Evaluation and genetic analysis of grapevine black rot resistances

Hausmann, Ludger; Rex, Friederike; Töpfer, Reinhard

doi:10.17660/actahortic.2017.1188.37

Cited by 6 publications

(12 citation statements)

References 15 publications

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“…Moreover, the developed highly dense map allowed for narrowing down the size of the QTL to 0.7 Mb. The same region has been identified also by Hausmann et al [ 40 ] in a breeding population with a complex inter-specific background ( V. aestivalis var. lincecumii × V. cinerea var.…”

Section: Discussionsupporting

confidence: 65%

“…The SNP dataset was integrated with 192 SSR markers of the M × T map published by Vezzulli et al [ 88 ]. Finally, three SSR markers that were previously associated with the Rgb 1 locus [ 40 ] were amplified in triplex with KAPA2G Fast Multiplex Mix (Sigma-Aldrich/Merck, USA) and included in this study, namely GF14-42 [ 21 ], GF14-04 [ 94 ] and UDV-095 [ 41 ]. The amplification followed a touchdown protocol (60 to 55 °C, −0.5 °C × 10 cycles).…”

Section: Methodsmentioning

confidence: 99%

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Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’

Bettinelli

Nicolini

Costantini

et al. 2023

IJMS

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Black rot (BR), caused by Guignardia bidwellii, is an emergent fungal disease threatening viticulture and affecting several mildew-tolerant varieties. However, its genetic bases are not fully dissected yet. For this purpose, a segregating population derived from the cross ‘Merzling’ (hybrid, resistant) × ‘Teroldego’ (V. vinifera, susceptible) was evaluated for BR resistance at the shoot and bunch level. The progeny was genotyped with the GrapeReSeq Illumina 20K SNPchip, and 7175 SNPs were combined with 194 SSRs to generate a high-density linkage map of 1677 cM. The QTL analysis based on shoot trials confirmed the previously identified Resistance to Guignardia bidwellii (Rgb)1 locus on chromosome 14, which explained up to 29.2% of the phenotypic variance, reducing the genomic interval from 2.4 to 0.7 Mb. Upstream of Rgb1, this study revealed a new QTL explaining up to 79.9% of the variance for bunch resistance, designated Rgb3. The physical region encompassing the two QTLs does not underlie annotated resistance (R)-genes. The Rgb1 locus resulted enriched in genes belonging to phloem dynamics and mitochondrial proton transfer, while Rgb3 presented a cluster of pathogenesis-related Germin-like protein genes, promoters of the programmed cell death. These outcomes suggest a strong involvement of mitochondrial oxidative burst and phloem occlusion in BR resistance mechanisms and provide new molecular tools for grapevine marker-assisted breeding.

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Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’

Bettinelli

Nicolini

Costantini

et al. 2023

IJMS

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“…This study revealed the presence of seven R -loci distributed over the genome. The major QTL on chromosome 14 in this mapping population was tightly linked to the SSR markers GF14-04 and UDV-095, both are located in the vicinity of the above mentioned markers CS25b and GF14-42 (Hausmann et al, 2017) ( Table 1 ). All these data suggest that the major R -locus on chromosome 14, identified in three different biparental population, plays a leading role in conferring BR resistance.…”

Section: Grapevine-ascomycete Interactionmentioning

confidence: 91%

“…In contrast to PM, for which several R -loci have already been discovered in various grapevine cultivars, very little is known about the genetic loci involved in BR resistance ( Table 1 ). It has been observed almost a century ago that North American and French hybrids are more resistant to G. bidwellii infection, while V. vinifera is extremely susceptible (Demaree et al, 1937; Barrett, 1955; Hausmann et al, 2017). The American origin of this ascomycete explains the occurrence of genetic resistance in American Vitis species, due to their coevolution (Ramsdell and Milholland, 1988; Hausmann et al, 2017).…”

Section: Grapevine-ascomycete Interactionmentioning

confidence: 99%

“…It has been observed almost a century ago that North American and French hybrids are more resistant to G. bidwellii infection, while V. vinifera is extremely susceptible (Demaree et al, 1937; Barrett, 1955; Hausmann et al, 2017). The American origin of this ascomycete explains the occurrence of genetic resistance in American Vitis species, due to their coevolution (Ramsdell and Milholland, 1988; Hausmann et al, 2017). Hints about the genetic segregation pattern of BR resistance were suggested in a ‘Blue Lake’ progeny by Mortensen (1977), but the first QTL analysis was performed much later by Dalbó et al (2000).…”

Section: Grapevine-ascomycete Interactionmentioning

confidence: 99%

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Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

et al. 2019

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The reduction of pesticide usage is a current imperative and the implementation of sustainable viticulture is an urgent necessity. A potential solution, which is being increasingly adopted, is offered by the use of grapevine cultivars resistant to its main pathogenic threats. This, however, has contributed to changes in defense strategies resulting in the occurrence of secondary diseases, which were previously controlled. Concomitantly, the ongoing climate crisis is contributing to destabilizing the increasingly dynamic viticultural context. In this review, we explore the available knowledge on three Ascomycetes which are considered emergent and causal agents of powdery mildew, black rot and anthracnose. We also aim to provide a survey on methods for phenotyping disease symptoms in fields, greenhouse and lab conditions, and for disease control underlying the insurgence of pathogen resistance to fungicide. Thus, we discuss fungal genetic variability, highlighting the usage and development of molecular markers and barcoding, coupled with genome sequencing. Moreover, we extensively report on the current knowledge available on grapevine-ascomycete interactions, as well as the mechanisms developed by the host to counteract the attack. Indeed, to better understand these resistance mechanisms, it is relevant to identify pathogen effectors which are involved in the infection process and how grapevine resistance genes function and impact the downstream cascade. Dealing with such a wealth of information on both pathogens and the host, the horizon is now represented by multidisciplinary approaches, combining traditional and innovative methods of cultivation. This will support the translation from theory to practice, in an attempt to understand biology very deeply and manage the spread of these Ascomycetes.

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