Evaluation of a collagen-coated, resorbable fiber scaffold loaded with a peptide basic fibroblast growth factor mimetic in a sheep model of rotator cuff repair

Peterson, Dale R.; Ohashi, Kevin L.; Aberman, Harold M.; Piza, Pedro A.; Crockett, Heber C.; Fernandez, Joseph I.; Lund, Pamela J.; Funk, Kathleen A.; Hawes, Michael; Parks, Brent G.; Mattern, Ralph–Heiko

doi:10.1016/j.jse.2015.06.009

Cited by 25 publications

(17 citation statements)

References 25 publications

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“…For wound dressing, three-dimensional collagen sponges are often applied as they can absorb exudates and offer a natural barrier for bacterial infections [ 11 ]. Collagen is also applied as a coating material [ 17 , 18 ]. For example, Douglas and Haugen [ 19 ] described the coating of polyurethane scaffolds with collagen to improve the cell response.…”

Section: Introductionmentioning

confidence: 99%

Collagen as Coating Material for 45S5 Bioactive Glass-Based Scaffolds for Bone Tissue Engineering

Hum

Boccaccını

2018

IJMS

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Highly porous 45S5 bioactive glass-based scaffolds were fabricated by the foam replica technique and coated with collagen by a novel method. After an initial cleaning step of the bioactive glass surface to expose reactive –OH groups, samples were surface functionalized by (3-aminopropyl)triethoxysilane (APTS). Functionalized scaffolds were immersed in a collagen solution, left for gelling at 37 °C, and dried at room temperature. The collagen coating was further stabilized by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Applying this coating method, a layer thickness of a few micrometers was obtained without affecting the overall scaffold macroporosity. In addition, values of compressive strength were enhanced by a factor of five, increasing from 0.04 ± 0.02 MPa for uncoated scaffolds to 0.18 ± 0.03 MPa for crosslinked collagen-coated scaffolds. The composite material developed in this study exhibited positive cell (MG-63) viability as well as suitable cell attachment and proliferation on the surface. The combination of bioactivity, mechanical competence, and cellular response makes this novel scaffold system attractive for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Collagen as Coating Material for 45S5 Bioactive Glass-Based Scaffolds for Bone Tissue Engineering

Hum

Boccaccını

2018

IJMS

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“…However, the ovine model is not without limitations. 29,32,40 Most notably, the inability to limit weight bearing in a quadrupedal animal can lead to gap formation at the tendon-bone interface. Recently, human studies have shown similar gap formations to occur when repairs to the RTC are loaded acutely.…”

Section: Discussionmentioning

confidence: 99%

“…Movin' tendinopathy was a cumulative scoring of the tendon's fiber structure, fiber arrangement, rounding of nuclei, regional variation in cellularity, increased vascularity, and decreased collagen stainability, with each constituent being scored on a 3-point scale and then averaged across a single sample. 29 Fibroblasts were counted in 4Â to 40Â objective fields from H&E sections within 8 mm of the PLGA scaffold when present or within 10 mm of bone-tendon interface when the PLGA scaffold was absent.…”

Section: Histologic Analysismentioning

confidence: 99%

“…Various techniques have been employed to improve interface healing, including bone marrow venting; cellular therapies (mesenchymal stem cells); and xenograft, allograft, or acellular scaffolds. 27,29,32,36,41 Bone marrow vents have shown promise in improving the bone-tissue interface healing of many soft tissue injuries by allowing autologous marrow-derived cells to reach the repair site. 9,37 Bone marrow venting is a comparatively expeditious surgical procedure, has no issue of cellular incompatibility, and may be accomplished through vented tissue anchors or through separate bone puncture holes.…”

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confidence: 99%

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A prospective study comparing tendon-to-bone interface healing using an interposition bioresorbable scaffold with a vented anchor for primary rotator cuff repair in sheep

Easley

Puttlitz

Hackett

et al. 2020

Journal of Shoulder and Elbow Surgery

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Background: The purpose of this study was to evaluate the biomechanical and histologic properties of rotator cuff repairs using a vented anchor attached to a bioresorbable interpositional scaffold composed of aligned PLGA (poly(L-lactide-co-glycoside)) microfibers in an animal model compared to standard anchors in an ovine model. Methods: Fifty-six (n ¼ 56) skeletally mature sheep were randomly assigned to a repair of an acute infraspinatus tendon detachment using a innovative anchor-PLGA scaffold device (Treatment) or a similar anchor without the scaffold (Control). Animals were humanely euthanized at 7 and 12 weeks post repair. Histologic and biomechanical properties of the repairs were evaluated and compared. Results: The Treatment group had a significantly higher fibroblast count at 7 weeks compared to the Control group. The tendon bone repair distance, percentage perpendicular fibers, new bone formation at the tendon-bone interface, and collagen type III deposition was significantly greater for the Treatment

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“…In addition, basic fibroblast growth factor (FGF-2) has beneficial effects on healing including enhanced new bone formation and reduced delamination of the tendon-bone interface in sheep, but tendon thickness is increased, raising translational concerns for inducing sub-acromial impingement. 71 However, in rats FGF-2 delivery enhanced mechanical properties without increasing cross-sectional area, and increased collagen organization and expression of scleraxis and tenomodulin in the healing interface. 72 Direct delivery of platelet-derived growth factor-BB (PDGF-BB) improved collagen organization, and mechanical properties but not regeneration of fibrocartilage at the rotator cuff tendon-bone interface in rats.…”

Section: Cells and Differentiation Pathwaysmentioning

confidence: 98%

Current Status of Tissue-engineered Scaffolds for Rotator Cuff Repair

Chainani

Little

2016

Techniques in Orthopaedics

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Rotator cuff tears continue to be at significant risk for re-tear or for failure to heal after surgical repair despite the use of a variety of surgical techniques and augmentation devices. Therefore, there is a need for functionalized scaffold strategies to provide sustained mechanical augmentation during the critical first 12-weeks following repair, and to enhance the healing potential of the repaired tendon and tendon-bone interface. Tissue engineered approaches that combine the use of scaffolds, cells, and bioactive molecules towards promising new solutions for rotator cuff repair are reviewed. The ideal scaffold should have adequate initial mechanical properties, be slowly degrading or non-degradable, have non-toxic degradation products, enhance cell growth, infiltration and differentiation, promote regeneration of the tendon-bone interface, be biocompatible and have excellent suture retention and handling properties. Scaffolds that closely match the inhomogeneity and non-linearity of the native rotator cuff may significantly advance the field. While substantial pre-clinical work remains to be done, continued progress in overcoming current tissue engineering challenges should allow for successful clinical translation.

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Evaluation of a collagen-coated, resorbable fiber scaffold loaded with a peptide basic fibroblast growth factor mimetic in a sheep model of rotator cuff repair

Cited by 25 publications

References 25 publications

Collagen as Coating Material for 45S5 Bioactive Glass-Based Scaffolds for Bone Tissue Engineering

Collagen as Coating Material for 45S5 Bioactive Glass-Based Scaffolds for Bone Tissue Engineering

A prospective study comparing tendon-to-bone interface healing using an interposition bioresorbable scaffold with a vented anchor for primary rotator cuff repair in sheep

Current Status of Tissue-engineered Scaffolds for Rotator Cuff Repair

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