Evaluation of a Labelled Bacteriophage with 99mTc as a Potential Agent for Infection Diagnosis

Cardoso, María Elena; Fernández, Leticia; Tejería, Emilia; Esperón, Patricia; Terán, Mariella

doi:10.2174/1874471009666160215162023

Cited by 16 publications

(12 citation statements)

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“…After only 5 min, 17% of the injected phages were present in the urinary bladder. After 30 min and after 3 h, such a fraction was as high as 30% and >50%, respectively [201,202]. The level of phage excretion thorough the urinary tract is sufficient to achieve a therapeutic effect in the treatment of UTIs by the oral administration of phage preparations.…”

Section: Bacteriophages In Urinary Tractmentioning

confidence: 98%

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Podlacha

Grabowski

Kosznik-Kawśnicka

et al. 2021

IJMS

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Bacteriophages are viruses infecting bacterial cells. Since there is a lack of specific receptors for bacteriophages on eukaryotic cells, these viruses were for a long time considered to be neutral to animals and humans. However, studies of recent years provided clear evidence that bacteriophages can interact with eukaryotic cells, significantly influencing the functions of tissues, organs, and systems of mammals, including humans. In this review article, we summarize and discuss recent discoveries in the field of interactions of phages with animal and human organisms. Possibilities of penetration of bacteriophages into eukaryotic cells, tissues, and organs are discussed, and evidence of the effects of phages on functions of the immune system, respiratory system, central nervous system, gastrointestinal system, urinary tract, and reproductive system are presented and discussed. Modulations of cancer cells by bacteriophages are indicated. Direct and indirect effects of virulent and temperate phages are discussed. We conclude that interactions of bacteriophages with animal and human organisms are robust, and they must be taken under consideration when using these viruses in medicine, especially in phage therapy, and in biotechnological applications.

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Section: Bacteriophages In Urinary Tractmentioning

confidence: 98%

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Podlacha

Grabowski

Kosznik-Kawśnicka

et al. 2021

IJMS

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“…evaluated another 99m Tc-labeled P. aeruginosa -specific bacteriophage (PP7), describing a 1.68 times higher signal in the infected side compared with sterile inflammation. 171 In these studies, the authors describe positive signal from the gastrointestinal tract, suggesting that the bacteriophages are binding to the host microbiome.…”

Section: Bacteriophagesmentioning

confidence: 99%

Pathogen-Specific Bacterial Imaging in Nuclear Medicine

Ordoñez

Jain

2018

Seminars in Nuclear Medicine

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When serious infections are suspected, patients are often treated empirically with broad-spectrum antibiotics while awaiting results that provide information on the bacterial class and species causing the infection, as well as drug susceptibilities. For deep-seated infections, these traditional diagnostic techniques often rely on tissue biopsies to obtain clinical samples which can be expensive, dangerous, and has the potential of sampling bias. Moreover, these procedures and results can take several days and may not always provide reliable information. This combination of time and effort required for proper antibiotic selection has become a barrier leading to indiscriminate broad-spectrum antibiotic use. Exposure to nosocomial infections and indiscriminate use of broad-spectrum antibiotics are responsible for promoting bacterial drug-resistance leading to substantial morbidity and mortality, especially in hospitalized and immunosuppressed patients. Therefore, early diagnosis of infection and targeted antibiotic treatments are urgently needed to reduce morbidity and mortality caused by bacterial infections worldwide. Reliable pathogen-specific bacterial imaging techniques have the potential to provide early diagnosis and guide antibiotic treatments.

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“…5). More recently, Cardoso et al [87] evaluated in a biodistribution study the 99m Tc-labelled bacteriophage PP7 for detecting P. aeruginosa infections in mice and this tracer had an increased accumulation in P. aeruginosa-infected tissues (abscess-to-muscle ratio 4.2 ± 0.3) compared to sterile inflammations (abscess-to-muscle ratio 2.5 ± 0.4).…”

Section: Imaging Of Infections With Radiolabelled Bacteriophagesmentioning

confidence: 99%

An update on radiotracer development for molecular imaging of bacterial infections

Welling

Hensbergen

Bunschoten

et al. 2019

Clin Transl Imaging

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Background Bacterial infections are still a major global healthcare problem. To combat the increasing antimicrobial resistance, early diagnosis of bacterial infections-including the identification of bacterial species-is needed to improve antibiotic stewardship and to help reduce the use of broad-spectrum antibiotics. To aid successful targeted antibiotic treatment, specific detection and localisation of infectious organisms is warranted. Nuclear medicine imaging approaches have been successfully used to diagnose bacterial infections and to differentiate between pathogen induced infections and sterile inflammatory processes. Aim In this comprehensive review we present an overview of recent developments in radiolabelled bacterial imaging tracers. Methods The PubMed/MEDLINE and Embase (OvidSP) literature databases were systematically searched for publications on SPECT and PET on specific imaging of bacterial using specific guidelines with MeSH-terms, truncations, and completion using cross-references. Tracers in literature that was extensively reviewed before 2016 were not included in this update. Where possible, the chemical structure of the radiolabelled compounds and clinical images were shown. Results In 219 original articles pre-clinical and clinical imaging of bacterial infection with new tracers were included. In our view, the highest translational potential lies with tracers that are specific to target the pathogens: e.g., 99m Tc-and 68 Galabelled UBI 29-41 , 99m Tc-vancomycin, m-[ 18 F]-fluoro-PABA, [methyl-11 C]-D-methionine, [ 18 F]-FDS, [ 18 F]-maltohexaose and [ 18 F]-maltotriose. An encouraging note is that some of these tracers have already been successfully evaluated in clinical settings. Conclusion This review summarises updates in tracer development for specific (pre-clinical and clinical) imaging of bacterial infections. We propsed some promising tracers that are likely to become innovative standards in the clinical setting in the near feature.

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Evaluation of a Labelled Bacteriophage with 99mTc as a Potential Agent for Infection Diagnosis

Abstract: Targeted biodistribution profile and good T/NT ratios, indicate that this complex presents enough specificity to discriminate between infection caused by Pseudomonas aeruginosa and sterile inflammation.

Cited by 16 publications

References 0 publications

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Pathogen-Specific Bacterial Imaging in Nuclear Medicine

An update on radiotracer development for molecular imaging of bacterial infections

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