Evaluation of 13-week subchronic toxicity of Platycodon grandiflorus (Jacq.) A.DC. root extract in rats

Cha, Seung-Beom; Li, Yinghua; Bae, Jong‐Sup; Song, Si-Whan; Lee, In-Chul; Kim, Jong-Choon

doi:10.1016/j.jep.2020.113621

Cited by 10 publications

(10 citation statements)

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“…Platycodin D content of the Platycodi radix water extract was measured using high-performance liquid chromatography (HPLC) systems with evaporative light scattering detection. A 100 mg/mL Platycodi radix water extract was used for experiments with the above conditions which was performed three times, and the Platycodi radix water extract showed an average content of 0.370% and the relative standard deviation (RSD) was superior to less than 5% 3 . The Platycodi radix water extract powder was prepared by suspension in sterile water (Daihan Pharm.…”

Section: Preparation Of Platycodi Radix Water Extractmentioning

confidence: 99%

“…The animals were administered 500, 1000, and 3000 mg/kg b.w./day of Platycodi radix water extract for 13 weeks by oral gavage. The dose selection was based on the results of a preliminary 2-week repeated oral toxicity study (data not shown) 3 . This study also aimed to investigate the recovery potential during a 4-week recovery period.…”

Section: Experimental Animals and Treatmentmentioning

confidence: 99%

“…The test article induced adaptive non-adverse changes, centrilobular hepatocellular hypertrophy in the liver, and diffuse follicular cell hypertrophy in the thyroid gland 3 ; however, they were not analyzed in this study.…”

Section: Histopathological Examinationmentioning

confidence: 99%

“…A. DC., which is widely grown in northern Asia, China, Korea, Japan, and East Siberia 2,3 and it is typically used as a component for preparing salads, cold soups, and sauces in Asia 4 . Platycodi radix has been generally used in traditional herbal medicine for bronchitis, asthma, pulmonary tuberculosis, hypertension, hyperlipidemia, and diabetes [5][6][7] .The local irritating effect is presumably potentiated by the increased duration of exposure associated with intragastric storage and increased contact, when a test article precipitates in the gastric compartment.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, we determined the proliferation tendency of squamous cell hyperplasia of the limiting ridge in the stomach induced by the Platycodi radix water extract in SD rats. This article focuses on the reversibility assessment using immunohistochemistry endpoints (Caspase-3 and Ki-67) for the Platycodi radix water extract-induced gastric finding that was published in the Journal of Ethnopharmacology by Cha et al 3 .…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Caspase-3 and Ki-67 expression in squamous cell hyperplasia of the stomach induced by <i>Platycodi radix</i> water extract in Sprague–Dawley rats

Li¹,

Cha²,

Park

et al. 2022

J Toxicol Pathol

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Platycodi radix is widely used in traditional herbal medicine for the treatment of bronchitis, asthma, pulmonary tuberculosis, hypertension, hyperlipidemia, and diabetes. This study aimed to investigate cell proliferation (Ki-67) and apoptosis (Caspase-3) potential in squamous cell hyperplasia of the stomach induced by a Platycodi radix water extract in a subchronic toxicity study. One hundred formalin-fixed, paraffin-embedded stomach tissues of rats treated with Platycodi radix at doses of 0, 500, 1,000, and 3,000 mg/kg body weight/day were used for the analysis. They were conventionally stained using hematoxylin and eosin (H&E) and immunohistochemically (IHC) stained using caspase-3 and Ki-67 antibodies. The incidence of squamous cell hyperplasia was significantly increased in the 3000 mg/kg b.w./day treatment group in both sexes (p < 0.01). However, the hyperplastic change was completely repaired after 4 weeks of recovery period. Ki-67 expression was similar in all groups, with no statistically significant differences among the groups. Caspase-3 expression was significantly increased in both sexes in the 3000 mg/kg b.w./day treatment group (p < 0.01), compared with the vehicle control groups, and then reduced to normal levels in the recovery groups in both sexes. In conclusion, this study showed that squamous cell hyperplasia induced by the Platycodi radix water extract in the limiting ridge of the stomach is not considered to be abnormal proliferative change; as a result, squamous cell hyperplasia is considered to be a non-adverse effect when induced by the oral administration of the Platycodi radix water extract once daily for 13 weeks in rats.

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Section: Preparation Of Platycodi Radix Water Extractmentioning

confidence: 99%

Section: Experimental Animals and Treatmentmentioning

confidence: 99%

Section: Histopathological Examinationmentioning

confidence: 99%