasma assay shows a preferential response to IgM antibodies produced by recent infections, reducing the number of positive results for pregnant women that will require extensive additional clinical evaluation.

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Evaluation of a Multiplexed Bead Assay for Assessment of Epstein-Barr Virus Immunologic Status

Cited by 46 publications

References 13 publications

Evidence-Based Approach for Interpretation of Epstein-Barr Virus Serological Patterns

Evidence-Based Approach for Interpretation of Epstein-Barr Virus Serological Patterns

Determination of serological profiles and avidity of specific antibodies in the sera of patients with potential Epstein-Barr virus (EBV) infection

Detection of Immunoglobulin M Antibodies Specific for Toxoplasma gondii with Increased Selectivity for Recently Acquired Infections

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