Evaluation of a modified complement fixation test and an indirect hemagglutination test for the serodiagnosis of melioidosis in pigs

Abstract

A complement fixation test modified by the addition of porcine serum and an indirect hemagglutination test were used to detect antibodies to Pseudomonas pseudomallei in pigs. These tests together with cultural examinations were carried out with 250 pigs. The sensitivity and specificity values were 79.3 and 99.5% and 82.8 and 93.2% for the modified complement fixation and hemagglutination tests, respectively. When results from the combination of both tests were considered, the values were 86.2 and 92.8%, respectively.

Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison

“…To assess the infection status of the remaining goats in this flock, and given the limited availability of tools to diagnose melioidosis in animals, especially in goats [29,30], several serological tests were used in our study, including those for glanders [25,28,31,32], which is caused by B. mallei, a bacterium closely related to B. pseudomallei. Although the complement fixation test has previously been effective in diagnosing melioidosis in goats and pigs [33,34], the use of this test with a B. mallei antigen in our study did not yield positive results, possibly due to the antigen used or the fact that the rest of the animals did not show acute infection. However, we observed positive ELISA results in 4 to 6 animals using two different kits, with the same animals testing positive on both kits.…”

Case-control investigation on the risk factors of melioidosis in small ruminant farms in Peninsular Malaysia

“…The CFT was the preferred as screening test because of its high specificity in serodiagnosis of melioidosis (Thomas et al 1988). The sensitivity of the CFT in for diagnosis of melioidosis in animals was estimated to be between 79Á3 and 82Á4% while its specificity was between 99Á5 and 100%, which qualifies the test to be a good screening test (Thomas et al 1988(Thomas et al , 1990. On the other hand, the culture and identification method was the preferred confirmatory test because it remains the gold standard in the diagnosis of melioidosis Meumann et al 2012).…”

Burkholderia pseudomallei and Melioidosis: Be Aware in Temperate Area

“…It was suggested that subclinical infection may result in cardiac conduction abnormalities and stress on the already compromised heart may cause sudden death. (133). It has been described, however, that CF or IHA titer does not reflect the stage of infection nor is it a measure of the disease activity (8).…”