Evaluation and comparison of molecular and conventional diagnostic modalities for detecting pulmonary tuberculosis in bronchoalveolar lavage fluid

Bhatia, Disha; Bhatia, Nirmaljit Kaur; Deepak, Desh; Sharma, Bharti; Shulania, Anuradha; Duggal, Nandini

doi:10.1016/j.ijmmb.2020.10.003

Cited by 2 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Xpert MTB/RIF had the highest positive rate, followed by TB‐DNA, and Bactec MGIT960 had the lowest, which was the same order in which we retained specimens. However, most studies suggest that the diagnostic yield is similar among these three 26 . The possibility of this result is that the lesions are relatively small, and the concentration of Mycobacterium tuberculosis in the lavage fluid decreases in turn as the lavage is performed, resulting in differences in the diagnostic yield of the three.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of ultrasound‐guided transbronchial lung biopsy in peripheral tuberculous pulmonary lesions

Zou

Zhu

et al. 2022

Diagnostic Cytopathology

View full text Add to dashboard Cite

Background: To investigate the diagnostic value of bronchial ultrasound-guided transbronchial lung biopsy (EBUS-TBLB) in tuberculous peripheral pulmonary lesions.Methods: A total of 770 patients who completed CT imaging and ultrasound bronchoscopy were retrospectively analyzed. All patients underwent biopsy sampling as well as alveolar lavage under the guidance of ultrasound. Pathological analysis and molecular biological detection of pulmonary tuberculosis were performed in both pathological tissues and bronchoalveolar lavage fluid (BALF), and the diagnostic positive rate and diagnostic sensitivity were statistically analyzed.Results: Of the 44 patients who were found to have lesions by EBUS-TBLB, 26 patients were able to achieve a definite diagnosis of PPLs, with an overall diagnostic yield of 59.1%. Of the 33 patients with all diagnosed benign lesions, 22 were diagnosed with active pulmonary tuberculosis with the diagnostic yield of 66.7%. Among above 22 cases, the overall positive rate of BALF diagnosis was as high as 95.6%, and the highest diagnostic rate of a single test was BALF XpertMTB/RIF, 59.1%. Compared with pathological tissues, the diagnostic positive rate of BALF as a diagnostic specimen was higher (p < .05). In addition, the diagnostic yield of EBUS-TBLB in pulmonary tuberculosis was not affected by patient's age, lesion extent size, EBUS probe position, presence or tracheal grade, or characteristics of lesions (all p > .05). Conclusion:Transbronchial radial ultrasound-guided lung biopsy of tuberculous PPLs possesses higher diagnostic rate, fewer complications and less interference, exerts potential application value in the diagnosis of tuberculous peripheral pulmonary lesions.

show abstract

Section: Discussionmentioning

confidence: 99%

Diagnostic value of ultrasound‐guided transbronchial lung biopsy in peripheral tuberculous pulmonary lesions

Zou

Zhu

et al. 2022

Diagnostic Cytopathology

View full text Add to dashboard Cite

show abstract

“…For all malignant cases, it was deemed essential to consider histopathology and immunohistochemistry before starting definitive treatment. Similarly, although a combination of ROSE and molecular techniques can provide a same‐day diagnosis with a high degree of accuracy in patients with tuberculosis, 13 for cases diagnosed as having a granulomatous pathology, ancillary investigations like AFB staining, biopsy, angiotensin converting enzyme (ACE) levels, and MGIT culture were required to come to a definitive diagnosis before starting the treatment. It is also possible that granulomas may co‐exist with malignancy, as is sometimes seen in germ‐cell tumors and lymphomas 14 .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic utility of rapid on‐site evaluation of endobronchial ultrasound‐guided transbronchial needle aspiration samples: A study in a region of high tuberculosis burden

et al. 2021

Self Cite

View full text Add to dashboard Cite

Introduction Endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) has emerged as a very useful tool for precise diagnosis of thoracic lesions. However, the procedure sometimes has a poor yield. Rapid on‐site evaluation (ROSE) in EBUS‐TBNA has been shown to be helpful to improve its diagnostic yield and minimise the need for repeat procedures. Most of the studies that have evaluated the utility of ROSE have focused on malignant lesions; however, it is important to understand its utility in benign lesions, particularly in a country with a high tuberculosis burden. Objective This study was undertaken to evaluate the utility of ROSE in EBUS‐TBNA for the diagnosis of patients presenting with mediastinal lymphadenopathy and/or thoracic mass for benign and malignant pathologies. Methods This was a prospective study including 47 patients who underwent EBUS‐TBNA for thoracic lesions (lung masses and hilar/mediastinal lymphadenopathy) over a 10‐month period at a single tertiary care centre. In addition to ROSE and definitive cytology, the aspirated material was also sent for cell block and microbiological studies. Endobronchial/transthoracic biopsy was conducted for histopathological evaluation (HPE) where required, at the discretion of the clinician. ROSE diagnosis was then compared with the final diagnosis. Results Of the 47 patients examined using ROSE, granulomas were observed in 33 cases, non‐specific inflammation in six cases, and malignancy in five cases; one case was inconclusive and the sample was found to be inadequate in two cases. The ROSE assessment matched the final diagnosis in 84.45% patients. Conclusion ROSE is a simple procedure that is able to provide an immediate and accurate assessment of adequacy and has a significant yield, enabling a preliminary diagnosis to be made in both benign and malignant samples.

show abstract

Evaluation and comparison of molecular and conventional diagnostic modalities for detecting pulmonary tuberculosis in bronchoalveolar lavage fluid

Cited by 2 publications

References 16 publications

Diagnostic value of ultrasound‐guided transbronchial lung biopsy in peripheral tuberculous pulmonary lesions

Diagnostic value of ultrasound‐guided transbronchial lung biopsy in peripheral tuberculous pulmonary lesions

Diagnostic utility of rapid on‐site evaluation of endobronchial ultrasound‐guided transbronchial needle aspiration samples: A study in a region of high tuberculosis burden

Contact Info

Product

Resources

About