Evaluation of a Dual Radioisotope Urinary Excretion Test in the Diagnosis of Pernicious Anaemia

Bell, T. H.; Lee, D

doi:10.1159/000208778

Cited by 10 publications

(3 citation statements)

References 3 publications

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“…The bound : free ratio of plasma radioactivity did not exceed 1.6 in any normal subject, and this has, therefore, been accepted as the upper Measurement of plasma radioactivity in vitamin B12 absorption stud ies is well documented [4,6] and the diagnostic value of this test has be come established in recent years. Bell et al [2] and B ell and L ee [3] described a test, based on the method of K atz et al [9], which com bined the two stages of the urinary excretion (Schilling) test. The basis of this test is the simultaneous administration of radioactive Co-labelled vitamin B I2 in 2 isotopic forms, one of which is 'free' while the other is 'bound' to normal human gastric juice.…”

Section: Resultsmentioning

confidence: 99%

Plasma Radioactivity Following Simultaneous Oral Administration of Intrinsic Factor-Bound and Free Radioactive Vitamin B<sub>12</sub>

Finney¹,

Payne²

1972

Acta Haematol

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Plasma radioactivity following the simultaneous oral administration of vitamin B₁₂ labelled with 2 test isotopes of cobalt (‘Dicopac’ test) was measured in 19 control subjects and in 19 patients suffering from pernicious anaemia. One isotope (⁵⁸Co) was free, while the other (⁵⁷Co) was bound to normal human gastric juice. Results of diagnostic value were obtained on 80% of occasions; misleading results occurred in the other 20%. It is concluded that this test is of little value in routine diagnostic haematology.

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Section: Resultsmentioning

confidence: 99%

Plasma Radioactivity Following Simultaneous Oral Administration of Intrinsic Factor-Bound and Free Radioactive Vitamin B<sub>12</sub>

Finney¹,

Payne²

1972

Acta Haematol

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“…This may also account for the clear separation of results obtained from patients with pernicious anaemia when the dose was given alone and with intrinsic factor. In addition to this clear separation of results in the region of 1618% dose retained, the ratio of amount retained when the dose was given with intrinsic factor to amount retained when the dose was given alone was always greater than 2, a point which is of diagnostic value (Katz, DiMase & Donaldson, 1963;Bell, Bridges & Nelson, 1965;Bell & Lee, 1969;Boddy, Will & Holmes, 1969). The tests on patients with pernicious anaemia were done separately and the procedure took 4 weeks.…”

Section: Absorption Of Cobalamin At High Dose Levelsmentioning

confidence: 99%

“…The tests on patients with pernicious anaemia were done separately and the procedure took 4 weeks. A further asset of the double-tracer technique is that it can be used to halve the time-scale by combination of the two tests into a single procedure (Donaldson & Katz, 1963 ;Bell et al, 1965;Bell & Lee, 1969;Boddy el al., 1969).…”

Section: Absorption Of Cobalamin At High Dose Levelsmentioning

confidence: 99%

Factors affecting the Absorption of Vitamin B12

et al. 1972

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1. The absorption of radioactive cobalamin was measured by a whole-body counting technique in control subjects and in patients with pernicious anaemia.2. The absorption of cyanocobalamin by patients with pernicious anaemia was decreased by charcoal but not by bile or saliva.3. The absorption of cyanocobalamin by control subjects was not affected by food but was significantly increased by pentagastrin. With pentagastrin the absorption of cyanocobalamin was significantly greater than that of hydroxocobalamin. The hog intrinsic factor-mediated absorption of cyanocobalamin by patients with pernicious anaemia was significantly depressed by pentagastrin.4. The effect of increasing the mass of hog intrinsic factor concentrate on the absorption of cyanocobalamin by patients with pernicious anaemia could be described by a function relating the amount absorbed, the mass of intrinsic factor and two constants. The relationship implies that when the mass of intrinsic factor is small the amount of cyanocobalamin absorbed is directly proportional to the mass of intrinsic factor but that absorption approaches a saturation value with increasing mass of intrinsic factor. In physiological terms the function implies that absorption is proportional to the amount of cyanocobalamin attached to receptor sites but that cyanocobalamin attached to receptor sites may become detached and either reattached or lost to absorption. 5. With oral doses of 25 pg and 50 pg, control subjects absorbed more cyanocobalamin and hydroxocobalamin than patients with pernicious anaemia. At both dose levels control subjects absorbed more cyanocobalamin than hydroxocobalamin but no difference was observed in patients with pernicious anaemia. The intrinsic factor mechanism therefore influences amounts absorbed at such dose levels and appears to be a factor in the differences in absorption of cyanocobalamin and hydroxocobalamin.6. The use of double-tracer techniques makes it possible for each subject to act as

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The gastrointestinal tract

Croft¹,

Williams²

1991

Clinical Nuclear Medicine

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Evaluation of a Dual Radioisotope Urinary Excretion Test in the Diagnosis of Pernicious Anaemia

Cited by 10 publications

References 3 publications

Plasma Radioactivity Following Simultaneous Oral Administration of Intrinsic Factor-Bound and Free Radioactive Vitamin B<sub>12</sub>

Plasma Radioactivity Following Simultaneous Oral Administration of Intrinsic Factor-Bound and Free Radioactive Vitamin B<sub>12</sub>

Factors affecting the Absorption of Vitamin B12

The gastrointestinal tract

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