Evaluation of 5 new media containing extracts of seeds applied to Candida dubliniensis screening

Loreto, Érico S.; Bolzan, Aline Adriana; Linares, Carlos Eduardo Blanco; Boff, Everton; Santurio, Jânio M.; Alves, Sydney Hartz

doi:10.1016/j.diagmicrobio.2006.01.009

Cited by 15 publications

(9 citation statements)

References 19 publications

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“…However, variations in the composition of these agars, and the use of different proposed media, such as tobacco, sesame, and linseed media (22,23), have shown similar results ( Table 1). On the other hand, caffeic acid-ferric citrate and casein agar are not advantageous if compared with these (10,24).…”

Section: Discussionsupporting

confidence: 78%

Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar

Loreto

Pozzatti

Scheid

et al. 2008

Clinical Laboratory Analysis

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Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans and therefore, may be misidentified in microbiological laboratories. Because molecular methods can be onerous and unfeasible in routine mycological laboratories with restricted budgets such as those in developing countries, phenotypic techniques have been encouraged in the development of differential media for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of 30 C. dubliniensis isolates and 100 C. albicans isolates on two new proposed media: rosemary (Rosmarinus officinalis) extract agar (REA) and oregano (Origanum vulgare) extract agar (OEA). These substrates are traditionally used as spices and medicinal herbs. In both of these media, all C. dubliniensis isolates (100%) showed rough colonies with peripheral hyphal fringes and abundant chlamydospores after 24 to 48 hr of incubation at 25 degrees C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphal fringes or chlamydospores. In conclusion, REA and OEA offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.

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Section: Discussionsupporting

confidence: 78%

Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar

Loreto

Pozzatti

Scheid

et al. 2008

Clinical Laboratory Analysis

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“…When we discovered that C. dubliniensis forms chlamydospores on Staib agar, we also noted the ability of pure G. abyssinica plant seed material, the main constituent of Staib agar, to induce chlamydospores in C. dubliniensis . The capacity to induce chlamydospore formation in C. dubliniensis was found for many, but not all, other plant seeds tested, 63 and our own unpublished results. This observation supports the view that, apart from nutritional requirements and incubation parameters, chlamydospore induction is also positively influenced by specific external signals.…”

Section: Induction Germination and Isolation Of Chlamydosporesmentioning

confidence: 54%

Chlamydospore formation in Candida albicans and Candida dubliniensis– an enigmatic developmental programme

Staib

Morschhäuser

2006

Mycoses

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Chlamydospore formation has served for a long time for identification of the human fungal pathogen Candida albicans, but the biological function of these structures still remains a secret. They have been proposed to allow survival in harsh environmental conditions, but this assumption remains to be proven. Chlamydospores are produced only by the two closely related species C. albicans and Candida dubliniensis, whose natural habitats are humans and warm-blooded animals, but not by other Candida species that are also found outside animal hosts. However, no role in the pathogenesis of Candida infections has been assigned to these unusual cells and only a limited number of studies have been conducted in the past to unravel their function. The development of new molecular tools and the recent discovery of mating in C. albicans have also restimulated investigations to understand the morphogenesis and function of chlamydospores. The finding that chlamydospore formation is differentially controlled by certain environmental signals in C. albicans and C. dubliniensis has opened new approaches to study the regulation of this morphogenetic programme. These studies have already identified genes and signalling pathways that are required for chlamydospore production and should lead to a detailed understanding of this fascinating developmental process.

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“…This species had previously been reported in South Africa, Egypt and Tunisia as well as Ghana (Al Mosaid et al, 2003;Bii et al, 2009;JabraRizk et al, 2001;Kwamin et al, 2010). This may be due to, in part, the type of samples used in the study; urine and vaginal specimens have been reported to have a low incidence of C. dubliniensis (Loreto et al, 2006).…”

Section: Discussionmentioning

confidence: 90%

An investigation of the distribution of Candida species in genitourinary candidiasis and pelvic inflammatory disease from three locations in Ghana

Hale¹,

Garrill²

2014

Afr. J. Microbiol. Res.

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The aim of this study was to determine the frequency of various Candida species in cases of genitourinary candidiasis and pelvic inflammatory disease (PID) in Ghana. To achieve this, Candida isolates were recovered from high vaginal swabs of patients with vulvovaginal candidiasis (VVC), urine samples from patients with urinary tract infections (UTIs) and endocervical swabs from patients with PID, from three teaching hospitals in Ghana. The hospitals were located at Korle Bu, Komfo Anokye and Tamale. These isolates were identified to the species level on the basis of the color of the colonies that they formed on chromogenic medium. Candida albicans was the most common species in high vaginal swabs from patients present for the first time with VVC in each of the three locations, and was present in 53.4% of the total swabs. The other species that were present were Candida glabrata (21.6%), Candida parapsilosis (15.5%), Candida tropicalis (4.7%) and Candida krusei (4.7%). Only single species were found in these swabs. In patients with VVC for at least the third time, Chi square analysis indicated that the frequency of each of these species were not statistically different from those present for the first time, although 15% of the swabs from these patients contained more than one species. For all patients with VVC, no significant differences were observed in the frequencies of the species between the three locations. Similar distributions were found in swabs taken from patients with PID. Across the three locations however, there was a significant difference in the frequency of C. albicans, which was present in 68 and 69.6% of patients from Komfo Anokye and Tamale, but only 26.7% of patients from Korle Bu. Twenty one percent of swabs from patients with PID contained more than one species. Urine samples were also collected from two of the locations, Korle Bu and Tamale, in female patients with candiduria. In Korle Bu, C. glabrata was the most prominent species (37.8%) followed by C. albicans (22.4%), C. parapsilosis (21.7%), C. tropicalis (10.5%), C. krusei (7%) and Candida lusitaniae (0.7%). In Tamale, the species distribution was C. albicans (60.9%), C. glabrata (21.7%), C. parapsilosis (13%) and C. krusei (4.3%). Statistical analysis indicated a significant difference in the frequency of C. albicans between the two locations. Fourteen percent of the urine samples contained more than one species. Taken as a whole, these data highlight a relatively high prevalence of species other than C. albicans, in cases of genitourinary candidiasis and PID in Ghana. This is consistent with a trend towards the emergence of other Candida species that may be more resistant to the first line antifungal treatments.

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Evaluation of 5 new media containing extracts of seeds applied to Candida dubliniensis screening

Cited by 15 publications

References 19 publications

Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar

Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar

Chlamydospore formation in Candida albicans and Candida dubliniensis– an enigmatic developmental programme

An investigation of the distribution of Candida species in genitourinary candidiasis and pelvic inflammatory disease from three locations in Ghana

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