Evaluation of a <i>Brassica napus</i> Auxin-Repressed Gene Induced by Flea Beetle Damage and <i>Sclerotinia sclerotiorum</i> Infection

Abstract

Biotic stresses negatively affect canola growth and production. Flea beetle damage and Sclerotinia sclerotiorum (S. sclerotiorum) infection are two of the worst biotic stresses for canola. Auxin Repressed Proteins (ARPs) responsive to several abiotic stresses have been reported. However, information about ARPs induced by Flea beetle damage and S. sclerotiorum infection, their roles in biotic stress tolerance are still lacking in canola. ESTs for an Auxin Repressed Protein 1 (BnARP1) were highly represented (expressed) in a Brassica napus subtractive library developed after leaf damage by the crucifer flea beetle (Phyllotreta cruciferae). Expression of this gene was under different developmental control in B. napus, and it was co-induced in B. napus by flea beetle feeding, S. sclerotiorum infection, drought and cold. A total of 25 BnARP genes were represented in different B. napus stress and development EST libraries and indicated larger, diversified families than known earlier. Dwarf phenotypes, primary root growth inhibition, lateral root enhancement, reduced sensitivity to 2, 4-D, and reduced PIN1 and LOX expression in transgenic Arabidopsis expression lines suggest that BnARP1 is an auxin repressor that prevents auxin transport and supports an interaction between the auxin and jasmonate signalling pathways. And the increased survival after S. sclerotiorum infection in transgenic over-expression Arabidopsis suggests that BnARP1 could play a role in S. sclerotiorum tolerance through connecting auxin and jasmonate signalling pathways.

Global Dynamics in Protein Disorder during Maize Seed Development

“…Evidence has emerged for ARP family members as IDPs. ARP genes are responsive to hormones involved in responses to biotic stress, such as salicylic acid (SA) and methyl jasmonate (MeJA), as well as to hormones that regulate plant growth and development, including auxins [72,73,74]. Calreticulin (CALR) is well recognized as a Ca 2+ -binding protein molecular chaperone that assists the folding of newly synthesized glycoproteins and modulates the Ca 2+ homeostasis in the endoplasmic reticulum (ER) lumen [75].…”

Genome-wide identification and expression analysis of dormancy-associated gene 1/auxin repressed protein (DRM1/ARP) gene family in Glycine max

“…On the other hand, PpARP1 and PpARP2 genes are induced by 0.2mM of IAA in Pyrus pyrifolia (Shi et al, 2013). In Brassica napus, auxin 2,4-Dichlorophenoxyacetic acid strongly induces the accumulation of BnARP1 transcripts after 2 hours of treatment in transgenic lines of Arabidopsis overexpressing BnARP1 (Wu et al, 2017).…”

Proteomic analysis of near‐isogenic lines reveals key biomarkers on wheat chromosome 4B conferring drought tolerance

“…Likewise in our previous transcriptomics study with the same NILs, we observed the downregulation of all the disease‐resistance genes in the tolerant isolines when subjected to DS (Nouraei et al., 2022). Although several ARPs responsive to various abiotic stresses have been reported, their function in stress adaptation is less well known (L. Wu et al, 2017).…”

“…Proteins play an essential role in the plant's phenotype by direct involvement in cell structure and metabolism (Kosová et al., 2018). Because proteins are involved in plant stress responses, proteomic studies can lead to an understanding of the possible relationships between protein changes and plant stress tolerance (S. Wu et al, 2017). Near‐isogenic lines (NILs) are lines with identical genetic backgrounds except for a few specific locations that are used to minimize genetic background effects such as epistatic interactions (Wang et al., 2021).…”