Evaluation of a monoclonal antibody for detection ofHelicobacter pylori in a direct immunofluorescence test

Rodewig, U.; Bemb, W.; Bitter‐Suermann, D.; Elsheikh, Mamoun; Fritsch, Sabine; Glenn-Calvo, Eduardo; Soudah, B.; Varrentrapp, M; Wagner, Siegfried; Bär, Werner

doi:10.1007/bf01989981

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Both polymerase chain reaction and fluorescent antibody staining have been used successfully for the detection of H. pylori in clinical or environmental samples (Rivera et al . 1991; Rodewig et al . 1992; Husson et al .…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Helicobacter pylori in surface water in the United States

1999

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The primary mode of transmission of the human pathogen Helicobacter pylori is unresolved. This study examined the possibility that H. pylori is water‐borne. Because methods for the direct culture of H. pylori from water samples remain elusive, a microscopic technique was used for detection of this organism. Actively respiring micro‐organisms binding monoclonal anti‐H. pylori antibody were found in the majority of surface and shallow groundwater samples tested (n = 62), indicating that H. pylori may be present in aquatic environments in the US and supporting a water‐borne route of transmission for this organism. There was no significant correlation between the occurrence of either total coliforms or Escherichia coli in the water and the presence of H. pylori. Our results indicate that routine screening of water supplies for the presence of traditional indicator organisms may fail to protect the consumer from exposure to H. pylori.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Helicobacter pylori in surface water in the United States

1999

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“…Specific monoclonal antibodies have been described for identifying Lawsonia intracellularis (101), H. pylori (9,43,81,143), and H. pylori and H. mustelae collectively (81). Antibodies with fluorescent labels have been used to demonstrate L. intracellularis and H. pylori directly in infected tissue (43,81,101,143), but fluorimetric devices are required for this purpose and also for identifying the bacteria in culture. In contrast, the chromogenic immunoblot assay (9) yields unequivocal results and does not require special optical equipment for use.…”

Section: Serological Testsmentioning

confidence: 99%

Identification methods for campylobacters, helicobacters, and related organisms

1996

Clin Microbiol Rev

265

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The organisms which are referred to as campylobacteria are associated with a diverse range of diseases and habitats and are important from both clinical and economic perspectives. Accurate identification of these organisms is desirable for deciding upon appropriate therapeutic measures, and also for furthering our understanding of their pathology and epidemiology. However, the identification process is made difficult because of the complex and rapidly evolving taxonomy, fastidious nature, and biochemical inertness of these bacteria. These problems have resulted in a proliferation of phenotypic and genotypic methods for identifying members of this group. The purpose of this review is to summarize the problems associated with identifying campylobacteria, critically appraise the methods that have been used for this purpose, and discuss prospects for improvements in this field.

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“…Several other MAbs which react with H. pylori-specific antigens have been described (3,(5)(6)(7)(8)17). Some of these MAbs have been used for diagnostic purposes and have been employed in immunofluorescence tests (7,8,17). Protein antigens with molecular weights of 19,000 and 25,000 recognized by two of these MAbs have been identified (6,7).…”

Section: Vol 33 1995mentioning

confidence: 99%

Identification of Helicobacter pylori by immunological dot blot method based on reaction of a species-specific monoclonal antibody with a surface-exposed protein

1995

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Monoclonal antibodies (MAbs) against membrane preparations of Helicobacter pylori were produced. One MAb was found to be specific for H. pylori, because it did not react with a number of other bacterial species, including Helicobacter felis and Campylobacter jejuni. This MAb reacted with a 30-kDa protein found in outer membrane preparations of H. pylori. The protein was also detected on the cell surface on intact bacteria when analyzed by immunoelectron microscopy. To facilitate the identification of H. pylori isolates after culturing of biopsies, an immunodot blot assay based on the reaction of this MAb was developed. This assay was found to be highly specific for H. pylori. Sixty-six clinical isolates typed as H. pylori by conventional biochemical tests were found to be positive, whereas no other bacterial species tested gave a positive result. By this method, reliable and rapid identification of H. pylori could be accomplished.

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Evaluation of a monoclonal antibody for detection ofHelicobacter pylori in a direct immunofluorescence test

Cited by 4 publications

References 13 publications

Occurrence of Helicobacter pylori in surface water in the United States

Occurrence of Helicobacter pylori in surface water in the United States

Identification methods for campylobacters, helicobacters, and related organisms

Identification of Helicobacter pylori by immunological dot blot method based on reaction of a species-specific monoclonal antibody with a surface-exposed protein

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