Evaluation of a lipopeptide biosurfactant from Bacillus natto TK-1 as a potential source of anti-adhesive, antimicrobial and antitumor activities

Cao, Xiaohong; Liao, Zhenyu; Wang, Chunling; Yang, Wenyan; Lu, Ming–Wei

doi:10.1590/s1517-83822009000200030

Cited by 93 publications

(48 citation statements)

References 26 publications

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“…Biosurfactants from three tested B. subtilis strains gave positive oil spread test, showing the diameter of oil displacement from 3 to 7 mm. These results are in accordance with data for crude biosurfactants from B. subtilis natto [21] and from B. subtilis and B. licheniformis [33]. Inhibition activity of biosurfactants was found only against Staphylococcus strains and depended on the incubation temperature of Bacillus culture.…”

Section: Discussionsupporting

confidence: 90%

“…These compounds vary in structure and spectrum of activity and usually are responsible for antimicrobial effects of Bacillus bacteria [21,32]. In our study B. subtilis strains produced biosurfactants after cultivation in starch broth at 30°C.…”

Section: Discussionmentioning

confidence: 90%

“…In our study B. subtilis strains produced biosurfactants after cultivation in starch broth at 30°C. Incubation of these cultures in fermentation medium, used for B. subtilis natto [21], resulted in lack of biosurfactants production. Presence of biosurfactant in cultivation medium was tested by the oil spread test and by inhibition activity against Salmonella, Shigella and Staphylococcus strains.…”

Section: Discussionmentioning

confidence: 99%

“…Seed cultures were inoculated into nutrition media (1 mL of overnight culture into 250-mL flack with 50 mL of tested medium). Two media were used for cultures cultivation: SA and a fermentation media for biosurfactant production by B. subtilis natto, composed [21]. Bacillus strains were incubated 24 hours at 30° and 37°C.…”

Section: Biosurfactant Evaluationmentioning

confidence: 99%

“…Biosurfactant production was tested in two media-starch broth and fermentation medium, used for surfactant production by B. subtilis natto [21]. B. subtilis strains were incubated in two medium at 30°C and 37°C.…”

Section: Biosurfactant Productionmentioning

confidence: 99%

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Antagonistic Activity of Bacillus Bacteria against Food-Borne Pathogens

Moore¹

2013

J Prob Health

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Bacillus bacteria have attracted the attention of scientists as promising probiotics because of their versatile antimicrobial activity and established health benefits on the host. In this study, seven Bacillus strains were identified and analyzed for antagonistic activity against broad spectrum of food borne pathogens. All strains were identified as B. subtilis, based on the results of morphological, biochemical characterization and 16S rDNA sequence analysis. B. subtilis strains demonstrated antagonistic activity against test-cultures of pathogens, including multiresistant strains. Reference Bacillus strains, derived from the commercial probiotics did not show antagonistic activity against tested strains of pathogens. Three the most active cultures were studied for production of biosurfactants. Crude biosurfactants were isolated and analyzed by oil spread test and inhibition activity against Salmonella, Shigella and Staphylococcus cultures. Biosurfactants from three tested B. subtilis strains gave positive oil spread test. Inhibition activity of biosurfactants was found only against Staphylococcus strains. Production of biosurfactants depended on the incubation conditions of Bacillus culture. Best results were obtained after cultivation of bacilli in starch broth at 30°C. The concentration of produced biosurfactant increased in time with growth of bacteria and reached the maximum at 30 hours of incubation.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Biosurfactant Evaluationmentioning

confidence: 99%

Section: Biosurfactant Productionmentioning

confidence: 99%

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Antagonistic Activity of Bacillus Bacteria against Food-Borne Pathogens

Moore¹

2013

J Prob Health

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Review lipopeptides biosurfactants: Mean classes and new insights for industrial, biomedical, and environmental applications

2015

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Lipopeptides are microbial surface active compounds produced by a wide variety of bacteria, fungi, and yeast. They are characterized by high structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface, respectively. Surfactin, iturin, and fengycin of Bacillus subtilis are among the most popular lipopeptides. Lipopepetides can be applied in diverse domains as food and cosmetic industries for their emulsification/de-emulsification capacity, dispersing, foaming, moisturizing, and dispersing properties. Also, they are qualified as viscosity reducers, hydrocarbon solubilizing and mobilizing agents, and metal sequestering candidates for application in environment and bioremediation. Moreover, their ability to form pores and destabilize biological membrane permits their use as antimicrobial, hemolytic, antiviral, antitumor, and insecticide agents. Furthermore, lipopeptides can act at the surface and can modulate enzymes activity permitting the enhancement of the activity of certain enzymes ameliorating microbial process or the inhibition of certain other enzymes permitting their use as antifungal agents. This article will present a detailed classification of lipopeptides biosurfactant along with their producing strain and biological activities and will discuss their functional properties and related applications.

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Identification and natural functions of cyclic lipopeptides from Bacillus amyloliquefaciens An6

Ayed

Hmidet

Béchet

et al. 2016

Engineering in Life Sciences

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Lipopeptides constitute a structurally diverse group of metabolites produced by various bacterial and fungal genera. In the past decades, research on lipopeptides has been fueled by their surfactant activities. However, natural functions of lipopeptides compounds have received considerably less attention. The aim of this study was to isolate and identify the lipopeptides from Bacillus amyloliquefaciens An6, and further evaluate their biological activities. An6 lipopeptides were detected by PCR using degenerated primers and MALDI‐TOF‐MS. An6 strain was found to produce surfactin, fengycin, and bacillomycin. Following their purification, the in vitro antioxidant activity of An6 lipopeptides was studied through different assays. The scavenging effect on 1,1‐diphenyl‐2‐picrylhydrazyl radicals at a dosage of 0.75 mg/mL was 81%. Its reducing power was concentration‐dependant and reached a maximum of 1.07 at 2.5 mg/mL. Moreover, they showed a strong inhibition of β‐carotene bleaching. An6 lipopeptides mixture was also found to display significant antimicrobial activity against several Gram‐positive, Gram‐negative bacteria, and fungal strains. An6 lipopeptides were insensitive to proteolytic enzymes, stable between pH 4.0 and 12.0, and resistant to high temperature. Our results provided enough evidence proving that An6 lipopeptides could be used as functional‐food components.

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Evaluation of a lipopeptide biosurfactant from Bacillus natto TK-1 as a potential source of anti-adhesive, antimicrobial and antitumor activities

Cited by 93 publications

References 26 publications

Antagonistic Activity of Bacillus Bacteria against Food-Borne Pathogens

Antagonistic Activity of Bacillus Bacteria against Food-Borne Pathogens

Review lipopeptides biosurfactants: Mean classes and new insights for industrial, biomedical, and environmental applications

Identification and natural functions of cyclic lipopeptides from Bacillus amyloliquefaciens An6

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