Evaluation of a modified rat model for functional dyspepsia

Liang, Qian-Kun; Yuan, Yan; Mao, Lanfang; Du, Xiaojuan; Liang, Jingjing; Liu, Junhong; Wang, Longde; Li, Hongfang

doi:10.4103/sjg.sjg_505_17

Cited by 15 publications

(4 citation statements)

References 29 publications

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“…From day 22 onwards, feed pellets were introduced until the rat pups reached 7 weeks of age, and we commenced phase two of FD modeling. Phase two consisted of tail clamping to induce stress and delayed gastric emptying [ 11 , 26 ] and irregular feeding to disrupt regular feeding cycles [ 21 ] over a period of 14 days. Tail clamping provocation was implemented in the FD model and FD acupuncture groups by clamping sponge forceps at the distal 1/3 region of rat tails, without injury to the skin, for 30 minutes at a time, four times per day [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

Chen

Zhao

Tan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Functional Dyspepsia (FD) is a common functional gastrointestinal disease, which can reduce the quality of life in patients. Prior research has indicated that insula is closely related to FD and that acupuncture can regulate the functional connectivity (FC) of FD. Therefore, we hypothesized that acupuncture on FD was effected through the insular pathway. To test our hypothesis, we performed electroacupuncture (EA) on FD rat models and then examined the FC between insula and other brain regions through resting-state functional magnetic resonance imaging (rs-fMRI). Seven-day-old male infant Sprague-Dawley (SD) rats were randomly divided into control group, FD model group, and FD acupuncture group, with twelve rats per group (n = 36). Upon establishing successful models, the FD acupuncture group was subjected to EA intervention using Stomach back-shu (BL-21) and front-mu (RN-12) points for ten consecutive days for durations of 20 minutes each day. After intervention, each group was subject to rs-fMRI. The digital image data obtained were analyzed using FC analysis methods. Subsequently, gastric ligation was performed to measure gastric emptying rates. Before EA intervention, the FD model group exhibited decreased functional connections between the insula and a number of brain regions. After EA intervention, FD acupuncture group exhibited increasing FC between insula and regions when compared to the FD model group, such as the primary somatosensory cortex (S1), hippocampal CA3 (CA3), polymorphic layer of dentate gyrus (PoDG), caudate putamen (CPu), and oral pontine reticular nuclei (PnO) P < 0.05 ; decreasing FC was also exhibited between insula and regions such as the bilateral primary and secondary motor cortexes (M1/2), paraventricular hypothalamic nucleus (PVA), and limbic cortex (LC). These findings indicate that the effective treatment of FD using EA may be through regulating the abnormal FC between insula and several brain regions, in particular CA3, PoDG, and PVA.

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Section: Methodsmentioning

confidence: 99%

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

Chen

Zhao

Tan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…The total clinical effectiveness rate was described in twenty-one studies (Wang et al, 2008;Fan, 2010;Yang et al, 2010;Zhou, 2010;Liang, 2011;He, 2012;Lu and Hong, 2012;Cai et al, 2014;Liao, 2014;Chen, 2015;Cheng and Zhu, 2015;Wang and Yang, 2016;Zhou et al, 2016;Sun, 2017;Xu et al, 2017;Zeng and Tang, 2017;Zhou et al, 2018;Feng, 2020;Wang, 2020;Jiang, 2022;Li et al, 2022). XSLJZD demonstrated a significantly higher clinical effectiveness rate compared to the control group (RR 1.27; 95% CI 1.21, 1.33; p < 0.00001), with low heterogeneity observed (p = 0.68, I 2 = 0%) (Figure 4).…”

Section: Primary Outcome: the Total Clinical Effectiveness Ratementioning

confidence: 99%

“…The potential publication bias of the twenty-one studies (Wang et al, 2008;Fan, 2010;Yang et al, 2010;Zhou, 2010;Liang, 2011;He, 2012;Lu and Hong, 2012;Cai et al, 2014;Liao, 2014;Chen, 2015;Cheng and Zhu, 2015;Wang and Yang, 2016;Zhou et al, 2016;Sun, 2017;Xu et al, 2017;Zeng and Tang, 2017;Zhou et al, 2018;Feng, FIGURE 2 Risk of bias graph. Risk of bias summary.…”

Section: Publication Biasmentioning

confidence: 99%

“…Four studies (Liang, 2011;Wang and Yang, 2016;Guo and Chen, 2017;Lv et al, 2017) have reported a significant improvement in gastric emptying rate among patients with FD following treatment. XSLJZD demonstrated a significantly higher gastric emptying rate compared to the control group (RR 1.35; 95% CI 0.10, 2.59; p = 0.03), although there was substantial heterogeneity observed (I 2 = 97%, p < 0.00001) (Figure 7).…”

Section: Improvement Of Gastric Emptying Ratementioning

confidence: 99%

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Efficacy and safety of Xiangsha liujunzi decoction for functional dyspepsia: a systematic review and meta-analysis

Wang,

Ding,

Yao

et al. 2024

Front. Pharmacol.

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BackgroundFunctional dyspepsia is a highly prevalent digestive disorder. The limited effectiveness of current pharmaceutical interventions necessitates the exploration of alternative therapeutic options for functional dyspepsia. Xiangsha liujunzi decoction, a well-known traditional Chinese medicine formulation, has been widely employed in the treatment of functional dyspepsia in China. Nevertheless, the effectiveness of Xiangsha liujunzi decoction in the treatment of functional dyspepsia remains uncertain.ObjectiveTo examine the effectiveness and safety of Xiangsha liujunzi decoction for treating functional dyspepsia.MethodsWe retrieved seven databases containing randomized controlled trials on functional dyspepsia published up until 31 July 2023. The quality of these studies was evaluated using the Cochrane Risk of Bias assessment tool. The analysis of data was performed using the software RevMan 5.4. The total clinical effectiveness rate was evaluated as the primary outcome. In addition, gastric emptying rate, symptom score and safety evaluation were evaluated as the secondary outcomes.ResultsThe meta-analysis included 23 studies, involving 2,101 individuals. Xiangsha liujunzi decoction demonstrated a significantly higher clinical effectiveness rate compared to the control group (RR 1.27; 95% CI 1.21, 1.33; p < 0.00001). Moreover, it exhibited superior gastric emptying rate and symptom score improvement compared to the control group. Nevertheless, no remarkable differences were detected in safety between Xiangsha liujunzi decoction and the control group (RR 0.67; 95% CI 0.16, 2.76; p = 0.58).ConclusionThe findings of this study suggest that Xiangsha liujunzi decoction exhibits effectiveness and no significant adverse events observed. However, because of the low quality of the enrolled studies, more high-quality and strict design randomized controlled trials are required in the future.

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Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition

Tang

Zhang

et al. 2019

Dig Dis Sci

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Background Gastrointestinal motility disorder is an important pathological basis for functional dyspepsia (FD). Epigastric ache and discomfort are the main symptoms of FD, and ghrelin deficiency is closely related to the occurrence and development of FD. While electroacupuncture (EA) alleviated the symptoms of FD patients and improved their quality of life, there is a lack of sufficient mechanistic evidence to support these beneficial effects. Methods An in vivo FD model was established in wild-type and mammalian target of rapamycin (mTOR) knockout (−/−) rats. FD rats were subjected to EA with or without mTOR agonists or inhibitors. Gastric emptying and intestinal propulsion were assessed, and pathological changes in the hypothalamus, gastric antrum, and small intestine were examined histologically. In addition, ghrelin expression and AMPK/TSC2/Rheb/mTOR activation were detected by quantitative reverse transcription polymerase chain reaction and western blot. Results EA alone or in combination with mTOR inhibitors improved gastrointestinal function in FD rats by increasing the rates of intestinal propulsion and gastric emptying, and pathological changes in the hypothalamus, gastric antrum, and small intestine were alleviated. This may be related to the significant upregulation of ghrelin expression and the effective activation of the AMPK/TSC2/Rheb/mTOR signaling pathway. Interestingly, EA also improved gastrointestinal function and ghrelin expression in mTOR (−/−) KO FD rats. Conclusion Altering the level of ghrelin by regulating AMPK/TSC2/Rheb-mediated mTOR inhibition is an important way through which EA treats FD. The complex EA-mediated regulatory mechanisms of the brain-gut axis still require further exploration.

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Evaluation of a modified rat model for functional dyspepsia

Cited by 15 publications

References 29 publications

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

Efficacy and safety of Xiangsha liujunzi decoction for functional dyspepsia: a systematic review and meta-analysis

Electroacupuncture Upregulated Ghrelin in Rats with Functional Dyspepsia via AMPK/TSC2/Rheb-Mediated mTOR Inhibition

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