Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings

Zhang, Hongmei; Xu, Wenjing; Chen, Huatao; Chen, Jingbin; Chen, Xin; Yang, Shouping

doi:10.1007/s11105-020-01194-2

Cited by 5 publications

(5 citation statements)

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“…Based on our previous studies, six major QTLs associated with salt tolerance were detected in the region between VUIn584 ~ VUIn724, between VUIn282 ~ VUIn815, and between VUIn675 ~ VUIn578 on chromosomes 9, 11, and 8 [ 24 ]. In the present study, nine DEGs in the region of VUIn282 ~ VUIn815 on Chr.11 were found, eight of which were differentially expressed only in Suzi 41 under salt treatment (Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…The seeds for this study contained salt-tolerant Suzi 41 and salt-sensitive Sujiang 1419 [ 24 ], and 84 other yardlong bean accessions, among which 77 were collected from the National Infrastructure for Vegetable Crop Germplasm Resources (NIVCGR), and 7 from Jiangsu Academy of Agricultural Sciences (JAAS) (Supplementary Table S 13 ).…”

Section: Methodsmentioning

confidence: 99%

“…Two yardlong bean genotypes: salt-tolerant Suzi 41 and salt-sensitive Sujiang 1419 [ 24 ] were employed to examine differences in the expression of genes involved in a tolerant response to high salinity. Ten seeds of Suzi 41 and Sujiang 1419 were sown in cups (9.5 × 16 cm) filled with vermiculite, with three replicates per genotype.…”

Section: Methodsmentioning

confidence: 99%

“…Tan et al (2016) identified several genes that were differentially expressed genes (DEGs) between cold-tolerant and -sensitive yardlong bean cultivars, while Pan et al (2019) found 216 and 127 salt stress-associated DEGs in roots and leaves, respectively, six of which were linked to 17 salt tolerance-associated SNP markers [ 22 , 23 ]. More recently, other QTLs associated with salt tolerance in yardlong bean were mapped using a population generated by crossing Suzi 41 (salt tolerant) × Sujiang 1419 (salt sensitive) [ 24 ]. Completion of the yardlong bean genome and the relatively low cost of re-sequencing have enabled further development of SNP/InDel markers in sesquipedalis for use in breeding and genetic analysis, as has been widely reported in common bean and mungbean [ 25 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of salt tolerance-associated genes and diversity analysis using indel markers in yardlong bean (Vigna unguiculata ssp. sesquipedialis)

Zhang

Chen

et al. 2021

BMC Genom Data

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Background High salinity is a devastating abiotic stresses for crops. To understand the molecular basis of salinity stress in yardlong bean (Vigna unguiculata ssp. sesquipedalis), and to develop robust markers for improving this trait in germplasm, whole transcriptome RNA sequencing (RNA-seq) was conducted to compare the salt-tolerant variety Suzi 41 and salt-sensitive variety Sujiang 1419 under normal and salt stress conditions. Results Compared with controls, 417 differentially expressed genes (DEGs) were identified under exposure to high salinity, including 42 up- and 11 down-regulated DEGs in salt-tolerant Suzi 41 and 186 up- and 197 down-regulated genes in salt-sensitive Sujiang 1419, validated by qRT-PCR. DEGs were enriched in “Glycolysis/Gluconeogenesis” (ko00010), “Cutin, suberine and wax biosynthesis” (ko00073), and “phenylpropanoid biosynthesis” (ko00940) in Sujiang 1419, although “cysteine/methionine metabolism” (ko00270) was the only pathway significantly enriched in salt-tolerant Suzi 41. Notably, AP2/ERF, LR48, WRKY, and bHLH family transcription factors (TFs) were up-regulated under high salt conditions. Genetic diversity analysis of 84 yardlong bean accessions using 26 InDel markers developed here could distinguish salt-tolerant and salt-sensitive varieties. Conclusions These findings show a limited set of DEGs, primarily TFs, respond to salinity stress in V. unguiculata, and that these InDels associated with salt-inducible loci are reliable for diversity analysis.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic analysis of salt tolerance-associated genes and diversity analysis using indel markers in yardlong bean (Vigna unguiculata ssp. sesquipedialis)

Zhang

Chen

et al. 2021

BMC Genom Data

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“…ssp. unguiculata (cowpea), are widely grown and consumed in various regions, including in Africa, Asia, North America, and Europe [1][2][3]. These subspecies are preferred for their ability to withstand heat and drought, making them a favorable choice for cultivation in the face of changing environments and climate conditions [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Multivariate Analysis of Biochemical Properties Reveals Diversity among Yardlong Beans of Different Origins

Choi,

Yoon,

Shin

et al. 2024

Antioxidants

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This study analyzed the nutrient levels, secondary metabolite contents, and antioxidant activities of 35 yardlong bean accessions from China, Korea, Myanmar, and Thailand, along with their key agronomic traits. Significant variations were found in all the parameters analyzed (p < 0.05). The crude fiber (CFC), dietary fiber (DFC), total protein, and total fat contents varied from 4.10 to 6.51%, 16.71 to 23.49%, 22.45 to 28.11%, and 0.59 to 2.00%, respectively. HPLC analysis showed more than a 10-fold difference in vitamin C level (0.23 to 3.04 mg/g), whereas GC-FID analysis revealed the dominance of palmitic acid and linoleic acid. All accessions had high levels of total unsaturated fatty acids, which could help in preventing cardiovascular disease. Furthermore, total phenolic, tannin, and saponin contents ranged between 3.78 and 9.13 mg GAE/g, 31.20 and 778.34 mg CE/g, and 25.79 and 82.55 mg DE/g, respectively. Antioxidant activities like DPPH• scavenging, ABTS•+ scavenging, and reducing power (RP) ranged between 1.63 and 9.95 mg AAE/g, 6.51 and 21.21 mg TE/g, and 2.02, and 15.58 mg AAE/g, respectively. Days to flowering, total fat, palmitic acid, oleic acid, and TPC were significantly influenced by origin and genotype differences, while seeds per pod, one-hundred seeds weight, CFC, DFC, vitamin C, RP, and TSC were not affected by these factors. Multivariate analysis categorized the accessions into four clusters showing significant variations in most of the analyzed parameters. Correlation analysis also revealed significant relationships between several noteworthy parameters. Overall, this comprehensive analysis of biochemical factors revealed diversity among the different yardlong bean varieties. These findings could have practical applications in industries, breeding programs, and conservation efforts.

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Genetic Improvement of Yardlong Bean (Vigna unguiculata (L.) Walp. ssp. sesquipedalis (L.) Verdc.)

Saidaiah

Thuraga

Pandravada

et al. 2021

Advances in Plant Breeding Strategies: Vegetable Crops

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Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings

Cited by 5 publications

References 60 publications

Transcriptomic analysis of salt tolerance-associated genes and diversity analysis using indel markers in yardlong bean (Vigna unguiculata ssp. sesquipedialis)

Transcriptomic analysis of salt tolerance-associated genes and diversity analysis using indel markers in yardlong bean (Vigna unguiculata ssp. sesquipedialis)

Multivariate Analysis of Biochemical Properties Reveals Diversity among Yardlong Beans of Different Origins

Genetic Improvement of Yardlong Bean (Vigna unguiculata (L.) Walp. ssp. sesquipedalis (L.) Verdc.)

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