Background: Early antibiotic treatment is a key component of therapy for sepsis. However, current blood culture can’t fulfill it. The aim of this study was to assess whether satellite blood cultures (immediate incubation of blood cultures in the ICU) can improve turnaround times, antibiotic switching and patient prognosis. Methods: This a observational single-center study, satellite blood culture and medical microbiology laboratory blood culture were performed separately in two groups. septic patients are collected from department of critical care medicine, Henan Provincial People`s Hospital from February 5,2018, to January 19, 2019.All septic patients are older than 18 and diagnosed sepsis according sepsis-3 criteria, who have been stayed hospital stay longer than 48 hour admitted to ICU. Results: In total, we analyzed 299 blood culture sets from 73 ICU patients, including:149 from the medical microbiology laboratory blood culture group and 150 from the satellite blood culture group. There is no significant difference between the two group in positive rate. Comparing to laboratory blood culture group, the median time from specimen collection until incubation as reduced by 3.02h in satellite blood culture group (P < 0.001); the median time from specimen collection to notify blood culture positivity to the clinicians was reduced by 9.76h in satellite blood culture group(P <0.001).The median time was 54.05h in medical microbiology laboratory blood culture group and 30.97h in satellite blood culture group until the first change of antibiotic therapy regimen (P = 0.056).There was no difference in duration of ICU stay、duration of hospital stay、medical cost between the two group. Conclusion: We have found that satellite blood culture may reduced turnaround times and it may speed up antibiotic switching and benefit on patients outcomes.