Evaluation of a DNA Microarray (Check-MDR CT102) for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 Carbapenemases

Naas, Thierry; Cuzon, Gaëlle; Bogaerts, Pierre; Glupczynski, Youri; Nordmann, Patrice

doi:10.1128/jcm.02607-10

Cited by 125 publications

(96 citation statements)

References 34 publications

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“…Several in-house multiplex PCRs and commercial real-time PCR and DNA microarray methods performed well for the detection of different types of carbapenemases in Enterobacteriaceae (8,9). The in-house multiplex PCR from our study included primers for the detection of bla KPC , bla NDM , bla OXA-48-like , bla VIM , and bla IMP and had a sensitivity and specificity of 100% ( Table 2).…”

Section: Resultsmentioning

confidence: 93%

Laboratory Detection of Enterobacteriaceae That Produce Carbapenemases

Doyle

Peirano

Lascols³

et al. 2012

J Clin Microbiol

293

175

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A study was designed to evaluate the modified Hodge test (MHT), Mastdiscs ID inhibitor combination disks (MDI), Rosco Diagnostica Neo-Sensitabs (RDS), metallo-␤-lactamase (MBL) Etest, and in-house multiplex PCR for the detection of well-characterized carbapenemase-producing Enterobacteriaceae. One hundred forty-two nonrepeat clinical isolates of carbapenemase-producing Enterobacteriaceae (including Klebsiella spp., Escherichia coli, Citrobacter freundii, and Enterobacter spp.) obtained from the SMART worldwide surveillance program during 2008 to 2009 were included. These included 49 KPC-, 27 NDM-, 19 VIM-, 14 OXA-48-like enzyme-, and 5 IMP-producing isolates and 28 carbapenem-resistant, carbapenemase-negative isolates. The manufacturer's instructions were followed for MDI, RDS, and MBL Etest and CLSI guidelines for MHT. A multiplex PCR was designed to detect KPC, NDM, VIM, IMP, and OXA-48-like carbapenemases. Overall, the sensitivity and specificity were 78% and 93% for MDI, 80% and 93% for RDS, 58% and 93% for MHT, and 55% and 100% for MBL Etest, respectively. The PCR had 100% sensitivity and specificity. MDI and RDS performed well for the detection of KPCs and NDMs but poorly for VIMs, IMPs, and OXA-48-like enzymes. MHT performed well for KPCs and OXA-48-like enzymes but poorly for NDMs, VIMs, and IMPs. MDI and RDS were easy to perform and interpret but lacked sensitivity for OXA-48-like enzymes, VIMs, and IMPs. MHT and MBL Etest were often difficult to interpret. We recommend using molecular tests for the optimal detection of carbapenemase-producing Enterobacteriaceae.

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Section: Resultsmentioning

confidence: 93%

Laboratory Detection of Enterobacteriaceae That Produce Carbapenemases

Doyle

Peirano

Lascols³

et al. 2012

J Clin Microbiol

293

175

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“…Whole-cell DNAs were extracted from overnight bacterial cultures using the Mo Bio UltraClean™ Microbial DNA Isolation Kit. Microarray assays were performed according to the manufacturer's instructions with provided tubes and reagents as previously described [12][13].…”

Section: Genotypic Testing For Esbl and Carbapenemases Genesmentioning

confidence: 99%

Comparison of Molecular and Phenotypic Methods for the Detection and Characterization of Carbapenem Resistant Enterobacteriaceae

Somily

Garaween

Abukhalid

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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In recent years, there has been a rapid dissemination of carbapenem resistant Enterobacteriaceae (CRE). This study aimed to compare phenotypic and molecular methods for detection and characterization of CRE isolates at a large tertiary care hospital in Saudi Arabia. This study was carried out between January 2011 and November 2013 at the King Khalid University Hospital (KKUH) in Saudi Arabia. Determination of presence of extended-spectrum beta-lactamases (ESBL) and carbapenem resistance was in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. Phenotypic classification was done by the MASTDISCSTM ID inhibitor combination disk method. Genotypic characterization of ESBL and carbapenemase genes was performed by the Check-MDR CT102. Diversilab rep-PCR was used for the determination of clonal relationship. Of the 883 ESBL-positive Enterobacteriaceae detected during the study period, 14 (1.6%) isolates were carbapenem resistant. Both the molecular genotypic characterization and phenotypic testing were in agreement in the detection of all 8 metalo-beta-lactamases (MBL) producing isolates. Of these 8 MBL-producers, 5 were positive for blaNDM gene and 3 were positive for blaVIM gene. Molecular method identified additional blaOXA gene isolates while MASTDISCSTM ID detected one AmpC producer isolate. Both methods agreed in identifying 2 carbapenem resistant isolates which were negative for carbapenemase genes. Diversilab rep-PCR analysis of the 9 Klebsiella pneumoniae isolates revealed polyclonal distribution into eight clusters. MASTDISCSTM ID is a reliable simple cheap phenotypic method for detection of majority of carbapenemase genes with the exception of the blaOXA gene. We recommend to use such method in the clinical laboratory.

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“…The Check-KPC ESBL assay has a detection profile similar to that of the Check-ESBL and additionally detects bla KPC . The assay evaluation showed excellent ESBL and bla KPC detection sensitivities and specificities ranging from 95% to 100% and 99% to 100%, respectively (see Table S1) (7,12,20). Failure to detect ESBLs was primarily because some gene probes were not included on the array (e.g., bla PER and bla GES ), although a few bla TEM and bla SHV ESBLs were also missed (7,12).…”

Section: Molecular Detection Of Esbl-harboring and Carbapenem-resistamentioning

confidence: 99%

Current Trends in Culture-Based and Molecular Detection of Extended-Spectrum-β-Lactamase-Harboring and Carbapenem-Resistant Enterobacteriaceae

et al. 2012

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The ever-expanding role of extended-spectrum-␤-lactamase (ESBL)-harboring and carbapenem-resistant Enterobacteriaceae in causing serious infections poses a grave public health threat because these organisms also tend to be multidrug resistant, for which very few (if any) antibiotic options remain available. Rapid detection of such panresistant organisms offers one of the best solutions to improve patient screening and hospital infection control practices as well as curb inappropriate antibiotic use. This review discusses and compares primarily the current state-of-the-art culture-based and molecular methods that are commercially available for detection or screening of ESBL-harboring and carbapenem-resistant Enterobacteriaceae.

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Evaluation of a DNA Microarray (Check-MDR CT102) for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 Carbapenemases

Cited by 125 publications

References 34 publications

Laboratory Detection of Enterobacteriaceae That Produce Carbapenemases

Laboratory Detection of Enterobacteriaceae That Produce Carbapenemases

Comparison of Molecular and Phenotypic Methods for the Detection and Characterization of Carbapenem Resistant Enterobacteriaceae

Current Trends in Culture-Based and Molecular Detection of Extended-Spectrum-β-Lactamase-Harboring and Carbapenem-Resistant Enterobacteriaceae

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