Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

Izquierdo, Luís; Marques, Alexandre F.; Gállego, Montserrat; Sanz, Sílvia; Tebar, Sílvia; Riera, Cristina; Quintó, Llorenç; Aldasoro, Edelweiss; Almeida, Igor C.; Gascón, Joaquim

doi:10.1590/0074-0276130112

Cited by 20 publications

(21 citation statements)

References 21 publications

(40 reference statements)

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“…Although O -glycans of GPI-mucins from the insect-derived parasite forms have been well characterized in several parasite strains and genotypes, the exact structural information of most O -glycans for the mammal-dwelling tGPI-mucins remains unknown. Partial structural analysis and immunoassays have revealed that many of these trypomastigote-derived GPI-mucin (tGPI-mucin) O -glycans contain a terminal α-Gal residue, which is non-reducing and conserved on tGPI-mucins from at least four major Chagas disease causing T. cruzi genotypes [15, 60, 61]. These tGPI-mucin glycans are predominantly branched, and highly heterogeneous with different connectivities of the terminal α-Gal moiety to another sugar unit [12, 62].…”

Section: Resultsmentioning

confidence: 99%

Enhancing glycan isomer separations with metal ions and positive and negative polarity ion mobility spectrometry-mass spectrometry analyses

et al. 2016

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Glycomics has become an increasingly important field of research since glycans play critical roles in biology processes ranging from molecular recognition and signaling to cellular communication. Glycans often conjugate with other biomolecules, such as proteins and lipids, and alter their properties and functions, so glycan characterization is essential for understanding the effects they have on cellular systems. However, the analysis of glycans is extremely difficult due to their complexity and structural diversity (i.e., the number and identity of monomer units, and configuration of their glycosidic linkages and connectivities). In this work, we coupled ion mobility spectrometry with mass spectrometry (IMS-MS) to characterize glycan standards and biologically important isomers of synthetic αGal-containing O-glycans including glycotopes of the protozoan parasite Trypanosoma cruzi, which is the causative agent of Chagas disease. IMS-MS results showed significant differences for the glycan structural isomers when analyzed in positive and negative polarity and complexed with different metal cations. These results suggest that specific metal ions or ion polarities could be used to target and baseline separate glycan isomers of interest with IMS-MS.

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Section: Resultsmentioning

confidence: 99%

Enhancing glycan isomer separations with metal ions and positive and negative polarity ion mobility spectrometry-mass spectrometry analyses

et al. 2016

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“…Previous studies have also proposed a chemiluminescent ELISA (CL-ELISA) with purified trypomastigote glycoproteins for the detection of lytic protective antibodies against T. cruzi in human serum (33,51,52). CL-ELISA achieved high diagnostic accuracy in both areas that are endemic (51,52) and nonendemic (33) for the disease. Detection systems, such as chemiluminescence, increase light amplification and signal duration in comparison with traditional ELISAs.…”

Section: Discussionmentioning

confidence: 99%

Serological Diagnosis of Chronic Chagas Disease: Is It Time for a Change?

et al. 2016

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“…Inconclusive or equivocal results were determined by a titer between 0.9 and 1.0. [ 27 , 35 ]All sera were tested in duplicate and the results were expressed as the mean of two simultaneous determinations.…”

Section: Methodsmentioning

confidence: 99%

Altered Hypercoagulability Factors in Patients with Chronic Chagas Disease: Potential Biomarkers of Therapeutic Response

et al. 2016

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Thromboembolic events were described in patients with Chagas disease without cardiomyopathy. We aim to confirm if there is a hypercoagulable state in these patients and to determine if there is an early normalization of hemostasis factors after antiparasitic treatment. Ninety-nine individuals from Chagas disease-endemic areas were classified in two groups: G1, with T.cruzi infection (n = 56); G2, healthy individuals (n = 43). Twenty-four hemostasis factors were measured at baseline. G1 patients treated with benznidazole were followed for 36 months, recording clinical parameters and performance of conventional serology, chemiluminescent enzyme-linked immunosorbent assay (trypomastigote-derived glycosylphosphatidylinositol-anchored mucins), quantitative polymerase chain reaction, and hemostasis tests every 6-month visits. Prothrombin fragment 1+2 (F1+2) and endogenous thrombin potential (ETP) were abnormally expressed in 77% and 50% of infected patients at baseline but returned to and remained at normal levels shortly after treatment in 76% and 96% of cases, respectively. Plasmin-antiplasmin complexes (PAP) were altered before treatment in 32% of G1 patients but normalized in 94% of cases several months after treatment. None of the patients with normal F1+2 values during follow-up had a positive qRT-PCR result, but 3/24 patients (13%) with normal ETP values did. In a percentage of chronic T. cruzi infected patients treated with benznidazole, altered coagulation markers returned into normal levels. F1+2, ETP and PAP could be useful markers for assessing sustained response to benznidazole.

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Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

Cited by 20 publications

References 21 publications

Enhancing glycan isomer separations with metal ions and positive and negative polarity ion mobility spectrometry-mass spectrometry analyses

Enhancing glycan isomer separations with metal ions and positive and negative polarity ion mobility spectrometry-mass spectrometry analyses

Serological Diagnosis of Chronic Chagas Disease: Is It Time for a Change?

Altered Hypercoagulability Factors in Patients with Chronic Chagas Disease: Potential Biomarkers of Therapeutic Response

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