Evaluation of 4 Presurgical Skin Preparation Methods in Mice

Abstract

Mice routinely undergo surgical procedures for use in research; however, studies of skin preparation methods to achieve antisepsis are rare. The present study evaluated 4 skin preparation treatments: depilatory agent followed by povidone-iodine and alcohol scrub; depilatory agent followed by povidone-iodine and saline scrub; electric clippers followed by povidoneiodine and alcohol scrub; and electric clippers followed by povidone-iodine and saline scrub. Swabs for bacterial culture were obtained immediately after hair removal and after scrubbing to measure the reduction in bacterial load. Full-thickness incisions were assigned ASEPSIS wound scores and examined histologically on days 0, 1, and 7 after surgery. Neither bacterial load growth nor ASEPSIS wound scores differed among any of the treatments. Histopathology revealed statistically significant but biologically irrelevant differences. Overall all treatment methods achieved acceptable bacterial load reduction and surgical site healing.

Dialdehyde Nanocrystalline Cellulose as Antibiotic Substitutes against Multidrug-Resistant Bacteria

“…The pathological sections were obtained by Gram staining. The histological assessment was conducted by scoring pathological sections based on the criteria, including epidermal lesion, dermal inflammation, and cell proliferation. , For the first two criteria, the scores ranged from 0 (severe) to 3 (absent). For the criterion of cell proliferation, the scores ranged from 0 (absent) to 3 (prominent).…”

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

“…The preoperative skin preparation includes hair removal and disinfection of the surgical sites. It has been demonstrated that different disinfection methods indicated no significant differences, irrespective of depilatory agents or electric clippers depilation methods used [ 8 , 9 ]. However, in previous experiments, we have found fortuitously that mice depilated by sodium sulfide aqueous solution and scissors hair cutting methods presented some problems in the stages of wound healing.…”

“…Some studies suggested that using clippers or depilatory cream probably results in few SSIs, compared with shaving using a razor [ 13 , 14 , 15 ]. However, some studies also indicated that both electric razor clipping and depilatory agents are safe in rats and mice [ 9 , 16 ]. Chemical depilatory can break the disulfide linkages on the keratin protein backbone by enhancing aqueous solubility, leading to hair removal [ 17 ].…”

Description of non‐brachycephalic canine conjunctival microbiome before and after application of an antiseptic preparation

“…The goal of surgical preparation is to reduce commensal bacterial load in order to prevent surgical site infections and postoperative complications such as bacterial endophthalmitis 1–6 . Surgical preparation practices in veterinary medicine are derived from human protocols identifying reductions in commensal bacteria following use of common antiseptics including chlorhexidine gluconate, povidone‐iodine, and hexachlorophene 7–11 .…”

“…The goal of surgical preparation is to reduce commensal bacterial load in order to prevent surgical site infections and postoperative complications such as bacterial endophthalmitis. [1][2][3][4][5][6] Surgical preparation practices in veterinary medicine are derived from human protocols identifying reductions in commensal bacteria following use of common antiseptics including chlorhexidine gluconate, povidone-iodine, and hexachlorophene. [7][8][9][10][11] These protocols have demonstrated similar reductions in bacterial load when used in veterinary medicine utilizing chlorhexidine gluconate, chlorhexidine diacetate, and povidone-iodine antiseptics.…”