Evaluation of 20(S)-ginsenoside Rg3 loaded hydrogel for the treatment of perianal ulcer in a rat model

Jin, Longhai; Liu, Jinping; Wang, Shu; Zhao, Linxian; Li, Jiannan

doi:10.1016/j.jgr.2022.03.002

Cited by 8 publications

(8 citation statements)

References 41 publications

(50 reference statements)

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“…Given the origin of the isolated macrocyclic trichothecenes from the highly toxic mushroom P. cornu-damae and the known potent cytotoxic properties of trichothecene analogues, we also conducted in vitro cytotoxicity evaluations of all the isolated compounds (1-4) in this study. The cytotoxicity was assessed against four human-derived cancer cell lines, SK-OV-3, SK-MEL-2, A549, and HCT15, using the SRB bioassay, [15][16][17][18][19][20] with doxorubicin serving as the positive control. As shown in Table 2, 12′-episatratoxin H (3) exhibited potent cytotoxic effects against all four cell lines tested, with IC 50 values in the range of 0.7-2.8 nM, which were stronger than that of doxorubicin, and satratoxin H (2) showed moderate cytotoxic potency against all four cell lines, with IC 50 values in the range of 1.93-4.22 µM, whereas N-hydroxy-Phe-Phe (1) and roridin F (4) did not show any significant cytotoxicity (IC 50 , >10.0).…”

Section: Cytotoxicity Of Compounds 1-4mentioning

confidence: 99%

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

Lee,

Jung,

Ryoo

et al. 2023

Org. Biomol. Chem.

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Section: Cytotoxicity Of Compounds 1-4mentioning

confidence: 99%

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

Lee,

Jung,

Ryoo

et al. 2023

Org. Biomol. Chem.

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“…Given the origin of the mixture (compounds 1 and 2 ) from the highly toxic mushroom P. cornu-damae , we proceeded to conduct additional in vitro cytotoxicity evaluations to explore the synergistic effect between compounds 1 and 2 in this study. The evaluation of cytotoxicity was conducted on four human-derived cancer cell lines (A549, SK-MEL-2, HCT15, and SK-OV-3) using the SRB bioassay [ 34 , 35 ], with doxorubicin employed as the positive control. However, we did not detect significant cytotoxicity (IC 50 > 30.0).…”

Section: Resultsmentioning

confidence: 99%

“…To assess the mixture’s cytotoxicity against four cancer cell lines, we employed a sulforhodamine B (SRB) bioassay [ 34 , 35 ]. The utilized cell lines in this investigation included (1) skin melanoma, SK-MEL-2, (2) ovarian malignant ascites, SK-OV-3, (3) non-small cell lung carcinoma, A549, and (4) colon adenocarcinoma, HCT.…”

Section: Methodsmentioning

confidence: 99%

Determination of the Absolute Configuration of Secondary Alcohols in a Compound Mixture via the Application of Competing Enantioselective Acylation Coupled with LC/MS Analysis

Lee,

Kim,

Baek

et al. 2024

Pharmaceutics

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The determination of natural product stereochemistry plays a significant role in drug discovery and development. Understanding the stereochemistry of natural products is essential for predicting and optimizing their interactions with biological targets, which, in turn, influences their therapeutic efficacy, safety, and overall impact on living organisms. Here, we present the first application of competitive enantioselective acylation (CEA) reactions in conjunction with LC/MS analysis for determining the absolute configuration of secondary alcohols in natural products which were purified as a mixture. This approach utilizes the enantiomeric pair of HBTM (homobenzotetramisole) catalysts, demonstrating sufficient kinetic resolution for the acylation of secondary alcohols. The rapid reaction kinetics were quantitatively estimated with LC/MS analysis as the characterization technique for the enantioselective transformations. Our study has expanded the application of the CEA reaction coupled with LC/MS analysis to mixtures. Utilizing LC/MS analysis, the CEA reaction offers a sensitive and simple method for stereochemistry determination. Additionally, the application of the CEA reaction is cost/time-effective since only small quantities of substrates and a short reaction time are required for characterizing the absolute configuration of secondary alcohols in natural products compared to other conventional methods.

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“…Since most ginsenosides, such as Rb2, exert a poor absorption rate and rapid tissue distribution, an optimal biocarrier should be developed for a long-lasting effect of ginsenosides on the human body [ 186 , 187 ]. Promisingly, there are growing efforts to build biocompatible carrier packaging ginsenosides [ 188 , 189 ]; for instance, an Rg3-loaded hydrogel, made with mPEG-b-PLGA polymers for increased delivery efficiency, was developed to target perianal ulcers in a rat model [ 190 ].…”

Section: Discussionmentioning

confidence: 99%

Ginsenosides from Panax ginseng as Key Modulators of NF-κB Signaling Are Powerful Anti-Inflammatory and Anticancer Agents

Jang

Hwang

Cho

2023

IJMS

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Nuclear factor kappa B (NF-κB) signaling pathways progress inflammation and immune cell differentiation in the host immune response; however, the uncontrollable stimulation of NF-κB signaling is responsible for several inflammatory illnesses regardless of whether the conditions are acute or chronic. Innate immune cells, such as macrophages, microglia, and Kupffer cells, secrete pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β, via the activation of NF-κB subunits, which may lead to the damage of normal cells, including neurons, cardiomyocytes, hepatocytes, and alveolar cells. This results in the occurrence of neurodegenerative disorders, cardiac infarction, or liver injury, which may eventually lead to systemic inflammation or cancer. Recently, ginsenosides from Panax ginseng, a historical herbal plant used in East Asia, have been used as possible options for curing inflammatory diseases. All of the ginsenosides tested target different steps of the NF-κB signaling pathway, ameliorating the symptoms of severe illnesses. Moreover, ginsenosides inhibit the NF-κB-mediated activation of cancer metastasis and immune resistance, significantly attenuating the expression of MMPs, Snail, Slug, TWIST1, and PD-L1. This review introduces current studies on the therapeutic efficacy of ginsenosides in alleviating NF-κB responses and emphasizes the critical role of ginsenosides in severe inflammatory diseases as well as cancers.

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Evaluation of 20(S)-ginsenoside Rg3 loaded hydrogel for the treatment of perianal ulcer in a rat model

Cited by 8 publications

References 41 publications

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

N-Hydroxy-Phe-Phe, a new dipeptide, and cytotoxic macrocyclic trichothecenes from the lethal toxic mushroom Podostroma cornu-damae

Determination of the Absolute Configuration of Secondary Alcohols in a Compound Mixture via the Application of Competing Enantioselective Acylation Coupled with LC/MS Analysis

Ginsenosides from Panax ginseng as Key Modulators of NF-κB Signaling Are Powerful Anti-Inflammatory and Anticancer Agents

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