Evaluation of a Commercial Automated System and Software for the Identification of Veterinary Bacterial Isolates

Abstract

Abstract. A commercial gram-negative bacterial autoidentification plate was originally developed using bacterial isolates of human origin. Three veterinary diagnostic laboratories conducted a 2-phase trial to enhance the database for veterinary use. The first phase consisted of testing the plate with 447 bacterial isolates of veterinary origin and incorporating that data into the existing database. Emphasis was placed on the Actinobacillus, Bordetella, Pasteurella and Enterobacteriaceae groups, since the Pseudomonas taxon was quite complete. The second phase of the trial consisted of evaluating the enhanced database using 270 clinical veterinary isolates normally encountered in veterinary laboratories. For the Actinobacillus, Bordetella, Pasteurella and Enterobacteriaceae groups, 72% of the bacterial isolates were identified correctly to genus and 61% to species after 5 hours incubation. Eighty-nine percent of the bacterial isolates were identified correctly to genus and 85% to species after 18 hours incubation. All identifications in phase 1 and phase 2 were confirmed using conventional methods.The bacteria frequently associated with infectious diseases of animals often fall into categories less commonly encountered in humans and, therefore, are not included in the databases of the automated identification systems used in human medicine. A 3-laboratory collaborative effort was made to enhance the database of an automated identification system for veterinary use. Subsequently, the 3 laboratories challenged the autoidentification 5-hour and 18-hour systems using fresh clinical isolates and compared the results with conventional methods. Material and methodsParticipant laboratories. This study was conducted by 3 laboratories representing different geographic regions and therefore different animal populations of the United States. They included the University of Georgia Veterinary Diagnostic Laboratory (Tifton, GA), the University of Missouri Veterinary Medical Diagnostic Laboratory (Columbia, MO), and the New York State Veterinary Diagnostic Laboratory at Cornell University (Ithaca, NY). Cornell Diagnostic Laboratory served as the coordinating laboratory. Each trial center used media and reagents consistent with their routine diagnostic testing. A protocol using conventional biochemical methods was defined by the coordinating laboratory to serve as the reference method for identifying test isolates. negative autoidentification system a supplied quality control (QC) organisms on freeze-dried gelatin discs maintained at 2-8 C. They included Escherichia coli ATCC 4157, Klebsiella oxytoca ATCC 8724, Morganella morganii ATCC 25830, Pseudomonas aeruginosa ATCC 10145, and Proteus vulgaris ATCC 6896. To prepare the organisms for testing, a disc was aseptically placed onto a blood agar plate (BAP) such as trypticase soy agar containing 5% sheep blood, incubated 5 min at 35-37 C to allow the disc to liquify, and then streaked across the entire plate. The plate was incubated for 18-24 hr at 35-37 C to determine purity and vi...

Microbiology of the external ear canal in six African elephants (Loxodonta africana)

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