Evaluation of 5 rapid colorimetric and immunochromatographic tests for the detection of carbapenemases in Pseudomonas aeruginosa and Acinetobacter spp. isolates

Fournier, Damien; Triponney, Pauline; Gabriel, Lorrant Cavanha; Plésiat, Patrick; Jeannot, Katy; Potron, Anaïs

doi:10.1007/s10096-023-04631-8

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…Since the activity of the last-line antibiotics, such as the newly developed cefiderocol, depend on the type of carbapenemase, rapid detection of these carbapenemase-producing bacteria has become crucial for optimizing antibiotherapy in case of infection and implementing appropriate hygiene measures (6). In recent years, several rapid immunochromatographic tests (Coris BioConcept, NG Biotech) have been developed for the detection of carbapenemase-producing Enterobacterales ; but none has been specifically designed for non-fermenting Gram-negative bacilli, with the exception of the Coris OXA-23 K-SET test (accurate detection of OXA-23 group carbapenemases) (7)(8)(9). The novel RESIST ACINETO test (Coris BioConcept) has recently been developed to detect the main carbapenemases identified in the Acinetobacter species, namely the OXA-23-, OXA-24/40-, OXA-58and NDM-groups (10).…”

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confidence: 99%

RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture inAcinetobacterspecies

Potron,

Daniel,

Bay

et al. 2024

Preprint

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The immunochromatographic assay RESIST ACINETO (Coris BioConcept) was evaluated on a collection of 121 Acinetobacter spp. clinical isolates, including 104 carbapenemase producers. The strains producing carbapenemases OXA-23, -40, -58, or/and NDM were accurately detected from bacterial cultures and directly from blood cultures, with the exception of an OXA-23/NDM-1-positive A. radioresistens isolate (only detected through standard culture). The performance of the RESIST ACINETO test was excellent (sensitivity 99%, specificity 100%) on this collection of isolates.

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confidence: 99%

RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture inAcinetobacterspecies

Potron,

Daniel,

Bay

et al. 2024

Preprint

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RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture in Acinetobacter species

Potron,

Daniel,

Bay

et al. 2024

Microbiol Spectr

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Carbapenem-resistant Acinetobacter baumannii (CRAB) are increasingly reported worldwide and a leading cause of mortality associated with antimicrobial resistance. Their early detection, particularly in the cases of bloodstream infections, is crucial in attempting to initiate effective antibiotic treatment. The immunochromatographic assay RESIST ACINETO (Coris BioConcept) is a new test developed for the detection of OXA-23, OXA-40/58, and New-Delhi Metallo-beta-lactamase (NDM) carbapenemases in Acinetobacter spp. We evaluated this test on a collection of 121 Acinetobacter spp. clinical isolates, including 104 carbapenemase producers (97 carbapenemases targeted by the test) and 17 non-carbapenemase producers. The performance of the RESIST ACINETO test was evaluated according to the manufacturer’s recommendations from bacterial and blood cultures. The strains producing the carbapenemases OXA-23, -40, -58, or/and NDM were accurately detected from bacterial cultures and directly from blood cultures, with the exception of one OXA-23/NDM-1-positive Acinetobacter radioresistens isolate (only detected through standard culture). None of the non-carbapenemase producers tested positive. The RESIST ACINETO test demonstrated sensitivity/specificity of 100%/100% and 99%/100% on bacterial and blood cultures, respectively. IMPORTANCE The incidence of bloodstream infections with carbapenem-resistant Acinetobacter baumannii (CRAB) could be very high in some countries such as the Balkans or Southeast Asia. In case of positive blood cultures with Gram-negative bacteria, the use of the RESIST ACINETO test could prove highly beneficial for the rapid identification of these imipenem-resistant bacteria and their antibiotic resistance mechanisms. In addition, it is now well established that New-Delhi Metallo-beta-lactamase (NDM) carbapenemase-producing isolates can have increased MICs of cefiderocol, which is an alternative treatment for these infections. This test may also allow the optimization of treatment based on the type of carbapenemase present. Finally, the RESIST ACINETO test is a rapid, easy-to-use, and cost-effective assay that demonstrates excellent performance in detecting the major acquired carbapenemases present in the Acinetobacter species .

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Evaluation of 5 rapid colorimetric and immunochromatographic tests for the detection of carbapenemases in Pseudomonas aeruginosa and Acinetobacter spp. isolates

Cited by 2 publications

References 7 publications

RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture inAcinetobacterspecies

RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture inAcinetobacterspecies

RESIST ACINETO test for the rapid detection of NDM and OXA acquired carbapenemases directly from blood culture in Acinetobacter species

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