Evaluation of a Commercial Colloidal Gold Assay for Detection of Influenza A and B Virus in Children’s Respiratory Specimens

Li, Wei; Liu, Lifang; Chen, Luyan; Shang, Shiqiang

doi:10.1080/15513815.2019.1639088

Cited by 10 publications

(6 citation statements)

References 17 publications

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“…Accessed 15 March 2020). The incorporation of colloidal gold-labeled immunoglobulin G (IgG) as the detection reagent is an approach that may increase the sensitivity of rapid antigen tests for respiratory viruses [38]. Monoclonal antibodies specifically against SARS-CoV-2 have been under preparation.…”

Section: Rapid Antigen Testsmentioning

confidence: 99%

Laboratory diagnosis of emerging human coronavirus infections – the state of the art

Loeffelholz¹,

Tang

2020

Emerging Microbes & Infections

703

708

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The three unprecedented outbreaks of emerging human coronavirus (HCoV) infections at the beginning of the twentyfirst century have highlighted the necessity for readily available, accurate and fast diagnostic testing methods. The laboratory diagnostic methods for human coronavirus infections have evolved substantially, with the development of novel assays as well as the availability of updated tests for emerging ones. Newer laboratory methods are fast, highly sensitive and specific, and are gradually replacing the conventional gold standards. This presentation reviews the current laboratory methods available for testing coronaviruses by focusing on the coronavirus disease 2019 (COVID-19) outbreak going on in Wuhan. Viral pneumonias typically do not result in the production of purulent sputum. Thus, a nasopharyngeal swab is usually the collection method used to obtain a specimen for testing. Nasopharyngeal specimens may miss some infections; a deeper specimen may need to be obtained by bronchoscopy. Alternatively, repeated testing can be used because over time, the likelihood of the SARS-CoV-2 being present in the nasopharynx increases. Several integrated, random-access, point-of-care molecular devices are currently under development for fast and accurate diagnosis of SARS-CoV-2 infections. These assays are simple, fast and safe and can be used in the local hospitals and clinics bearing the burden of identifying and treating patients.

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Section: Rapid Antigen Testsmentioning

confidence: 99%

Laboratory diagnosis of emerging human coronavirus infections – the state of the art

Loeffelholz¹,

Tang

2020

Emerging Microbes & Infections

703

708

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“…From January through March 2018, nasal swab specimens from 9914 patients with influenza-like symptoms were collected and tested for influenza viral antigens (Li et al, 2019) using the Clearview R Exact Influenza A&B kit with the colloidal gold detection method (Jennings et al, 2009) 2 . Briefly, each nasal swab was eluted with 192 µL lysis buffer and the immunochromatographic strip in the kit was inserted into the eluent and incubated for 15 min at room temperature.…”

Section: Influenza Diagnostic Testsmentioning

confidence: 99%

Characterization of Influenza A and B Viruses Circulating in Southern China During the 2017–2018 Season

Yan

Zhao

et al. 2020

Front. Microbiol.

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“…Of note, there was no influenza A or B detected during study period. In winter of 2018 to 2019, we used a commercial colloidal gold assay to detect RSV, HADV, IAV, and IBV, 10 11 The positive rates of RSV, HADV, IAV, and IBV were 9.15% (8,933/97,657), 9.65% (9,419/97,656), 21.5% (19,494/92,191), and 2.58% (2,376/92,216). There is a significant difference in the distribution of RSV, HADV, IAV, and IBV between winter of 2020 to 2021 and 2018 to 2019 ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

The Impact of COVID-19 Public Health Measures on Detection of Other Respiratory Viruses in Children during the Winter of 2020–2021 in Hangzhou, China

Xiang

Lin

Guo

et al. 2022

J Pediatr Infect Dis

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Objective Acute respiratory tract infection (ARTI) is one of the main diseases in childhood. This study aimed to monitor the distribution of respiratory tract viruses in children with ARTI in the impact of coronavirus disease 2019 (COVID-19). Methods We conducted surveillance of 2019 novel coronavirus, human metapneumovirus, respiratory syncytial virus, human adenovirus, human parainfluenza virus 1–3, and influenza A and B virus by using quantitative real-time polymerase chain reaction. Results During the winter of 2020 to 2021, among the 1,442 throat swabs we collected, 937 (64.98%, 937/1,442) were positive for respiratory viruses. Respiratory syncytial virus was the most frequently detected respiratory virus (34.12%, 492/1,442) and 2019 novel coronavirus and influenza A and B virus were not detected in the study period. Coinfection was observed in 156 positive samples including 149 samples of double infection and 7 of triple infection. The positive rate of viral respiratory tract infection in infants less than 6 months was the highest (72.95%) in the study period. Conclusion There are some differences in the distribution of respiratory viruses in children after the outbreak of COVID-19 in Hangzhou, China.

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Evaluation of a Commercial Colloidal Gold Assay for Detection of Influenza A and B Virus in Children’s Respiratory Specimens

Cited by 10 publications

References 17 publications

Laboratory diagnosis of emerging human coronavirus infections – the state of the art

Laboratory diagnosis of emerging human coronavirus infections – the state of the art

Characterization of Influenza A and B Viruses Circulating in Southern China During the 2017–2018 Season

The Impact of COVID-19 Public Health Measures on Detection of Other Respiratory Viruses in Children during the Winter of 2020–2021 in Hangzhou, China

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