Evaluation of a method for monoclonal antibody HLA‐B27 analysis with the CELL‐DYN Sapphire haematology analyser

Aarsand, Aasne K.; Johannessen, Helene; Scott, Colin

doi:10.1111/j.1751-553x.2007.00912.x

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…These results are in line with studies from the field of clinical chemistry, showing that results from the CD-Sapphire were comparable to their conventional flow cytometry in diagnostic measurements [13, 15, 18]. In addition, the machine has a fully automated modus for staining and incubating whole blood samples with antibodies, lysis of erythrocytes, and subsequent data acquisition [14].…”

Section: Discussionsupporting

confidence: 81%

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Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser

Groeneveld

Heeres

Leenen

et al. 2012

Mediators of Inflammation

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Introduction. Flow cytometry markers have been proposed as useful predictors for the occurrence of posttraumatic inflammatory complications. However, currently the need for a dedicated laboratory and the labour-intensive analytical procedures make these markers less suitable for clinical practice. We tested an approach to overcome these limitations. Material and Methods. Neutrophils of healthy donors were incubated with antibodies commonly used in trauma research: CD11b (MAC-1), L-selectin (CD62L), FcγRIII (CD16), and FcγRII (CD32) in active form (MoPhab A27). Flow cytometric analysis was performed both on a FACSCalibur, a standard flow cytometer, and on a Cell-Dyn Sapphire, a routine haematology analyser. Results. There was a high level of agreement between the two types of analysers, with 41% for FcγRIII, 80% for L-selectin, 98% for CD11b, and even a 100% agreement for active FcγRII. Moreover, analysis on the routine haematology analyser was possible in less than a quarter of the time in comparison to the flow cytometer. Conclusion. Analysis of neutrophil phenotype on the Cell-Dyn Sapphire leads to the same conclusion compared to a standard flow cytometer. The markedly reduced time necessary for analysis and reduced labour intensity constitutes a step forward in implementation of this type of analysis in clinical diagnostics in trauma research.

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Section: Discussionsupporting

confidence: 81%

“…One of these machines, the CELL-DYN Sapphire (CD-Sapphire), also offers fluorescent flow cytometry capacities. Compared to a conventional flow cytometer, the CD-Sapphire could provide a greater degree of automation and could thus be operated with relatively little training [13]. …”

Section: Introductionmentioning

confidence: 99%

Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser

Groeneveld

Heeres

Leenen

et al. 2012

Mediators of Inflammation

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Comparative study of two laboratory techniques for the detection of HLA-B27 in patients with axial spondyloarthritis: a cross-sectional analysis

dos Santos Angeli,

Ribeiro,

Kohem

et al. 2024

Adv Rheumatol

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Background The diagnostic and prognostic relevance of Human Leukocyte Antigen B-27 (HLA-B27) in Axial Spondyloarthritis (AxSpA) is undeniable, with 70% of Ankylosing Spondylitis (AS) patients carrying the B27 gene, contrasted with a mere 4.35% in the general population. Flow cytometry (FC) and Polymerase Chain Reaction (PCR) have emerged as the predominant techniques for routine HLA-B27 typing. While various studies have compared these methods, none have catered to the unique characteristics of the Brazilian demographic. Therefore, this research aims to compare FC and PCR in a Brazilian cohort diagnosed with AxSpA. Methods An analytical cross-sectional study was undertaken involving 62 AxSpA outpatients from a Brazilian University Hospital. Both FC and PCR-SSP assays were utilized to ascertain HLA-B27 typing. The outcomes (either confirming or refuting the allele’s presence) underwent rigorous scrutiny. Agreement between the methodologies was assessed using the kappa statistic. A p-value of < 0.05 was deemed statistically significant. Results Of the participants, 90.3% (n = 56) were HLA-B27 positive according to FC, while 79% (n = 49) were identified as positive using the PCR method. FC exhibited a sensitivity rate of 98% paired with a specificity of 38.5%. The Positive Predictive Value for FC stood at 85.7%, and the Negative Predictive Value was 83.5%. Consequently, the overall accuracy of the FC method was gauged at 85.5%. A kappa coefficient of κ = 0.454 was derived. Conclusions FC demonstrated noteworthy sensitivity and satisfactory accuracy in HLA-B27 detection, albeit with a reduced specificity when contrasted with PCR-SSP. Nevertheless, given its cost-effectiveness and streamlined operation relative to PCR, FC remains a pragmatic option for preliminary screening in clinical practice, especially in low-income regions. To optimize resource allocation, we advocate for a refined algorithm that initiates by assessing the relevance of HLA-B27 typing based on Choosing Wisely recommendations. It then leans on FC, and, if results are negative yet clinical suspicion persists, advances to PCR. This approach aims to balance diagnostic accuracy and financial prudence, particularly in regions contending with escalating medical costs.

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Evaluation of a method for monoclonal antibody HLA‐B27 analysis with the CELL‐DYN Sapphire haematology analyser

Cited by 2 publications

References 9 publications

Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser

Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser

Comparative study of two laboratory techniques for the detection of HLA-B27 in patients with axial spondyloarthritis: a cross-sectional analysis

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