Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections

Josefson, P.; Strålin, Kristoffer; Ohlin, Andreas; Ennefors, Theresa; Dragsten, B.; Andersson, Lena; Fredlund, Hans; Mölling, Paula; Olcén, Per

doi:10.1007/s10096-011-1201-6

Cited by 63 publications

(47 citation statements)

References 22 publications

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“…These results stand in contrast to the results of our study, where the blood cultures showed a higher pathogen detection rate. Other recent research is in line with our results: studies investigating patients with suspected sepsis in the emergency department [21], patients with malignancies and sepsis [22], critically ill neutropenia patients [23] and etiological diag- nosis of community-onset bloodstream infections [24]. The other two PCR assays (SepsiTest® and VYOO®) are less well studied in respect to sepsis.…”

Section: Discussionsupporting

confidence: 88%

Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients

Schreiber

Nierhaus

Braune

et al. 2013

Med Klin Intensivmed Notfmed

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Section: Discussionsupporting

confidence: 88%

Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients

Schreiber

Nierhaus

Braune

et al. 2013

Med Klin Intensivmed Notfmed

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“…In some of these studies, the SeptiFast assay was found to be clearly superior, 9,11,21,22 whereas in other studies, both methods displayed either similar sensitivities or the SeptiFast assay compared unfavorably with respect to blood culture. 12,[23][24][25] Differences between studies in terms of patients' characteristics, the spectrum of microorganisms detected, the number of blood culture sets collected, the volume of blood collected for culture, and the presence or absence of ongoing antibiotic therapy at the time of sampling may account for these discrepancies. Inappropriate antimicrobial treatment or delays in starting appropriate treatment are both associated with increased morbidity and mortality in sepsis, particularly in severe cases.…”

Section: Discussionmentioning

confidence: 99%

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

Suberviola

Márquez-López

Castellanos-Ortega

et al. 2015

American Journal of Critical Care

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Objective To compare the utility of a multiplex polymerase chain reaction system (SeptiFast) and blood cultures for detecting bacteria and fungi in blood samples from patients with severe sepsis or septic shock. Methods In a prospective observational study, whole blood samples for SeptiFast testing and for culture were collected on admission from all patients with severe sepsis or septic shock admitted to the intensive care unit between July 2011 and September 2012. SeptiFast results were compared with blood and other culture results. Results The probability of at least 1 microorganism being isolated at 6 hours was 13-fold higher with the SeptiFast test than with blood cultures (relative risk, 13.5; 95% CI, 5.05-36.06). Unlike culture results, SeptiFast test results were not associated with previous antibiotic consumption. The median time to the first positive blood culture result was 17 hours; SeptiFast results were available in 6 hours. SeptiFast detected genetic material from potentially multiresistant microorganisms in patients whose blood cultures showed no growth at all. Conclusions The SeptiFast test provided quicker microbiological diagnosis and identified significantly more microorganisms than blood cultures did, particularly when samples were collected after antibiotic therapy had started or infections were due to resistant bacteria and yeast.

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“…SeptiFast (SF; Roche Diagnostics GmbH, Mannheim, Germany), a commercially available PCR-based system, has been used in the molecular diagnosis of sepsis in patients from emergency room (14) and intensive care units (12,15,18), in neutropenic and immunocompromised febrile patients (9,16,20), as well as in patients with endocarditis (4). Data regarding the diagnostic usefulness of SF in the routine clinical management of patients with suspected sepsis hospitalized in the tertiary care units are still limited (5,8,21).…”

mentioning

confidence: 99%

Diagnostic Performance of a Multiple Real-Time PCR Assay in Patients with Suspected Sepsis Hospitalized in an Internal Medicine Ward

et al. 2012

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Early identification of causative pathogen in sepsis patients is pivotal to improve clinical outcome. SeptiFast (SF), a commercially available system for molecular diagnosis of sepsis based on PCR, has been mostly used in patients hospitalized in hematology and intensive care units. We evaluated the diagnostic accuracy and clinical usefulness of SF, compared to blood culture (BC), in 391 patients with suspected sepsis, hospitalized in a department of internal medicine. A causative pathogen was identified in 85 patients (22%). Sixty pathogens were detected by SF and 57 by BC. No significant differences were found between the two methods in the rates of pathogen detection (P ‫؍‬ 0.74), even after excluding 9 pathogens which were isolated by BC and were not included in the SF master list (P ‫؍‬ 0.096). The combination of SF and BC significantly improved the diagnostic yield in comparison to BC alone (P < 0.001). Compared to BC, SF showed a significantly lower contamination rate (0 versus 19 cases; P < 0.001) with a higher specificity for pathogen identification (1.00, 95% confidence interval [CI] of 0.99 to 1.00, versus 0.94, 95% CI of 0.90 to 0.96; P ‫؍‬ 0.005) and a higher positive predictive value (1.00, 95% CI of 1.00 to 0.92%, versus 0.75, 95% CI of 0.63 to 0.83; P ‫؍‬ 0.005). In the subgroup of patients (n ‫؍‬ 191) who had been receiving antibiotic treatment for >24 h, SF identified more pathogens (16 versus 6; P ‫؍‬ 0.049) compared to BC. These results suggest that, in patients with suspected sepsis, hospitalized in an internal medicine ward, SF could be a highly valuable adjunct to conventional BC, particularly in patients under antibiotic treatment.

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Evaluation of a commercial multiplex PCR test (SeptiFast) in the etiological diagnosis of community-onset bloodstream infections

Cited by 63 publications

References 22 publications

Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients

Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

Diagnostic Performance of a Multiple Real-Time PCR Assay in Patients with Suspected Sepsis Hospitalized in an Internal Medicine Ward

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