Evaluation of a fluorogenic real-time reverse transcription-polymerase chain reaction method for the specific detection of all known serotypes of porcine teschoviruses

Cano-Gómez, Cristina; Buitrago, Dolores; Fernández-Piñero, Jovita; Fernández‐Pacheco, Paloma; Mansilla, Carmen; Agüero, Montserrat; Jiménez‐Clavero, Miguel Ángel

doi:10.1016/j.jviromet.2011.05.035

Cited by 10 publications

(3 citation statements)

References 19 publications

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“…Real-time PCR was conducted by the DTU National Veterinary Institute (Copenhagen, Denmark). No Porcine teschovirus (PTV) was detected in pulmonary samples from the three litters by real-time PCR [ 29 ]. This analysis was performed by the Instituto Nacional de Investigación y Technología Agraria y Alimentaria (INIA, Madrid, Spain).…”

Section: Case Presentationmentioning

confidence: 99%

Porcine circovirus type 2 associated reproductive failure in a specific pathogen free (SPF) piglet producing herd in Norway: a case report

Oropeza-Moe

Delgado²,

Framstad

2017

Porc Health Manag

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BackgroundSince 1999, scientists have published evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure in pigs. Affected herds have frequently been start-up herds, either naïve or with a high proportion of PCV2 susceptible gilts. Here, delayed farrowing in non-vaccinated gilts was observed in a commercial specific pathogen free (SPF) herd. Mummified fetuses and stillborn piglets recovered from these gilts were PCV2 positive.Case presentationThe case herd was a self-recruiting, piglet producing unit of 240 sows. After detecting livestock associated methicillin resistant Staphylococcus aureus (LA-MRSA, CC398), stamping out was imposed by the authorities. An SPF herd was re-established and all dams were vaccinated against PCV2 until the farmer decided to exclude this vaccine. The first non-vaccinated batch consisted of 76% gilts. Here, one gilt showed signs of impending farrowing. This gilt was slaughtered three to four weeks after the expected farrowing date without having expelled any uterine contents. In the subsequent batch consisting of 79% gilts, three gilts showed similar clinical signs. Delayed farrowing was observed in two of these gilts and the uterine contents from the third gilt were recovered at the abattoir. Mummified fetuses and stillborn piglets were recovered from all three gilts. High levels of PCV2 DNA (>107 viral genomic copies/ 500 ng tissue) were found in myocardial samples by real-time PCR analysis. One myocardial sample submitted for immunohistochemical (IHC) analysis showed moderate amounts of PCV2 antigen. In the subsequent batch consisting of 77% gilts, several weak-born piglets were seen across different litters.ConclusionsThis case report describes an apparent link between in utero PCV2 infection, pre partum nest-building behaviour, mammary development and delayed farrowing. To date, no reports have described imminent signs of farrowing and delayed farrowing as clinical signs in conjunction with transplacental PCV2 infection in Norway. Reinitiation of PCV2 vaccination was strongly advised in this herd due to recent depopulation and repopulation and the high proportion of gilts. Vaccination was effective because no further cases have occurred since.

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Section: Case Presentationmentioning

confidence: 99%

Porcine circovirus type 2 associated reproductive failure in a specific pathogen free (SPF) piglet producing herd in Norway: a case report

Oropeza-Moe

Delgado²,

Framstad

2017

Porc Health Manag

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“…There are several PCR-based assays available for the specific singleplex (e.g., PTV: [ 23 , 24 ]; PSV: [ 25 ]; PoAstV-3: [ 26 ]) or duplex (e.g., PSV&PTV: [ 27 ]) detection of these viruses, but an assay for the simultaneous detection of all three viruses is currently unknown.…”

Section: Introductionmentioning

confidence: 99%

Development and Large-Scale Testing of a Novel One-Step Triplex RT-qPCR Assay for Simultaneous Detection of “Neurotropic” Porcine Sapeloviruses, Teschoviruses (Picornaviridae) and Type 3 Porcine Astroviruses (Astroviridae) in Various Samples including Nasal Swabs

László

Pankovics

Reuter

et al. 2022

Viruses

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Porcine sapeloviruses, teschoviruses of family Picornaviridae and type 3 porcine astroviruses of family Astroviridae are (re-)emerging enteric pathogens that could be associated with severe, disseminated infections in swine, affecting multiple organs including the central nervous system (CNS). Furthermore, small-scale pioneer studies indicate the presence of these viruses in porcine nasal samples to various extents. The laboratory diagnostics are predominantly based on the detection of the viral RNA from faecal and tissue samples using different nucleic-acid-based techniques such as RT-qPCR. In this study, a novel highly sensitive one-step triplex RT-qPCR assay was introduced which can detect all known types of neurotropic sapelo-, tescho- and type 3 astroviruses in multiple types of samples of swine. The assay was evaluated using in vitro synthesized RNA standards and a total of 142 archived RNA samples including known sapelo-, tescho- and type 3 astrovirus positive and negative CNS, enteric and nasal specimens. The results of a large-scale epidemiological investigation of these viruses on n = 473 nasal swab samples from n = 28 industrial-type swine farms in Hungary indicate that all three neurotropic viruses, especially type 3 astroviruses, are widespread and endemically present on most of the investigated farms.

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“…Die Diagnose einer Teschovirus bedingten Polioencephalomyelitis bedarf neben klinischen und pathomorphologischen Befunden der Virusisolation, beziehungsweise des Nachweises von viralem Antigen mittels Immunhistochemie oder von Genomfragmenten mittels PCR aus Proben des ZNS 1 , 21 , 22 . Die kostengünstige sowie material- und zeitsparende PCR, die zum Teil eine Differenzierung der PTV-Serotypen ermöglicht, wird in der Routinediagnostik bevorzugt angewendet 22 , 23 , 24 .…”

Section: Introductionunclassified

Hinterhandlähmungen bei Mastschweinen im Zusammenhang mit einem neuen Stamm des porzinen Teschovirus A11

Stadler

Junker

Gründl

et al. 2022

Tierarztl Prax Ausg G Grosstiere Nutztiere

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ZusammenfassungIn einem Schweinemastbetrieb in Süddeutschland konnten über einen Zeitraum von 4 Wochen Hinterhandlähmungen bei 2 Altersgruppen (50 kg sowie 60 kg) beobachtet werden. Die Morbidität bei den beiden Altersgruppen war mit 3,3 % niedrig, der Großteil der betroffenen Tiere musste jedoch aufgrund einer fortschreitenden Paralyse der Hintergliedmaßen euthanasiert werden. Im Rahmen der pathomorphologischen Untersuchung von 2 Mastschweinen konnte eine hochgradige und diffuse lymphohistiozytäre Meningoenzephalomyelitis und Vaskulitis festgestellt werden. Immunhistochemisch wurde in allen Arealen des zentralen Nervensystems und in mehreren Zelltypen (Neuronen, Gliazellen, Endothelzellen, mononukleäre Zellen) Porzines Teschovirus-Antigen nachgewiesen. Die Untersuchung von Rückenmarksproben mittels PCR ergab den Nachweis von Porzinen Teschoviren. Der anschließend durchgeführten phylogenetischen Analyse zufolge wiesen die PCR positiven Proben die höchste Identität (88 % Vollgenomsequenz) zum Porzinen Teschovirus A11 Stamm „Dresden“ auf. Andere differentialdiagnostisch in Frage kommende Pathogene konnten mittels PCR, bakteriologischer Untersuchung und Sequenzierung nicht nachgewiesen werden. In dem Mastbestand traten nach ca. 4 Wochen keine weiteren Fälle von Lähmungserscheinungen mehr auf.

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Evaluation of a fluorogenic real-time reverse transcription-polymerase chain reaction method for the specific detection of all known serotypes of porcine teschoviruses

Cited by 10 publications

References 19 publications

Porcine circovirus type 2 associated reproductive failure in a specific pathogen free (SPF) piglet producing herd in Norway: a case report

Porcine circovirus type 2 associated reproductive failure in a specific pathogen free (SPF) piglet producing herd in Norway: a case report

Development and Large-Scale Testing of a Novel One-Step Triplex RT-qPCR Assay for Simultaneous Detection of “Neurotropic” Porcine Sapeloviruses, Teschoviruses (Picornaviridae) and Type 3 Porcine Astroviruses (Astroviridae) in Various Samples including Nasal Swabs

Hinterhandlähmungen bei Mastschweinen im Zusammenhang mit einem neuen Stamm des porzinen Teschovirus A11

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