Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer – HemosIL reagents

Miloš, Marija; Herak, Désirée Coen; Kuric, L.; Horvat, Ivana; Zadro, Renata

doi:10.1111/j.1751-553x.2007.00999.x

Cited by 38 publications

(28 citation statements)

References 17 publications

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“…IgG anticardiolipin has a higher predictive value than IgM or IgA of a thrombosis. Beta-2 glycoprotein I has been identified as one of the major antigenic determinants of antiphospholipid antibodies 11, 12, 13, 14…”

Section: Introductionmentioning

confidence: 99%

Diagnostic testing and interpretation of tests for autoimmunity

Castro

Gourley

2010

Journal of Allergy and Clinical Immunology

135

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Laboratory testing is of great value when evaluating a patient with a suspected autoimmune disease. The results can confirm a diagnosis, estimate disease severity, aid in assessing prognosis and are useful to follow disease activity. Components of the laboratory exam include complete blood count with differential, comprehensive metabolic panel, inflammatory markers, autoantibodies, and flow cytometry. This chapter discusses these components and includes a discussion about organ-specific immunologic diseases where immunological laboratory testing is employed. Comprehensive laboratory evaluation of a suspected autoimmune illness in conjunction with a thorough clinical evaluation provides a better understanding of a patient's immunologic disease.

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Section: Introductionmentioning

confidence: 99%

Diagnostic testing and interpretation of tests for autoimmunity

Castro

Gourley

2010

Journal of Allergy and Clinical Immunology

135

View full text Add to dashboard Cite

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“…The instrument was calibrated against appropriate proprietary reference standard material and verified with the use of proprietary quality controls. A reported performance evaluation of the within-run precision of the automated coagulation analyzer ACL TOP in normal plasma showed coefficients of variation ranging from 1.6% for aPTT to 3.5% for FIB [12]. Our evaluation of the within-run precision by internal quality control on ACL TOP showed low coefficients of variation.…”

Section: Laboratory Testingmentioning

confidence: 57%

Sodium citrate vacuum tubes validation

Lima-Oliveira

Lippi

Salvagno

et al. 2013

Blood Coagulation &Amp; Fibrinolysis

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Sometimes in-vitro diagnostic devices (e.g. blood collection tubes) are not validated before use or when the producer's brand is changed. The aim of this study was to validate five brands of sodium citrate vacuum tubes. Blood specimens from 50 volunteers were collected in five different tube brands (I: Venosafe, II: VACUETTE, III: BD Vacutainer, IV: LABOR IMPORT and V: S-Monovette). Routine coagulation tests [activated partial thromboplastin time (aPTT), prothrombin time (PT), and fibrinogen (FIB)] were performed on ACL TOP instrument using HemosIL reagents. The significance of the differences between samples was assessed by paired Student's t-test, set at P < 0.005. Significant differences were observed for: PT when comparing I vs. II, I vs. III, I vs. V, II vs. III, II vs. IV, II vs. V, III vs. IV, III vs. V and IV vs. V; aPTT when comparing I vs. II, I vs. III, I vs. IV, II vs. IV, III vs. IV and IV vs. V. No differences were observed among brands for FIB determination. We suggest that every laboratory management should both standardize the procedures and frequently evaluate the quality of in-vitro diagnostic devices.

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“…Prothrombin time, APTT, and fibrinogen were assayed using the same fully automated coagulation analyzer ACL TOP 700 (Instrumentation Laboratory Bedford, MA, USA), with RecombiPlasTin (Instrumentation Laboratory), SynthASil (Instrumentation Laboratory) and Fibrinogen‐CXL (Instrumentation Laboratory), respectively. The analytical performance of these tests has been earlier described elsewhere . Immediately after collection, all blood tubes were centrifuged at 1300 × g for 15 minutes at room temperature, in accordance with the current recommendations of the Clinical Laboratory Standards Institute (CLSI) .…”

Section: Methodsmentioning

confidence: 99%

Impact of low volume citrate tubes on results of first‐line hemostasis testing

Salvagno

Demonte

Poli

et al. 2019

Int J Lab Hematology

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Introduction Pediatric tubes are increasingly used for drawing blood for hemostasis testing. This study has investigated the potential impact of low volume citrate tubes on results of first‐line hemostasis testing. Methods The study population comprised 34 patients on warfarin therapy and 17 ostensibly healthy volunteers. Blood was collected into five different evacuated blood tubes from each subject. On right arm, blood was drawn directly into two standard evacuated blood tubes (3‐mL Vacuette and 2‐mL Vacutest) and one evacuated low volume blood tube (1‐mL Vacuette) by straight needle venipuncture. On left arm, blood was drawn using a 5‐mL syringe and then transferred within two nonevacuated microtubes (0.5 mL MiniCollect and 0.5 mL Micro Test). Prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen were assayed on ACL TOP 700. Results Spearman's correlation of PT, APTT, and fibrinogen values obtained using different tubes was always satisfactory (ie, ≥0.93). A statistically significant bias was frequently found by comparing values obtained in different tubes. Nevertheless, the minimum quality specifications for bias were exceeded only by comparing data of Vacuette 1 mL with those of all other blood tubes for PT, by comparing data of Micro Test 0.5 mL with those of all other blood tubes for APTT, and by comparing data of Micro Test 0.5‐mL blood tubes with those of Vacuette 3 mL and Vacuette 1. Conclusion First‐line hemostasis testing using low volume citrate tubes may display differences sometimes exceeding the minimum quality specifications.

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Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer – HemosIL reagents

Cited by 38 publications

References 17 publications

Diagnostic testing and interpretation of tests for autoimmunity

Diagnostic testing and interpretation of tests for autoimmunity

Sodium citrate vacuum tubes validation

Impact of low volume citrate tubes on results of first‐line hemostasis testing

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