Evaluation of a bioartificial liver based on a nonwoven fabric bioreactor with porcine hepatocytes in pigs

Li, Lan Juan; Du, Wei Bo; Zhang, Yi Min; Li, Jun; Pan, Xiao; Chen, Jiajia; Cao, Hong; Chen, Yu; Chen, Yue Mei

doi:10.1016/j.jhep.2005.08.006

Cited by 27 publications

(19 citation statements)

References 58 publications

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“…The former includes hepatic ischemia and hepatectomy, and the latter are based on administering drugs and toxins, such as acetaminophen, D-galactosamine, azoxymethane, concanavalin A, and thioacetamide [37,38]. Our study adopted a widely accepted ALF porcine model induced by D-galactosamine [39,40] because this model demonstrates high reproducibility and potential reversibility [30][31][32]41]. Notably, this pig model was highly similar to both clinical and laboratory indices of patients with drug-induced ALF and provided a suitable model to evaluate the efficacy of our novel Li-ALS treatment.…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of coupled low-volume plasma exchange with plasma filtration adsorption in treating pigs with acute liver failure: A randomised study

Zhou¹,

Li²,

Zhang³

et al. 2015

Journal of Hepatology

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Section: Discussionmentioning

confidence: 99%

“…ALF was induced with D-galactosamine, as described previously by our laboratory [29][30][31][32]. Animals were fasted for 8 hours prior to catheterisation.…”

Section: D-galactosamine-induced Alf Modelmentioning

confidence: 99%

Efficacy of coupled low-volume plasma exchange with plasma filtration adsorption in treating pigs with acute liver failure: A randomised study

Zhou¹,

Li²,

Zhang³

et al. 2015

Journal of Hepatology

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“…An auricular vein was secured and 2.5 mg/kg/h diprivan (AstraZeneca, Caponago, Italy) administered to achieve continuous anaesthesia. Catheterization was performed as described previously [32]. Six hours after the operation, acute liver injury in the pigs was induced by administration of D-galactosamine (GalN, Hanhong Chemical Co., Ltd, Shanghai, China) in 5% (w/v) glucose (pH 6.8) at a dose of 1.5 g/kg body weight via a venous catheter.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure

Cao

Yang

et al. 2012

BMC Med

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110

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BackgroundStem cell-based therapy to treat liver diseases is a focus of current research worldwide. So far, most such studies depend on rodent hepatic failure models. The purpose of this study was to isolate mesenchymal stem cells from human placenta (hPMSCs) and determine their therapeutic potential for treating Chinese experimental miniature pigs with acute liver failure (ALF).MethodshPMSCs were isolated and analyzed for their purity and differentiation potential before being employed as the donor cells for transplantation. ALF models of Chinese experimental miniature pigs were established and divided into four groups: no cell transplantation; hPMSCs transplantation via the jugular vein; X-ray-treated hPMSCs transplantation via the portal vein; and hPMSCs transplantation via the portal vein. The restoration of biological functions of the livers receiving transplantation was assessed via a variety of approaches such as mortality rate determination, serum biochemical analysis, and histological, immunohistochemical, and genetic analysis.ResultshPMSCs expressed high levels of CD29, CD73, CD13, and CD90, had adipogenic, osteogenic, and hepatic differentiation potential. They improved liver functions in vivo after transplantation into the D-galactosamine-injured pig livers as evidenced by the fact that ALT, AST, ALP, CHE, TBIL, and TBA concentrations returned to normal levels in recipient ALF pigs. Meanwhile, histological data revealed that transplantation of hPMSCs via the portal vein reduced liver inflammation, decreased hepatic denaturation and necrosis, and promoted liver regeneration. These ameliorations were not found in the other three groups. The result of 7-day survival rates suggested that hPMSCs transplantation via the portal vein was able to significantly prolong the survival of ALF pigs compared with the other three groups. Histochemistry and RT-PCR results confirmed the presence of transplanted human cells in recipient pig livers (Groups III, IV).ConclusionsOur data revealed that hPMSCs could not only differentiate into hepatocyte-like cells in vitro and in vivo, but could also prolong the survival time of ALF pigs. Regarding the transplantation pathways, the left branch of the portal vein inside the liver was superior to the jugular vein pathway. Thus, hPMSCs transplantation through the portal vein by B-ultrasonography may represent a superior approach for treating liver diseases.

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“…AHF is associated with a mortality of as high as 70-90% despite the great development in internal medicine [1]. Liver transplantation has evident clinic efficacy on AHF, but more than 2/3 of patients with AHF die before a liver is available for transplantation because of a critical shortage of organ donors [2]. In such a situation, the artificial liver support system (ALSS) provides a transitional therapy for patients suffering from AHF [3].…”

Section: Introductionmentioning

confidence: 99%

“…Since 1990s', the researchers in college of medicine, Zhejiang University have made remarkable achievements in ALSS field [2,4]. In resent years, the HALSS, which combines the plasma absorption system and BALSS and is considered to possess all advantages of two other types of ALSS, has become the focus of ALSS research [1,6].…”

Section: Introductionmentioning

confidence: 99%

Development of Control System for Assisted Circulation Apparatus of Artificial Liver Support System

Pan

Chen

et al. 2009

2009 2nd International Conference on Biomedical Engineering and Informatics

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An advanced control system used in assisted circulation apparatus of artificial liver support system was developed based on embedded platform and field bus. This system consisted of a cardiopulmonary bypass-controlling system, a blood circulation and reactors status-monitoring system, and a human-machine interface and communication system. Multiple functions are implemented such as on-line monitoring, controlling and driving of the assisted circulation apparatus, real-time supervising and regulating of the physiological environments in reactors, and convenient, rapid operation mode, with high security, stability and practicality. The implementation of this system is helpful to the independent construction and evaluation of artificial liver support system, as well as the research works on hepatocyte culture and bioreactor design.

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Evaluation of a bioartificial liver based on a nonwoven fabric bioreactor with porcine hepatocytes in pigs

Cited by 27 publications

References 58 publications

Efficacy of coupled low-volume plasma exchange with plasma filtration adsorption in treating pigs with acute liver failure: A randomised study

Efficacy of coupled low-volume plasma exchange with plasma filtration adsorption in treating pigs with acute liver failure: A randomised study

Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure

Development of Control System for Assisted Circulation Apparatus of Artificial Liver Support System

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