Evaluation of a commercial enzyme immunoassay versus culture for the detection ofChlamydia trachomatis

Bäckman, M.; Rudén, A K; Ringertz, Olof; Sandström, Erik

doi:10.1007/bf02185844

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…This koala had antichlamydial antibodies but no clinical signs of disease at the time of sampling or during the previous year. Koalas 1,2, and 3 were all diagnosed as having chlamydial infections in the previous 6 months, and both koalas 1 and 2 had clinical signs of dirty tail at the time of sampling.…”

Section: Resultsmentioning

confidence: 99%

“…Results from the ELISAs, which were read with a spectrophotometer (IDEIA, Pathfinder, Chlamydiazyme, and Chlamydia-EIA), were sometimes difficult to interpret because sample absorbances were often close to the cutoff zone recommended by the manufacturer. The need for an equivocal zone around the cutoff value has been stressed by other workers (1,14).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas

Wood

1992

J Clin Microbiol

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Chlamydia psiktaci is the major cause of infectious disease in the koala (Phascolarctos cinereus). It causes four disease syndromes in the koala, namely, conjunctivitis, rhinitis, cystitis, and infertility (females only). Diagnosis of chlamydial infections in koalas relies primarily on isolation of the organism in cell culture. Serology has generally not been useful, and little use has previously been made of the commercially available antigen detection kits. We examined the sensitivity, specificity, and usefulness of three direct fluorescentantibody kits (Vet-IF [Cell Labs], IMAGEN [Celltechl, Chlamydia-Direct IF [Bio Merieux]) and six antigen detection enzyme-linked immunosorbent assay (ELISA) kits (Clearview [Unipathl, Surecell [Kodak], Pathfinder [Kallestad], Chlamydia-EIA [Pharmacia], Chlamydiazyme [Abbott], IDEIA [Celltech]) for the detection of urogenital infections in koalas. Laboratory studies showed that the direct fluorescent-antibody kits were the least sensitive in this case and did not detect fewer than 104 elementary bodies per ml, while most ELISA kits detected between 130 and 600 elementary bodies per ml. Field study results showed that the Clearview kit was the most sensitive (91%) compared with the IDEIA (88%) and the Surecell (73%) kits. All three kits were more sensitive than cell culture (36%), highlighting viability loss problems that occur during transport. This study showed that the Clearview kit is sensitive, specific, and easy to use for the detection of type II (urogenital) C. psittaci from koalas in the field and warrants further evaluation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas

Wood

1992

J Clin Microbiol

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“…The use of an equivocal zone around the cut-off absorbance value has been discussed with respect to genital samples (4). This might also be applied in the case of urine specimens.…”

Section: Materials and Methods A Total Of 167 Menmentioning

confidence: 99%

Detection ofChlamydia trachomatis in urine from men with urethritis

Bäckman¹,

Rudén

Ringertz³

et al. 1993

Eur. J. Clin. Microbiol. Infect. Dis.

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The performance of a commercial EIA (Chlamydiazyme) for detection of Chlamydia trachomatis in urine specimens was compared with that of culture of urethral samples from men with urethritis. The incidence of chlamydial infection on the basis of culture results was 34% (56/167). The sensitivity, specificity, positive and negative predictive values for the EIA were 55% (31/56), 98% (109/111), 94% (31/33) and 81% (109/134), respectively, compared with culture. Although this EIA has a high specificity, the low sensitivity makes it valueless as a clinical tool for demonstrating chlamydial antigen in urine from men with urethritis.

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“…The former two methods, although very time-consuming, are known to be more sensitive than the isolation of the organism in cell culture (5,19). Antigen-detection systems utilizing enzymelinked immunoassays or immunofluorescent antibody (IFA) techniques have been extensively introduced to detect chlamydial infections (1,6,8,21,23). New methods which detect chlamydial nucleic acid in specimens, such as DNA hybridization and polymerase chain reaction (PCR), have recently been used for the detection of chlamydiae (2,13,14).…”

mentioning

confidence: 99%

Polymerase Chain Reaction for the Detection of Chlamydia psittaci in the Feces of Budgerigars

Takashima

Imai

Itoh

et al. 1996

Microbiology and Immunology

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The polymerase chain reaction (PCR) targeting the ompA gene of Chlamydia psittaci was evaluated for its ability to detect chlamydiae in fecal specimens of budgerigars as compared with isolation procedures using cell culture and embryonated egg inoculations. Several procedures for PCR template DNA preparation were compared so as to determine their detection levels for chlamydiae propagated in cell culture in the presence of fecal materials. Tween-20 and proteinase K treatments followed by centrifugation of the template DNA were found to be an appropriate procedure for DNA preparation for primary PCR. Subsequent nested PCR was shown to detect 4.8 IFU/ml or 84 particles/ml of chlamydiae. Chlamydiae in 50 fecal specimens from apparently healthy budgerigars were examined by nested PCR and several other methods. Nested PCR detected chlamydiae at a higher rate (12/50, 24%) than the isolation procedure in embryonated eggs (6/50, 12%). Primary PCR combined with the isolation procedure in cell culture gave a detection rate (5/50, 10%) similar to that of isolation from embryonated eggs. Detection rates by primary PCR (1/50, 2%) and in cell culture (0%) were inferior to the other procedures.

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Evaluation of a commercial enzyme immunoassay versus culture for the detection ofChlamydia trachomatis

Cited by 8 publications

References 11 publications

Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas

Comparison of nine antigen detection kits for diagnosis of urogenital infections due to Chlamydia psittaci in koalas

Detection ofChlamydia trachomatis in urine from men with urethritis

Polymerase Chain Reaction for the Detection of Chlamydia psittaci in the Feces of Budgerigars

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