Evaluation of a bulk-milk ELISA test for the classification of herd-level bovine leukemia virus status

Sargeant, Jan M.; Kelton, D.F.; Martin, S W; Mann, Elroy D.

doi:10.1016/s0167-5877(96)01139-7

Cited by 14 publications

(5 citation statements)

References 9 publications

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“…Most of these ELISA have only been evaluated by comparisons with the AGID test, which is significantly less sensitive than the RIP assay. [3][4][5][6][7] The performance of ELISA for detecting BLV antibodies in samples of pooled milk has been evaluated in herds whose cattle had been characterized by the poorly sensitive AGID 10,[15][16][17] or in pooled samples made by artificially mixing samples from each cow. 8 Unlike other ELISA, the blocking ELISA 108 described here has been critically compared with the RIP assay in a relatively large number of serum samples from cattle of various ages, breeds, and geographic locations as well as from cattle belonging to herds with various rates of BLV infection and cattle in early stages of infection.…”

Section: Discussionmentioning

confidence: 99%

“…Several ELISA have been used for the detection of BLV antibodies in bulk-tank milk samples. 8,10,[15][16][17] In the study reported here, we described a new blocking ELISA for the detection of BLV antibodies in serum and milk samples of cattle. Sensitivity of this ELISA was critically evaluated in a large number of serum samples from characterized cattle of various origins whose BLV status had been defined by use of the RIP assay.…”

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confidence: 99%

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Development and evaluation of a highly sensitive and specific blocking enzyme-linked immunosorbent assay and polymerase chain reaction assay for diagnosis of bovine leukemia virus infection in cattle

Gutiérrez¹,

Dolcini²,

Arroyo³

et al. 2001

ajvr

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Development and evaluation of a highly sensitive and specific blocking enzyme-linked immunosorbent assay and polymerase chain reaction assay for diagnosis of bovine leukemia virus infection in cattle

Gutiérrez¹,

Dolcini²,

Arroyo³

et al. 2001

ajvr

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“…The use of tests adapted for use with bulk milk samples is of interest because of the potential cost savings and the possibility for the automation of testing. Tests adapted for use with bulk milk have been developed for several bovine diseases, such as those caused by Brucella abortus (22), bovine leukemia virus (19), bovine viral diarrhea virus (15), Leptospira hardjo (1), bovine herpesvirus type 1 (24), Mycobacterium avium subsp. paratuberculosis (14), bovine corona virus (23), bovine respiratory syncytial virus (2), Coxiella burnetii (16), Ostertagia spp.…”

mentioning

confidence: 99%

Evaluation of Two Enzyme-Linked Immunosorbent Assays for Detecting Salmonella enterica subsp. enterica Serovar Dublin Antibodies in Bulk Milk

Veling

Zijderveld²,

Bemmel³

et al. 2001

Clin Diagn Lab Immunol

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Two enzyme-linked immunosorbent assays (ELISAs) for the detecting Salmonella enterica subsp. enterica serovar Dublin antibodies in bulk milk were developed and evaluated for potential use in control programs. The ELISAs were based on either lipopolysacharide (LPS ELISA) or flagellar antigen (GP ELISA). Sensitivity was determined with 79 case herds with a wide range of clinical signs. Specificity was determined with 125 Dutch and 200 Swedish control herds. The relation between antibodies in bulk milk, antibodies in serum, and the level of milk production of individual cows was studied with 61 case herds. The optimal optical density (OD) values of the LPS ELISA and the GP ELISA were determined to be 0.2 and 0.5, respectively. The sensitivities of the LPS ELISA and the GP ELISA were 54 and 63%, respectively, with a specificity of 98% for both ELISAs with samples from the Dutch control herds. The specificities for samples from the Swedish herds were 100% for the LPS ELISA and 95% for the GP ELISA. The sensitivity of the combination of tests was 65% when samples were run in parallel, and the specificity was 100% when samples were run in series, irrespective of whether the samples came from Dutch or Swedish control herds. The variance (R 2 ) in the OD value for bulk milk samples could be explained by the percentage of seropositive lactating cows in a herd with the LPS ELISA for 51% of the samples and with the GP ELISA for 72%. The variance in the OD value was best explained by the combination of the percentage of seropositive lactating cows in the herd and the mean log 10 serum antibody titer for that herd (R 2 ‫؍‬ 62% for the LPS ELISA and R 2 ‫؍‬ 75% for the GP ELISA). Case herds more often tested negative by the ELISA with bulk milk when the percentage of seropositive lactating cows was less than 5%. It is concluded that both ELISAs with bulk milk can be used in control programs to distinguish between infected and noninfected herds. Specificity can be increased by using the two tests in combination. Sensitivity was relatively low for both single tests and both tests combined.

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“…The bulk tank milk ELISA is useful for identification of herds which are negative for BLV infection. Since the specificity of the ELISA test for milk was moderately low, herds identified as positive by the ELISA would require further testing at the individual or herd level to definitively establish their BLV status (24). The sensitivity and specificity of the milk ELISA is estimated to be adequate until the prevalence of BLVinfected individuals in the country is less than 1% (2).…”

Section: Discussionmentioning

confidence: 99%

A Serological Study on Bovine Leukemia Virus Infection in Ten Provinces of Iran between 2010 and 2012

et al. 2012

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Background and Aims: Bovine leukaemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus of the family Retroviridae. BLV is the causative agent of enzootic bovine leukaemia and infects cattle worldwide, imposing economic impact on the dairy cattle industry. The purpose of this study was to estimate the seroprevalence of BLV in cattle in some provinces of Iran. Materials and Methods: A total of 280 cows over 2 years old from 10 provinces of Iran in different regions and environments from industrial and less industrial herds were used in the study. Blood samples from all cows were taken both with and without EDTA. Serum separation for the ELISA test and leukocyte count, were performed upon receipt without delay. Cattle without fever that had lymphocyte numbers of more than 9,000/μl were suspected to have persistent lymphocytosis (PL). Sera samples were examined for antibodies against BLV by blocking ELISA. Results: The seroprevalence of BLV among animals was 32.8% and among provinces was 80%. Seropositive cattle had higher total leukocyte and lymphocyte count and lower neutrophil count than seronegative cattle (P<0.001). Among BLV seropositive animals, the rate of PL was 36.9%. None of the seronegative animals showed lymphocytosis. Conclusion: Comparing the data with previous studies on seroprevalence of BLV in different localities in Iran, the prevalence of the infection has been raised. These results suggest that promoting control programs in Iran are very important. Furthermore, it will be essential to conduct nationwide surveillance program and determine the major risk factors.

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Evaluation of a bulk-milk ELISA test for the classification of herd-level bovine leukemia virus status

Cited by 14 publications

References 9 publications

Development and evaluation of a highly sensitive and specific blocking enzyme-linked immunosorbent assay and polymerase chain reaction assay for diagnosis of bovine leukemia virus infection in cattle

Development and evaluation of a highly sensitive and specific blocking enzyme-linked immunosorbent assay and polymerase chain reaction assay for diagnosis of bovine leukemia virus infection in cattle

Evaluation of Two Enzyme-Linked Immunosorbent Assays for Detecting Salmonella enterica subsp. enterica Serovar Dublin Antibodies in Bulk Milk

A Serological Study on Bovine Leukemia Virus Infection in Ten Provinces of Iran between 2010 and 2012

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