Evaluation of a digital dispenser for direct curve dilutions in a vaccine potency assay

Roselle, Christopher; Whitehouse, Dana; Follmer, Thy; Ansbro, Fran; Bouaraphan, Silikhone; Guan, Liming; Wang, Sha-Ke; Shank-Retzlaff, Mary; Verch, Thorsten

doi:10.1016/j.jim.2016.12.003

Journal of Immunological Methods

2017

DOI: 10.1016/j.jim.2016.12.003

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Evaluation of a digital dispenser for direct curve dilutions in a vaccine potency assay

Christopher Roselle

Dana Whitehouse

Thy Follmer

et al.

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Cited by 5 publications

(1 citation statement)

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“…Inkjet dispensing technologies have been harnessed for biomedical applications − where they are utilized for the precise placement of droplets with controlled volumes. We take advantage of this attribute to develop a workflow for the calibration of SERS substrates, which we call Inkjet Dispense SERS (ID-SERS).…”

mentioning

confidence: 99%

A Generalizable Single-Chip Calibration Method for Highly Quantitative SERS via Inkjet Dispense

D’Apuzzo¹,

Sengupta²,

Overbay³

et al. 2019

Anal. Chem.

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We present a calibration method for quantitative surface-enhanced Raman scattering (SERS) on a single-chip based on inkjet dispense (ID-SERS). We exploit the ability of inkjet to precisely pattern microdroplets at high resolution to encode multiple standard curves on the surface of a single 1 mm2 SERS substrate. We demonstrate quantitative SERS measurements with a relative standard error (RSE) below 3% for aqueous solutions of 1,2-bis(4-pyridyl)ethylene (BPE), the lowest reported to date. Most importantly, the RSE scales with patterning density and sensor size, showing the potential for even higher measurement accuracy. This calibration technique can be generalized to other plasmonic substrates and offers several additional advantages including speed (subsecond drop-and-dry), low sample volumes (<1 nL), and automation. Finally, we investigate factors impacting the limit of detection of this approach and demonstrate a 30-fold enhancement of sensitivity via layered inkjet dispense. We believe that ID-SERS paves the way for the development of reproducible plasmonic sensing for real-world quantitative applications.

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mentioning

confidence: 99%

A Generalizable Single-Chip Calibration Method for Highly Quantitative SERS via Inkjet Dispense

D’Apuzzo¹,

Sengupta²,

Overbay³

et al. 2019

Anal. Chem.

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Quantitation of Super Basic Peptides in Biological Matrices by a Generic Perfluoropentanoic Acid-Based Liquid Chromatography–Mass Spectrometry Method

Wen

Wang

Lee

et al. 2019

J. Am. Soc. Mass Spectrom.

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Use of capillary-mediated vitrification to produce thermostable, single-use antibody conjugates as immunoassay reagents

Amle¹,

Radford²,

Wang³

et al. 2023

Journal of Immunological Methods

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Evaluation of a digital dispenser for direct curve dilutions in a vaccine potency assay

Cited by 5 publications

References 26 publications

A Generalizable Single-Chip Calibration Method for Highly Quantitative SERS via Inkjet Dispense

A Generalizable Single-Chip Calibration Method for Highly Quantitative SERS via Inkjet Dispense

Quantitation of Super Basic Peptides in Biological Matrices by a Generic Perfluoropentanoic Acid-Based Liquid Chromatography–Mass Spectrometry Method

Use of capillary-mediated vitrification to produce thermostable, single-use antibody conjugates as immunoassay reagents

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