Evaluation of a Commercial Real-Time Reverse Transcription Polymerase Chain Reaction Kit for the Diagnosis of <i>Bovine Respiratory Syncytial Virus</i> Infection

Timsit, Edouard; Maingourd, Cyril; Dréan, Eric Le; Belloc, Catherine; Seegers, Henri H.; Douart, Alain; Assié, Sébastien

doi:10.1177/104063871002200211

Cited by 15 publications

(14 citation statements)

References 19 publications

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“…A commercial kit detects all bRSV strains assessed with 99.3% efficiency and a detection limit of 0.1 TCID 50 (50% tissue culture infective dose). 83 This kit had much higher sensitivity than that of fluorescent antibody assays. A 1-step multiplex real-time RT-PCR assay for bRSV, bovine herpesvirus 1, and bovine parainfluenza virus 3 was also found to be more sensitive than immunofluorescence (and virus isolation).…”

Section: Strain Variabilitymentioning

confidence: 99%

Respiratory Syncytial Virus Infection in Cattle

et al. 2013

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Bovine respiratory syncytial virus (RSV) is a cause of respiratory disease in cattle worldwide. It has an integral role in enzootic pneumonia in young dairy calves and summer pneumonia in nursing beef calves. Furthermore, bovine RSV infection can predispose calves to secondary bacterial infection by organisms such as Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni, resulting in bovine respiratory disease complex, the most prevalent cause of morbidity and mortality among feedlot cattle. Even in cases where animals do not succumb to bovine respiratory disease complex, there can be long-term losses in production performance. This includes reductions in feed efficiency and rate of gain in the feedlot, as well as reproductive performance, milk production, and longevity in the breeding herd. As a result, economic costs to the cattle industry from bovine respiratory disease have been estimated to approach $1 billion annually due to death losses, reduced performance, and costs of vaccinations and treatment modalities. Human and bovine RSV are closely related viruses with similarities in histopathologic lesions and mechanisms of immune modulation induced following infection. Therefore, where appropriate, we provide comparisons between RSV infections in humans and cattle. This review article discusses key aspects of RSV infection of cattle, including epidemiology and strain variability, clinical signs and diagnosis, experimental infection, gross and microscopic lesions, innate and adaptive immune responses, and vaccination strategies.

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Section: Strain Variabilitymentioning

confidence: 99%

Respiratory Syncytial Virus Infection in Cattle

et al. 2013

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“…Various techniques including cell culture, serology (fluorescent antibody tests, ELISA) and nucleic acid testing are used in laboratory diagnosis of viral infections in BRD (Albayrak et al, 2019;Timsit et al, 2010). These differ in sensitivity and specificity, with none an optimal reference standard able to detect all viruses in all clinical situations.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of novel multiplex qPCR assays for diagnosis of pathogens associated with the bovine respiratory disease complex

Pansri

Katholm

Krogh

et al. 2020

The Veterinary Journal

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A B S T R A C TBovine respiratory disease complex is the most common disease requiring the use of antimicrobials in industrial calf production worldwide. Pathogenic bacteria (Mannheimia haemolytica (Mh), Pasteurella multocida (Pm), Histophilus somni (Hs), and Mycoplasma bovis) and a range of viruses (bovine respiratory syncytial virus, bovine coronavirus, bovine parainfluenza virus type 3, bovine viral diarrhea virus and bovine herpesvirus type 1) are associated with this complex. As most of these pathogens can be present in healthy and diseased calves, simple detection of their presence in diseased calves carries low predictive value. In other multi-agent diseases of livestock, quantification of pathogens has added substantially to the predictive value of microbiological diagnosis. The aim of this study was to evaluate the ability of two recently developed quantitative PCR (qPCR) kits (Pneumo4B and Pneumo4V) to detect and quantify these bacterial and viral pathogens, respectively.Test efficiencies of the qPCR assays, based on nucleic acid dilution series of target bacteria and viruses, were 93-106% and 91-104%, respectively, with assay detection limits of 10-50 copies of nucleic acids. All 44 strains of target bacteria were correctly identified, with no false positive reactions in 135 strains of non-target bacterial species. Based on standard curves of log 10 CFU versus cycle threshold (Ct) values, quantification was possible over a 5-log range of bacteria. In 92 tracheal aspirate samples, the kappa values for agreement between Pneumo4B and bacterial culture were 0.64-0.84 for Mh, Pm and Hs. In an additional 84 tracheal aspirates, agreement between Pneumo4B or Pneumo 4V and certified diagnostic qPCR assays was moderate (0.57) for M. bovis and high (0.71-0.90) for viral pathogens. Thus Pneumo4 kits specifically detected and quantified the relevant pathogens.

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“…Prior to the widespread application of PCR methods in veterinary diagnostic laboratories, commonly used methods for the detection of BRSV and BPIV3 were fluorescent antibody testing (FAT) on frozen tissue sections [ 104 – 106 ], lung lavage samples [ 107 ] or nasal swab samples [ 108 , 109 ] and Enzyme-Linked Immunosorbent Assay (ELISA) testing of organ homogenates [ 110 ]. In general, PCR testing has a better sensitivity than the traditional methods like virus isolation, ELISA and FAT [ 111 , 112 ]. Currently, different multiplex formats that allow testing of BRSV and BPIV3 together with other agents within the BRD complex have been developed.…”

Section: Main Textmentioning

confidence: 99%

Review on bovine respiratory syncytial virus and bovine parainfluenza – usual suspects in bovine respiratory disease – a narrative review

Makoschey¹,

Berge

2021

BMC Vet Res

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Bovine Respiratory Syncytial virus (BRSV) and Bovine Parainfluenza 3 virus (BPIV3) are closely related viruses involved in and both important pathogens within bovine respiratory disease (BRD), a major cause of morbidity with economic losses in cattle populations around the world. The two viruses share characteristics such as morphology and replication strategy with each other and with their counterparts in humans, HRSV and HPIV3. Therefore, BRSV and BPIV3 infections in cattle are considered useful animal models for HRSV and HPIV3 infections in humans.The interaction between the viruses and the different branches of the host’s immune system is rather complex. Neutralizing antibodies seem to be a correlate of protection against severe disease, and cell-mediated immunity is thought to be essential for virus clearance following acute infection. On the other hand, the host’s immune response considerably contributes to the tissue damage in the upper respiratory tract.BRSV and BPIV3 also have similar pathobiological and epidemiological features. Therefore, combination vaccines against both viruses are very common and a variety of traditional live attenuated and inactivated BRSV and BPIV3 vaccines are commercially available.

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Evaluation of a Commercial Real-Time Reverse Transcription Polymerase Chain Reaction Kit for the Diagnosis of Bovine Respiratory Syncytial Virus Infection

Cited by 15 publications

References 19 publications

Respiratory Syncytial Virus Infection in Cattle

Respiratory Syncytial Virus Infection in Cattle

Evaluation of novel multiplex qPCR assays for diagnosis of pathogens associated with the bovine respiratory disease complex

Review on bovine respiratory syncytial virus and bovine parainfluenza – usual suspects in bovine respiratory disease – a narrative review

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