Evaluation of a cell culture assay for determination of water quality of oil-refinery effluents

Richardson, Des; Dorris, Troy C.; Burks, S.L.; Browne, R. H.; Higgins, M. L.; Leach, Franklin R.

doi:10.1007/bf01691979

Cited by 20 publications

(4 citation statements)

References 9 publications

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“…The use of laboratory cultured animals for detecting the toxicity of pollutants has many advantages (Lee, 1977;Richardson et al, 1977). When certain biological parameters are suggested as indicators for toxicity thresholds of hydrocarbons, they should be sensitive, and the bioassay procedures adopted should also last for a short interval.…”

Section: Discussionmentioning

confidence: 99%

Changes in nesting behavior and lipid content of a marine amphipod (Amphithoe valida) to the toxicity of a no. 2 fuel oil

Lee¹,

Macko²,

Nicol³

1981

Water Air Soil Pollut

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Laboratory cultured amphipods, Amphithoe valida, were exposed to the water soluble fractions (WSF) of a No. 2 fuel oil for 6 days, and then transferred to clean sea water for one week. Survival and nesting behavior were observed daily, and the lipid contents were checked at the end of exposure and at the end of depuration. Survival of amphipods during exposure was high at all concentrations of W S F tested (0 to 25%). A delayed toxicity was observed; mortality was high in concentrations /> 15% WSF during depuration. The number of nests decreased with increasing concentration of WSF and length of exposure. Recovery of nest building activity in clean sea water was either lacking or small, indicating some damage to their nesting capability. The lipid contents of amphipods were close to the expected range (except at 25~ WSF) following the exposure, but dropped to about 1% following recovery compared with an expected value of 3.3%. Impairments of the chemosensory and locomotory systems may occur during exposure, which prevent amphipods from constructing nests for protection and food reserves, and eventually lead animals to use their stored energy of lipid for survival. Of the three biological parameters studied, nesting behavior and lipid content as a percentage of dry weight were better indicators of the sublethal stress of WSF than the response of survival; significant alterations of the former two parameters were found at the concentrations /> 5% WSF.Water, Air, and Soil Pollution 15 (1981) 185-195.

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Section: Discussionmentioning

confidence: 99%

Changes in nesting behavior and lipid content of a marine amphipod (Amphithoe valida) to the toxicity of a no. 2 fuel oil

Lee¹,

Macko²,

Nicol³

1981

Water Air Soil Pollut

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“…In light of the central role of the fresh water environment in receiving, accumulating and distributing potentially hazardous substances, it was not surprising that the assessment of water quality soon emerged as another important area of application. Richardson et al (1977) evaluated a mammalian cell culture assay for estimating the water quality of oil-refinery effluents. Several studies involving mammalian cells were performed by health laboratories and water boards (reviewed in Hunt et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish

2006

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“…The ability of cultured cells to assess toxicity of waste waters (Halder and Ahne, 1990) makes them a very profitable screening tool to assay water quality (Richardson et al, 1977;Hunt et al, 1986) and to detect organic toxic compounds (Tarazona et al, 1990). In this case, the use of rainbow trout cells, one of the cultured species in the area, also allows risk assessment of the fish canning effluents, particularly when it is difficult to know the dilution factor and the persistence of the toxic compounds present in the effluents, when reaching the culturing cages and the mussel platforms.…”

Section: Discussionmentioning

confidence: 99%

Assessing organic toxic pollutants in fish-canning factory effluents using cultured fish cells

Vega¹,

Castaño

Blazquez³

et al. 1994

Ecotoxicology

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The fibroblastic RTG-2 fish cell line has been used to assess the cytotoxicity of organic compounds present in the effluents discharged by a canning factory. Organic compounds of the effluents were extracted and concentrated, and their cytotoxicity determined by measuring three end points: the cellular mass, the cell viability and the intracellular ATP content of the cells. Three out of the four organic concentrates were found to be highly cytotoxic, and these three toxic concentrates were fractionated, into 210 organic fractions, using an HPLC system. Up to six toxic fractions, present in all three organic concentrates, could be detected. Thus, when assessing the environmental effect of fish-canning factory effluents, the presence of specific toxic chemicals must be considered, in addition to toxicological problems due to fish-offal sewage.

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Evaluation of a cell culture assay for determination of water quality of oil-refinery effluents

Cited by 20 publications

References 9 publications

Changes in nesting behavior and lipid content of a marine amphipod (Amphithoe valida) to the toxicity of a no. 2 fuel oil

Changes in nesting behavior and lipid content of a marine amphipod (Amphithoe valida) to the toxicity of a no. 2 fuel oil

Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish

Assessing organic toxic pollutants in fish-canning factory effluents using cultured fish cells

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