Evaluation of a Monoclonal Antibody, BC‐1, Which Identifies an Antigen Expressed on the Surface Membrane of Human Extravillous Trophoblast

Loke, Y.W.; Hsi, Bae‐Li; Bulmer, Judith N.; Grivaux, Catherine; Hawley, Sasha; Gardner, Lucy; King, Ashley; Carter, N. P.

doi:10.1111/j.1600-0897.1992.tb00727.x

Cited by 23 publications

(8 citation statements)

References 6 publications

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“…2A) and concurrent changes of intermediate filament composition and morphological structures are informative regarding cellular migration direction in general, we hypothesise that, in human placenta, intra-arterial trophoblast cells reach their locus in the junctional zone by migration through decidual tissue and by consecutive intravasation. This supports the opinion of many authors (Kurman et al 1984;Damsky et al 1992;Loke et al 1992;Fisher and Damsky 1993) that, in human, the intra-arterial trophoblast population is cell-column-derived and invades by migration through tissue and not along the inner surface of the uteroplacental artery.…”

Section: Cytokeratin 17 Profile Of Junctional Zone Trophoblastsupporting

confidence: 87%

“…Intramural and intra-arterial trophoblast cells reach their final location by migration either through the maternal decidual stroma (interstitial trophoblasts) or along the inner surface of uteroplacental arteries (intraarterial or endovascular trophoblasts; for a review, see Benirschke and Kaufmann 1995). Most authors suggest that trophoblastic invasion of the uteroplacental arteries in humans takes place as the final step of trophoblastic invasion of the basal plate, starting at the cell columns (Kurman et al 1984;Damsky et al 1992;Loke et al 1992;Fisher and Damsky 1993). We have studied trophoblast subpopulations with respect to their morphology and cytokeratin expression and have examined their specific migration pathways in junctional zone tissue and in uteroplacental arteries.…”

Section: Introductionmentioning

confidence: 98%

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Cytokeratin 17 as an immunohistochemical marker for intramural cytotrophoblast in human first trimester uteroplacental arteries

Pröll

Blaschitz

Hartmann

et al. 1997

Cell and Tissue Research

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Trophoblast cells, as blastocyst-wall derivatives, are of epithelial origin and differentiate initially into syncytiotrophoblast and cytotrophoblast subpopulations. Cyto- and syncytiotrophoblasts are the two cell types present in the surface cell layers of placental villi. Cytotrophoblastic cells lie in contact with the basal lamina and are the proliferating stem cells that guarantee cytotrophoblast and syncytiotrophoblast persistence. Implantation and placenta formation are mainly based on these two cell types. Villous cytotrophoblasts are the stem cells for all extravillous trophoblast subpopulations, which exhibit strictly regulated invasiveness. One aspect of extravillous trophoblasts is that they invade maternal endometrial spiral arteries and dilate them in order to achieve sufficient fetal blood supply. During this process, trophoblast cells, which are located in the remodelled uteroplacental artery walls, are thus defined as intramural cytotrophoblasts. Trophoblast differentiation is accompanied and defined by alterations in, for example, the translation pattern for cytokeratin genes. In an immunohistochemical study, we have demonstrated that only intramural cytotrophoblasts, from all the trophoblast populations of the junctional zone, express cytokeratin 17. Furthermore, cell shape and vascular architecture indicate that, in human placenta, intra-arterial trophoblast cells reach their destination by migration through the endometrial interstitium with consecutive intravasation. Cytokeratin 17, in particular, can therefore be used as a specific immunohistochemical marker for the intramural trophoblast subpopulation.

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Section: Cytokeratin 17 Profile Of Junctional Zone Trophoblastsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 98%

Cytokeratin 17 as an immunohistochemical marker for intramural cytotrophoblast in human first trimester uteroplacental arteries

Pröll

Blaschitz

Hartmann

et al. 1997

Cell and Tissue Research

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“…The nonadherent cells were then transferred onto 35-mm Petri dishes that had been precoated with laminin. The trophoblast preparations obtained in this manner routinely contain 80-90% trophoblast cells with characteristics of extravillous trophoblast [31,32].…”

Section: Isolation Of Cells From Primary Tissuesmentioning

confidence: 99%

Human Decidual Natural Killer Cells Express the Receptor for and Respond to the Cytokine Interleukin 151

Verma

Hiby

Loke

et al. 2000

Biology of Reproduction

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260

150

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The natural killer (NK) cells that are present in the uterine mucosa (decidua) during early pregnancy have a distinctive phenotype, CD56(bright) CD16(-). These cells have previously been shown to proliferate and be activated by interleukin (IL)-2. However, IL-2 is absent from the decidua and placenta, and we have therefore investigated whether IL-15 is present in the uterus and can act on decidual NK cells. Both IL-15 mRNA and protein were found in a variety of cells but particularly in decidual macrophages. IL-15 induced a proliferative response in decidual NK cells that was blocked by anti-IL-15 and was augmented by stem cell factor. The cytolytic activity of decidual NK cells against K562 was augmented. Interestingly, in contrast to IL-2, although activation with IL-15 resulted in some killing of JEG-3 choriocarcinoma cells, normal trophoblast cells remained resistant to lysis. These findings suggest that IL-15 is a candidate cytokine responsible for NK cell proliferation in vivo in the progesterone-dominated secretory endometrium and early decidua.

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“…All antibodies used for staining were resuspended in PBS/BSA. The proportion of trophoblast in the preparations was monitored by immunofluorescence staining with monoclonal antibody BC-1, a monoclonal antibody (mAb) directed to an epitope present only on EVT [23]. After incubation on ice for 30 min, the cells were washed twice in PBS/BSA and stained for 30 min with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Becton Dickinson) diluted 1 in 15.…”

Section: Immunofluorescent Staining and Flow Cytometrymentioning

confidence: 99%

“…The purity of the trophoblast preparations was greater than 90% when cytospins of the cells were stained with a panel of antibodies to identify trophoblast [23]. Purified trophoblast was plated onto 35-mm culture dishes precoated with fibronectin (Collaborative Biomedical Research Products, Bedford, MA) at 20 g/ml for 45 min.…”

Section: Isolation and Culture Of Trophoblast Cellsmentioning

confidence: 99%

Localization of Leukemia Inhibitory Factor and Its Receptor in Human Placenta Throughout Pregnancy1

Sharkey

King

Clark

et al. 1999

Biology of Reproduction

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124

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Mice in which the gene that encodes the receptor (R) for leukemia inhibitory factor (LIF) has been deleted show abnormal growth and development of the placenta. This indicates that LIF plays an important role in placental development. The expression of LIF-R and LIF was examined in human trophoblast and decidua using in situ hybridization and immunocytochemistry. LIF-R mRNA and immunoreactivity was localized in villous and extravillous trophoblast throughout pregnancy, and in endothelial cells of the fetal villi. Strong expression of mRNA encoding LIF was detected in decidual leukocytes, which are abundant at the implantation site. Extravillous trophoblast, which invades the maternal decidua, therefore expresses LIF-R as it moves past decidual leukocytes, which express LIF mRNA. The effect of LIF on cultured human trophoblast was examined in vitro. Recombinant human LIF had no effect on [3H]thymidine incorporation by purified extravillous trophoblast, nor on expression of integrins alpha1, alpha5, or beta1 by isolated trophoblast. These results identify fetal endothelial cells and all cells of the trophoblast lineage as targets for the action of LIF in human placenta. Although its effects on trophoblast are not yet clear, LIF appears to mediate interactions between maternal decidual leukocytes and invading trophoblast. LIF may also play a critical role in controlling angiogenesis in the placental villi, since human fetal endothelial cells express LIF-R, and mice lacking a functional LIF receptor gene show altered vascular development in the placenta.

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Evaluation of a Monoclonal Antibody, BC‐1, Which Identifies an Antigen Expressed on the Surface Membrane of Human Extravillous Trophoblast

Cited by 23 publications

References 6 publications

Cytokeratin 17 as an immunohistochemical marker for intramural cytotrophoblast in human first trimester uteroplacental arteries

Cytokeratin 17 as an immunohistochemical marker for intramural cytotrophoblast in human first trimester uteroplacental arteries

Human Decidual Natural Killer Cells Express the Receptor for and Respond to the Cytokine Interleukin 151

Localization of Leukemia Inhibitory Factor and Its Receptor in Human Placenta Throughout Pregnancy1

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