Evaluation of a Modified Roche Enzymatic Ammonia Method for Roche Cobas 6000 (c501 Module) Automated Platform: When 5 µL Improves Performance

Vassena, Christian; Casati, Marco; Sala, Guillem; Perlangeli, Vittoria; Montagnese, Sara; Ippolito, Silvia; Carati, Lucia

doi:10.7754/clin.lab.2016.160336

Cited by 2 publications

(2 citation statements)

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“…Our laboratory has been experiencing high frequencies of sample error flags while using Roche method on the Cobas c502 analyzer. Previously, two other laboratories have reported similar problems [7], [8]. Seiden-Long et al reduced absorbance error rate by using the Randox reagent as an alternative to the Roche reagent.…”

Section: Discussionmentioning

confidence: 88%

Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform

Kaplon

Groot

Straalen

et al. 2019

Practical Laboratory Medicine

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IntroductionAmmonia is a metabolite of protein catabolism that, when elevated, may be toxic for tissues, especially for the central nervous system. Elevated ammonia in blood is an indicator and a prognostic factor for hepatic and kidney disease or inherited metabolic disorders in nitrogen metabolism. The accuracy of ammonia determination is influenced by sampling condition, handling, storage and assay itself. Our and other laboratories have been experiencing high frequencies sample error flags while measuring ammonia with glutamate dehydrogenase method on Roche Cobas 8000 platform. To reduce the number of error flags we adapted Roche NH3L protocol by incorporation of an additional onboard routine step for sample pre-dilution.Material and methodsThe AMC NH3L is an adaptation of Roche protocol that uses four fold pre-dilution of the sample in the rerun prior to the analysis. It was assessed for 1.occurrence of absorbance error flags, 2.precision, 3.correlation with Roche method and 4.interference by hemolysis, icterus and lipemia.ResultsThe AMC NH3L adaptation demonstrates acceptable within-run and total precision. Comparison studies show no differences between the Roche rerun application and AMC NH3L adaptation. The AMC NH3L adaptation solves 78% of absorbance errors and for samples with high ammonia concentration is less affected by interferences from icterus and hemolysis than the Roche rerun application.ConclusionThe AMC NH3L adaptation is less prone to instrument error flags and for samples with high ammonia concentration, is more robust to endogenous interferences. The AMC NH3L adaptation is viable alternative to the Roche protocol for the ammonia measurement.

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Section: Discussionmentioning

confidence: 88%

Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform

Kaplon

Groot

Straalen

et al. 2019

Practical Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…The studied salivary parameters (electrolytes, salivary enzymes, and substrate) were analyzed by an automated biochemistry analyzer (Cobas 6000, Roche Diagnostics, Mannheim, Germany) using human diagnostic kits (Roche Diagnostics, Mannheim, Germany). 9 Each measure was repeated three times, and the means were taken.…”

Section: Biochemical Parameter Measurementmentioning

confidence: 99%

Influence of orthodontic appliance type on salivary parameters during treatment

Dallel¹,

Salem²,

Merghni³

et al. 2020

The Angle Orthodontist

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Objectives To evaluate the effect of orthodontic appliances on physicochemical, biochemical, and oxidative stress changes in salivary parameters during treatment. Materials and Methods A cohort study was conducted with 112 healthy patients. Salivary samples were taken at baseline, 1 month, and 9 months after placement of the orthodontic appliances used in treatment. Results A statistically significant difference was observed in certain examined salivary parameters, including enzymes, electrolytes, and oxidative stress markers. Conclusions The use of aligners had a lower prevalence of disturbing salivary parameters. Orthodontist must consider these changes to prevent the occurrence of white spot lesions.

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Evaluation of a Modified Roche Enzymatic Ammonia Method for Roche Cobas 6000 (c501 Module) Automated Platform: When 5 µL Improves Performance

Cited by 2 publications

References 0 publications

Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform

Improved assay protocol for measurement of ammonia on the Roche Cobas 8000 automated platform

Influence of orthodontic appliance type on salivary parameters during treatment

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