Evaluation and characterization of thyroid-disrupting activities in soil samples along the Second Songhua River, China

Abstract

a b s t r a c tIn this study, a recombinant thyroid receptor (TR) gene yeast assay combined with Monte Carlo simulation were used to evaluate and characterize soil samples collected from Jilin (China) along the Second Songhua River, for their ant/agonist effect on TR. No TR agonistic activity was found in soils, but many soil samples exhibited TR antagonistic activities, and the bioassay-derived amiodarone hydrochloride equivalents, which was calculated based on Monte Carlo simulation, ranged from not detected (N.D.) to 35.5 μg/g. Hydrophilic substance fractions were determined to be the contributors to TR antagonistic activity in these soil samples. Our results indicate that the novel calculation method is effective for the quantification and characterization of TR antagonists in soil samples, and these data could provide useful information for future management and remediation efforts for contaminated soils.

Thyroid-Disrupting Activities of Groundwater from a Riverbank Filtration System in Wuchang City, China: Seasonal Distribution and Human Health Risk Assessment

“…e recombined TR gene yeast assays, including agonistic and antagonistic activity tests, were conducted as described in the previous report [9,29]. We followed the methods of Kong et al [29]. T3 and AH were selected as the positive controls for agonistic activity and antagonistic activity against TR, respectively.…”

“…In order to ensure that the obtained results from bioassays were caused by real agonistic/antagonistic responses rather than cytotoxicity, viability was also measured in cells exposed to water samples at the maximum assay concentration. We followed the methods of Kong et al [29]. Yeast cells were plated similarly to that in the original assay and exposed for 2 h to the medium in the presence of water samples.…”