Evaluation of a Latex Agglutination Test (MRSA-Screen) for Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci

Louie, Lisa; Majury, Anna; Goodfellow, Janet; Louie, Marie; Simor, Andrew E.

doi:10.1128/jcm.39.11.4149-4151.2001

Cited by 45 publications

(48 citation statements)

References 16 publications

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“…Moreover, in all isolates with the mecA gene, the presence of its PBP2a product was detected. This finding correlates with the data presented by Louie et al (2001); in their study, all isolates with genetically determined methicillin resistance showed agglutination indicating the presence of PBP2a (84 mecA-positive/84 PBP2a-positive isolates). Likewise, Miller et al (2005) demonstrated 100% agreement between the presence of the mecA gene and the modified protein of low affinity to penicillin.…”

Section: Discussionsupporting

confidence: 80%

Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

Bochniarz¹,

Wawron²,

Szczubiał³

2013

Polish Journal of Veterinary Sciences

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The aim of this study was to determine the mechanisms of staphylococcal resistance to methicillin. CNS (n=100 isolates) were prepared from the mammary inflammatory secretions of 86 cows from farms located in the Lublin region.Methicillin-resistant isolates constituted 20.0% of all CNS. Staphylococcus sciuri (n=8) and Staphylococcus xylosus (n=6) were most abundant, followed by Staphylococcus chromogenes (n=3), Staphylococcus haemolyticus (n=2) and Staphylococcus warneri (n=1). The mecA gene was found in 50.0% of MRCNS (10.0% of all CNS isolates) belonging to two species: S. sciuri and S. xylosus. All mecA-positive isolates contained the protein of low affinity to penicillin (penicillin-binding protein 2a -PBP2a). The enzyme hydrolysing the β-lactam ring in antibiotics was detected in 40.0% of MRCNS; 10.0% of MRCNS isolates were characterised by the presence of the mecA gene and ability to produce β-lactamase. The remaining 20.0% of MRCNS isolates showing phenotypic resistance to methicillin were mecA gene-negative and were not able to produce β-lactamase.

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Section: Discussionsupporting

confidence: 80%

Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

Bochniarz¹,

Wawron²,

Szczubiał³

2013

Polish Journal of Veterinary Sciences

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“…We only found major errors (falseresistance) with the oxacillin disk in 2.5% of the isolates, including S. sciuri, S. saprophyticus and S. cohnii-cohnii. Similar discrepancies between phenotypic and genotypic results have been described for S. saprophyticus, S. cohnii, S. warneri, S. capitis, S. lugdunensis and S. xylosus [32].…”

Section: Discussionmentioning

confidence: 60%

“…Total concordance between the latex agglutination test for PBP2a and mecA gene detection by PCR has been observed in isolates that showed false resistance with oxacillin disk diffusion or dilution. The PBP2a test was performed with and without induction, and identical results were observed with the different methods [32,36]. When we analyzed the false positive results obtained with agar dilution (Table 2), we found that eight isolates gave MICs of 0.5 µg/mL (low level of resistance or borderline resistance).…”

Section: Discussionmentioning

confidence: 96%

“…This can be explained by the fact that cefoxitin has a strong ability to induce PBP2a and also has a high affinity for PBP4, a protein involved in resistance in Staphylococcus spp. These organisms, although less frequent in the laboratory routine, could be more affected by the lower specificity of the oxacillin disk diffusion test and/or the oxacillin agar dilution test [32,34]. According to NCCLS 2001, the disk diffusion test with oxacillin is not recommended for S. saprophyticus, since most isolates that are mecA gene negative can express resistance based on phenotypic methods [35].…”

Section: Discussionmentioning

confidence: 99%

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Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci

Secchi¹,

Antunes²,

Perez³

et al. 2008

Braz J Infect Dis

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The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µ µ µ µ µg) with oxacillin disks (1 µ µ µ µ µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin gave a specificity of 88%. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillinresistance in staphylococci.

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“…However, several studies reported that this technique is sensitive and can be used as a confirmatory test for the results of disk diffusion (Hussain et al, 1998;Cavassini et al, 1999;Louie et al, 2001). Ferreira et al (2003) found that the screening test with 4 µg/ml oxacillin showed 100% sensitivity and 100% specificity versus 94.2% sensitivity and 100% specificity when a concentration of 6 µg/ml had been used, indicating the former as a screening method for CoNS.…”

Section: Discussionmentioning

confidence: 99%

Methods for Detection of Oxacillin Resistance among Coagulase-Negative Staphylococci Recovered from Patients with Bloodstream Infections at the University Hospital in Brazil

Ustulin¹,

Cunha²

2012

JVM

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Coagulase-negative staphylococci (CoNS) are among the main causative agents of nosocomial infections, and multi-resistant strains has become a major issue over the past decades. The objectives of this study are to determine the frequency of oxacillin resistance among CoNS strains isolated from blood cultures of patients admitted at the University Hospital of the Faculty of Medicine of Botucatu, Brazil, and to compare phenotypic methods with the gold standard (amplification of the mecA gene) used for the detection of methicillin resistant-CoNS. Among the 103 CoNS strains analyzed, 85 were identified as S. epidermidis, ten as S. warneri, five as S. haemolyticus, one as S. capitis, one as S. hominis and one as S. lugdunensis. Amplification of the mecA gene revealed 85 (82.5%) positive strains. Of these, 81 (95.3%) were resistant by the oxacillin and cefoxitin disk diffusion method, 82 (96.5%) by screening on Mueller-Hinton agar supplemented with 4 µg/ml oxacillin and 4% NaCl and 84 (98.8%) by the E-test. The mecA gene was detected in 82.3% of the S. epidermidis isolates and in seven of the ten S. warneri isolates. Among the phenotypic methods studied, the E-test yielded the best results when compared to the gold standard. The results showed that most of the CoNS strains were resistant to oxacillin and to multiple drugs; a fact making treatment of infections caused by these microorganisms more difficult.

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Evaluation of a Latex Agglutination Test (MRSA-Screen) for Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci

Cited by 45 publications

References 16 publications

Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

Resistance to methicillin of coagulase-negative staphylococci (CNS) isolated from bovine mastitis

Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci

Methods for Detection of Oxacillin Resistance among Coagulase-Negative Staphylococci Recovered from Patients with Bloodstream Infections at the University Hospital in Brazil

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