Évaluation de la toxicité deBacillus thuringiensis surSpodoptera frugiperda

Hernandez, Julie

doi:10.1007/bf02372651

Cited by 19 publications

(5 citation statements)

References 13 publications

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“…Polanczyk et al (1999) evaluated in vivo activities of Bt strains on S. frugiperda, and observed that suspensions of Bt aizawai HD 68 and Bt thuringiensis 4412, containing 3x 10 8 cells/ ml, induced effective mortality. Similar results were obtained by Hernandez (1988). Ramanujam et al (2020) with field trials observed efficacy of three sprays of M. anisopliae ICAR-NBAIR Ma-35 and B. bassiana ICAR-NBAIR Bb-45.…”

Section: Resultssupporting

confidence: 85%

Biorational management of spodoptera frugiperda (J E Smith) on maize

Wayal¹,

Unidirwadwade

Jawanjal

et al. 2022

ije

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The present study on the biorational management of Spodoptera frugiperda (J E Smith) on maize was conducted in the farm of Department of Agricultural Entomology, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola during kharif 2019-20. The experiment was laid out in randomized block design with eight treatments replicated thrice. The treatments included- Nomuraea rileyi 1x108 cfu/ g @ 30 g/ 10 l, Metarhizium anisopliae 1x108 cfu/ g @ 50 g/ 10 l, Beauveria bassiana 1x109 cfu/ g @ 40 g/ 10 l, NSKE 5%, Bt 85% @ 20 g/ 10 l, SLNPV 1x109 POB/ ml (500 LE), azadirachtin 1500 ppm @ 50 ml/ 10 l, and control (untreated). Four sprays were applied at 12 days interval. The results revealed that with fourth spray, Bt 85% @ 20 g/ 10 l was the most effective.

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Section: Resultssupporting

confidence: 85%

Biorational management of spodoptera frugiperda (J E Smith) on maize

Wayal¹,

Unidirwadwade

Jawanjal

et al. 2022

ije

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“…The results obtained in this study appeared that transcojugant Tr 4 is higher pathogenic causing 100% toxicity index than other bioinsecticides used in this study .The same trend was obtained by Hernadez ( 1988 ) on subspecies Bt aizawai , B. thuringiensis and Bt Kurstaki, who observing mortality of 80% , 100 % and 70 % respectively , using 3 x10 7 cells / ml . The virulence assays showed that transconjugant Tr 4 was the most active recombinant isolate with an LC 50 of 1.93x10 5 ppm followed by Tr 6 , Tr 7 Tr 1 and Tr 5 with an LC 50 of 2x 10 5 , 2x10 5 , 2.3 x10 5 , 2.3 x10 5 ppm d c a b a b…”

Section: Conjugation and Hydrolysis Of Colloidal Chitinsupporting

confidence: 82%

A Method to Determine Lethal and Sublethal Concentrations of Recombinant Bioinsecticides Derived from Bacillus thuringiensis Against Larvae of Spodoptera litura

Adl

Zaied

Kash

et al. 2016

Journal of Agricultural Chemistry and Biotechnology

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The increased awareness of environmental pollution have led to the growing interests of the application of biological agents to control insects such as lepidopteran family .Thus , this study aimed to produce genetically improved bioinsecticide agents to be used to control Spodoptera litura which would be useful in reducing environmental pollution ,preventing resistance to bioinsecticides and for safe food production .Four Bacillus thuringensis strains were used as recipients ' while ' Seratia marcescens was used as a doner strain in the conjugation to induce new recombinants in Bacillus thuringiensis over expressed chitinase.Four matings were conducted in this study between Serratia marcescence and the four Bacillus thuringiensis strains which having opposite genetic markers.Two efficient transconjugants based on chitin hydrolysis on agar medium were selected from each conjugation to be evaluated for toxicites against the larvae of cotton leafworm in relation to their parents .The efficient LC 50 was appeared by Tr 4 ( 1.93 x10 5 ppm ) followed by Tr 6 and Tr 7 ( 2x10 5 ppm). However , more than 995% toxicity index was recorded by Tr 4 ,Tr 6 and Tr 7 . Higher toxicity index observed by these transconjugants may be the related to the overexpression of chitinase genes.Therefore , the lethal concentration values obtained was necessary to kill 50% of larval population from Spodoptera litura in a certain period .The virulence of Bt strains showed that a lower concentration from Tr 4 was required to kill 100% of larval population .It must be pointed out that higher toxicity index obtained by transconjugants reflected the interaction between crystal genes from Bt and chitinase genes from Seratia marcescens which enhanced the possibility of larval mortality as a genetic helpful tool in integrated pest management in cotton fields.

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“…Some time ago, the efficacy of this microorganism against S. frugiperda was considered questionable, but more recently the increment in researches on the use of Bt against this lepidopterous brought some interesting results (4,10). The objective of this work was to report the selection of Bt strains with potential to control the fall armyworm.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Bacillus thuringiensis strains against Spodoptera frugiperda (lepidoptera: noctuidae)

Polanczyk¹,

Silva²,

Fiúza³

2000

Braz. J. Microbiol.

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The fall armyworm (Spodoptera frugiperda) is one of the most important pests of maize, causing up to 20% production losses when defoliation occurs near to flowering, or even complete destruction of plants. Among the alternatives to control this pest, the use of Bacillus thuringiensis (Bt) has gained attention due to its efficiency and low impact on natural enemies. Strains Bt dendrolimus HD 37, Bt aizawai HD 68, Bt kurstaki HD 73, Bt darmstadiensis HD 146, and Bt thuringiensis 4412 were tested against second instar larvae in in vivo assays. Suspensions of Bt aizawai HD 68 and Bt thuringiensis 4412, containing 3 x 10(8) cells/ml, induced mortality of 100% and 80.4%, respectively. To test virulence, cell concentrations of 8 x 10(5) to 3 x 10(8) cells/ml of strains Bt aizawai HD 68 and Bt thuringiensis 4412 were applied on the second instar larvae: LC50 were 6.7 x 10(6) and 8.6 x 10(6) cells/ml, respectively

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Évaluation de la toxicité deBacillus thuringiensis surSpodoptera frugiperda

Cited by 19 publications

References 13 publications

Biorational management of spodoptera frugiperda (J E Smith) on maize

Biorational management of spodoptera frugiperda (J E Smith) on maize

A Method to Determine Lethal and Sublethal Concentrations of Recombinant Bioinsecticides Derived from Bacillus thuringiensis Against Larvae of Spodoptera litura

Effectiveness of Bacillus thuringiensis strains against Spodoptera frugiperda (lepidoptera: noctuidae)

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