Evaluation and comparison of automated hematology analyzer, flow cytometry, and digital morphology analyzer for monocyte counting

Buoro, Sabrina; Moioli, Valentina; Seghezzi, Michela; Previtali, Giulia; Alessio, Mg; Lopez, Ramon-Simon; Ortolani, Claudio; Ottomano, Cosimo; Lippi, Giuseppe

doi:10.1111/ijlh.12868

Cited by 10 publications

(6 citation statements)

References 24 publications

(38 reference statements)

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“…In fact, this feature has been revealed in previous studies, which describe low correlation between basophil count of different hematology analyzers as well as high imprecision values 13 . Some publications have pointed out low agreement for monocytes in relation to microscopic assessment 14‐17 but this population correlated well in our study ( R = 0.876).…”

Section: Discussionsupporting

confidence: 70%

Performance evaluation of the new hematology analyzer UniCel DxH 900

Martínez-Iribarren

Tejedor

Sanjaume

et al. 2021

Int J Lab Hematology

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Introduction UniCel DxH900 (Beckman Coulter, Miami, Florida, USA) is a quantitative, multi‐parameter, automated hematology analyzer for in vitro diagnostic use in clinical laboratories. The aim of this study was to evaluate the analytical performance of the new DxH900 analyzer to verify its diagnostic and clinical utility in the hematology laboratory of a tertiary care hospital in Spain. The most important and novel feature offered by DxH900 analyzer is providing MDW (monocyte distribution width), a new hematologic parameter which is being clinically validated as an early sepsis indicator with promising results. Methods We evaluated imprecision (including MDW), linearity, and carryover of DxH900. Method comparison for cell blood count (CBC) was performed in relation to DxH800 with 100 samples. We compared leukocyte differential (DIFF) from DxH900 with manual 400‐cell differential. 390 samples were assessed for flag performance. Results Results obtained for between days and within‐run imprecision were good. DxH900 showed excellent linearity (R = 1.00) over analytical range for white blood cells, red blood cells, hemoglobin, platelets, and reticulocyte count (RET) (R = 0.96) and no significant carryover effect. CBC and RET on the DxH900 correlated well with DxH800 (R ≥ 0.99). Comparison with manual differential showed excellent correlation (R ≥ 0.88), except for basophils. Flagging performance exhibited sensitivity over 90% for majority of alarm messages and very high negative predictive value (over 95%). Conclusion UniCel DxH900 Coulter analyzer provides reliable results and fully comparable to DxH800. DxH900 is an accurate, highly precise analyzer with good analytical performances to be used effectively in high‐volume laboratories.

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Section: Discussionsupporting

confidence: 70%

Performance evaluation of the new hematology analyzer UniCel DxH 900

Martínez-Iribarren

Tejedor

Sanjaume

et al. 2021

Int J Lab Hematology

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“…For comparison of WBC differential counts, the Alinity hq showed comparable results to those of the Sysmex XN‐9000, and the correlation coefficients values were > 0.97 for the majority of WBC types except for monocytes (γ = 0.849) and basophils (γ = 0.618) (Table ). Lower inter‐instrument agreement for monocytes and basophils is not uncommon and literature review reports 0.56‐0.95 and 0.01‐0.82 of γ values, respectively, among different analyzers. Even the most recently introduced systems using a new slide‐based technology showed lower correlations of monocytes and basophils than other WBC subtypes on inter‐instrument comparison.…”

Section: Discussionmentioning

confidence: 99%

Performance evaluation of new Abbott Alinity hq hematology analyzer

Woo

Jeong

et al. 2019

Int J Lab Hematology

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Introduction Abbott Alinity hq is a next‐generation automated hematology analyzer providing complete blood count (CBC) with 6‐part white blood cells (WBC) differential counts. The purpose of this study was to evaluate the performance of the analyzer to verify the diagnostic and clinical utility of the Abbott Alinity hq automated system. Methods We evaluated specimen stability, precision, linearity, carry‐over, and method comparison to assess the performance of Alinity hq. For comparison of the Alinity hq with Sysmex XN‐9000, totally 314 samples from adult and pediatric patients including both normal and abnormal hematology profiles were analyzed in parallel. The Alinity hq was also compared with the manual differential counts for the same 314 samples. Results At 4°C, the Alinity hq analyzer showed no significant changes in CBC and WBC differential count up to 48 hours. When stored at room temperature (18‐25°C), all parameters except the mean platelet volume (MPV) were stable up to 36 hours. The Abbott Alinity hq analyzer demonstrated excellent reproducibility and between‐batch precision for all CBC and WBC differential parameters. WBC, red blood cells (RBC), hemoglobin (HGB), and platelets showed good linearity and acceptable carry‐over. Comparison with a Sysmex XN‐9000 analyzer and manual 400‐cell differential showed excellent correlation for CBC and WBC differential count parameters (correlation coefficient = 0.815‐0.999) except for mean corpuscular hemoglobin concentration (MCHC) and basophils. Conclusion We performed initial validation studies and confirmed performance specifications on specimen stability, precision, linearity, carry‐over, and method comparison. The Abbott Alinity hq analyzer showed good analytical performance for all standard CBC parameters.

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“…Instrumentation was Sysmex XN-9000 (Sysmex, Kobe, Japan) equipped with The Sysmex DI-60 system (DI-60; Sysmex)—an automated digital cell imaging analyzer. 23 May-Grunwald-Giemsa staining was used.…”

Section: Methodsmentioning

confidence: 99%

Platelets Promote Thromboinflammation in SARS-CoV-2 Pneumonia

Taus

Salvagno

Canè

et al. 2020

ATVB

153

182

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Objective: Pulmonary thrombosis is observed in severe acute respiratory syndrome coronavirus 2 pneumonia. Aim was to investigate whether subpopulations of platelets were programmed to procoagulant and inflammatory activities in coronavirus disease 2019 (COVID-19) patients with pneumonia, without comorbidities predisposing to thromboembolism. Approach and Results: Overall, 37 patients and 28 healthy subjects were studied. Platelet-leukocyte aggregates, platelet-derived microvesicles, the expression of P-selectin, and active fibrinogen receptor on platelets were quantified by flow cytometry. The profile of 45 cytokines, chemokines, and growth factors released by platelets was defined by immunoassay. The contribution of platelets to coagulation factor activity was selectively measured. Numerous platelet-monocyte (mean±SE, 67.9±4.9%, n=17 versus 19.4±3.0%, n=22; P <0.0001) and platelet-granulocyte conjugates (34.2±4.04% versus 8.6±0.7%; P <0.0001) were detected in patients. Resting patient platelets had similar levels of P-selectin (10.9±2.6%, n=12) to collagen-activated control platelets (8.7±1.5%), which was not further increased by collagen activation on patient platelets (12.4±2.5%, P =nonsignificant). The agonist-stimulated expression of the active fibrinogen receptor was reduced by 60% in patients ( P <0.0001 versus controls). Cytokines (IL [interleukin]-1α, IL-1β, IL-1RA, IL-4, IL-10, IL-13, IL, 17, IL-27, IFN [interferon]-α, and IFN-γ), chemokines (MCP-1/CCL2), and growth factors (VEGF [vascular endothelial growth factor]-A/D) were released in significantly larger amounts upon stimulation of COVID-19 platelets. Platelets contributed to increased fibrinogen, VWF (von Willebrand factor), and factor XII in COVID-19 patients. Patients (28.5±0.7 s, n=32), unlike controls (31.6±0.5 s, n=28; P <0.001), showed accelerated factor XII–dependent coagulation. Conclusions: Platelets in COVID-19 pneumonia are primed to spread proinflammatory and procoagulant activities in systemic circulation.

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Evaluation and comparison of automated hematology analyzer, flow cytometry, and digital morphology analyzer for monocyte counting

Cited by 10 publications

References 24 publications

Performance evaluation of the new hematology analyzer UniCel DxH 900

Performance evaluation of the new hematology analyzer UniCel DxH 900

Performance evaluation of new Abbott Alinity hq hematology analyzer

Platelets Promote Thromboinflammation in SARS-CoV-2 Pneumonia

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