Evaluation and Optimization of Poly-d-Lysine as a Non-Natural Cationic Polypeptide for Gene Transfer in Neuroblastoma Cells

Sánchez-Martos, Miguel; Martı́nez, Gema; Bernabeu-Zornoza, Adela; Humphreys, Lawrence; Fernández, Eduardo

doi:10.3390/nano11071756

Cited by 2 publications

(2 citation statements)

References 57 publications

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“…Finally, it was reported that poly-D-lysine (DPL) conjugates showed higher transfection capacity than PLL ones, as they were more stable for both lysosomal and peptidase degradation [70].…”

Section: Polymeric Carriers 221 Poly(l-lysine) (Pll)mentioning

confidence: 99%

Cationic Materials for Gene Therapy: A Look Back to the Birth and Development of 2,2-Bis-(hydroxymethyl)Propanoic Acid-Based Dendrimer Scaffolds

Alfei

2023

IJMS

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Gene therapy is extensively studied as a realistic and promising therapeutic approach for treating inherited and acquired diseases by repairing defective genes through introducing (transfection) the “healthy” genetic material in the diseased cells. To succeed, the proper DNA or RNA fragments need efficient vectors, and viruses are endowed with excellent transfection efficiency and have been extensively exploited. Due to several drawbacks related to their use, nonviral cationic materials, including lipidic, polymeric, and dendrimer vectors capable of electrostatically interacting with anionic phosphate groups of genetic material, represent appealing alternative options to viral carriers. Particularly, dendrimers are highly branched, nanosized synthetic polymers characterized by a globular structure, low polydispersity index, presence of internal cavities, and a large number of peripheral functional groups exploitable to bind cationic moieties. Dendrimers are successful in several biomedical applications and are currently extensively studied for nonviral gene delivery. Among dendrimers, those derived by 2,2-bis(hydroxymethyl)propanoic acid (b-HMPA), having, unlike PAMAMs, a neutral polyester-based scaffold, could be particularly good-looking due to their degradability in vivo. Here, an overview of gene therapy, its objectives and challenges, and the main cationic materials studied for transporting and delivering genetic materials have been reported. Subsequently, due to their high potential for application in vivo, we have focused on the biodegradable dendrimer scaffolds, telling the history of the birth and development of b-HMPA-derived dendrimers. Finally, thanks to a personal experience in the synthesis of b-HMPA-based dendrimers, our contribution to this field has been described. In particular, we have enriched this work by reporting about the b-HMPA-based derivatives peripherally functionalized with amino acids prepared by us in recent years, thus rendering this paper original and different from the existing reviews.

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“…Finally, it was reported that poly-D-lysine (DPL) conjugates showed higher transfection capacity than PLL ones, as they were more stable for both lysosomal and peptidase degradation [70].…”

Section: Polymeric Carriers 221 Poly(l-lysine) (Pll)mentioning

confidence: 99%

Cationic Materials for Gene Therapy: A Look Back to the Birth and Development of 2,2-Bis-(hydroxymethyl)Propanoic Acid-Based Dendrimer Scaffolds

Alfei

2023

IJMS

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“…Under the treatment of MXD3 ASO-SPIO NP complex, MXD3 expression was down-regulated and apoptosis was significantly induced in NB cells. Miguel et al identified D-lysine polymer as an effective gene delivery vehicle and could be used as a synthetic cell-penetrating peptide for gene therapy in the SH-SY5Y cell ( Sanchez-Martos et al, 2021 ). Another study found that rabies virus glycoprotein peptide-modified poly D, L-lactide-co-glycolide NPs could specifically target NB in vitro and in vivo and significantly inhibited tumor growth in a mouse model ( Lee et al, 2016b ).…”

Section: Nanotechnology Enhances Therapeutic Treatment For Neuroblastomamentioning

confidence: 99%

Nanotechnology-Based Diagnostic and Therapeutic Strategies for Neuroblastoma

et al. 2022

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Neuroblastoma (NB), as the most common extracranial solid tumor in childhood, is one of the critical culprits affecting children’s health. Given the heterogeneity and invisibility of NB tumors, the existing diagnostic and therapeutic approaches are inadequate and ineffective in early screening and prognostic improvement. With the rapid innovation and development of nanotechnology, nanomedicines have attracted widespread attention in the field of oncology research for their excellent physiological and chemical properties. In this review, we first explored the current common obstacles in the diagnosis and treatment of NB. Then we comprehensively summarized the advancements in nanotechnology-based multimodal synergistic diagnosis and treatment of NB and elucidate the underlying mechanisms. In addition, a discussion of the pending challenges in biocompatibility and toxicity of nanomedicine was conducted. Finally, we described the development and application status of nanomaterials against some of the recognized targets in the field of NB research, and pointed out prospects for nanomedicine-based precision diagnosis and therapy of NB.

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Evaluation and Optimization of Poly-d-Lysine as a Non-Natural Cationic Polypeptide for Gene Transfer in Neuroblastoma Cells

Cited by 2 publications

References 57 publications

Cationic Materials for Gene Therapy: A Look Back to the Birth and Development of 2,2-Bis-(hydroxymethyl)Propanoic Acid-Based Dendrimer Scaffolds

Cationic Materials for Gene Therapy: A Look Back to the Birth and Development of 2,2-Bis-(hydroxymethyl)Propanoic Acid-Based Dendrimer Scaffolds

Nanotechnology-Based Diagnostic and Therapeutic Strategies for Neuroblastoma

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