Evaluation and characterization of the cellulolytic bacterium, Bacillus pumilus SL8 isolated from the gut of oriental leafworm Spodoptera litura: An assessment of its potential value for lignocellulose bioconversion

Dar, Mudasir A.; Syed, Rukhsana; Pawar, Kiran D.; Dhole, Neeraja P.; Xie, Rongrong; Pandit, Radhakrishna S.; Sun, Jianzhong

doi:10.1016/j.eti.2022.102459

Cited by 21 publications

(12 citation statements)

References 62 publications

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“…The changes in the functional groups of the COC samples before and after hydrolysis were analyzed with FTIR (Nicolet iS50, Thermo Fisher Scientific, USA). The untreated and treated COC powder samples were treated with potassium bromide (KBr) particles for the FTIR analysis ( Dar et al, 2022 ). Briefly, 6 mg of dried COC sample and 600 mg of KBr were fully ground in an agate mortar and pressed under 200 kg cm –2 for 2 min for tableting.…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, concentrated acids or bases are highly oxidative and corrosive, and are difficult to neutralize in the subsequent reactions, which leads to damage of the reaction vessels. Physical and chemical methods are effective; however, their high cost and the associated environmental issues are unsustainable ( Dar et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Cellulase, the core enzyme for depolymerizing lignocellulosic polysaccharides, breaks down the β-1,4-bonds in cellulose polymers to release glucose units ( de Paula et al, 2019 ). Cellulases are key biocatalysts in the degradation of lignocellulosic biomass for industrial applications, beginning with the animal feed industry in the early 1980s to the biorefining and biofuel industries today ( Dadwal et al, 2020 ; Dar et al, 2022 ). The conversion of lignocellulose to biofuels through cellulases to address fossil fuel depletion and greenhouse gas emissions is currently a global concern ( Melendez et al, 2022 ; Sohail et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…The conversion of lignocellulose to biofuels through cellulases to address fossil fuel depletion and greenhouse gas emissions is currently a global concern ( Melendez et al, 2022 ; Sohail et al, 2022 ). Cellulases are key biocatalysts in the degradation of lignocellulosic biomass for industrial applications, beginning with the animal feed industry in the early 1980s to the biorefining and biofuel industries today ( Dadwal et al, 2020 ; Dar et al, 2022 ). There is a growing demand for cost-effective cellulases in the biofuel industry; however, the production process of cellulases is very expensive, accounting for 50% of the total hydrolysis costs.…”

Section: Introductionmentioning

confidence: 99%

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Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Liu

Zhong

et al. 2022

Front. Microbiol.

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Coconut oil cake (COC), a byproduct of oil extraction, contains high levels of cellulose. The aim of this study was to isolate a cellulose-degrading yeast from rotten dahlia that can effectively use COC as the only carbon source for cellulase secretion. Based on screening, Meyerozyma guillermondii CBS 2030 (M. guillermondii) was identified as a potential candidate, with the highest cellulolytic activity among the yeast strains isolated, with the carboxymethyl cellulase (CMCase) activity reaching 102.96 U/mL on day 5. The cellulose in COC samples was evaluated before and after degradation by M. guillermondii. Analysis based on field emission scanning electron microscopy (FESEM) revealed that the COC structure was changed significantly during the treatment, indicating effective hydrolysis. Fourier transform infrared spectroscopy (FTIR) of the modified functional groups indicated successful depolymerization of coconut cake. X-ray diffraction (XRD) and analysis of color differences established effective degradation of COC by M. guillermondii. The results demonstrate that M. guillermondii effectively secretes CMCase and degrades cellulose, which has important practical significance in COC degradation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Liu

Zhong

et al. 2022

Front. Microbiol.

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“…Due to serious environmental issues and global climate change, researchers all over the world are trying their best to convert the fossil fuel-based society into a bio-economical society, advancing the goal of reaching peak carbon and realizing carbon neutrality [ 1 , 2 ]. Although fossil fuels play a critical role in social industrialization, these non-renewable and unsustainable fuels have negative effects on the environment and humans [ 3 , 4 ]. Lignocellulose, such as forest residues (branches, leaves, etc.…”

Section: Introductionmentioning

confidence: 99%

Peroxyacetic Acid Pretreatment: A Potentially Promising Strategy towards Lignocellulose Biorefinery

Chen

et al. 2022

Molecules

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The stubborn and complex structure of lignocellulose hinders the valorization of each component of cellulose, hemicellulose, and lignin in the biorefinery industries. Therefore, efficient pretreatment is an essential and prerequisite step for lignocellulose biorefinery. Recently, a considerable number of studies have focused on peroxyacetic acid (PAA) pretreatment in lignocellulose fractionation and some breakthroughs have been achieved in recent decades. In this article, we aim to highlight the challenges of PAA pretreatment and propose a roadmap towards lignocellulose fractionation by PAA for future research. As a novel promising pretreatment method towards lignocellulosic fractionation, PAA is a strong oxidizing agent that can selectively remove lignin and hemicellulose from lignocellulose, retaining intact cellulose for downstream upgrading. PAA in lignocellulose pretreatment can be divided into commercial PAA, chemical activation PAA, and enzymatic in-situ generation of PAA. Each PAA for lignocellulose fractionation shows its own advantages and disadvantages. To meet the theme of green chemistry, enzymatic in-situ generation of PAA has aroused a great deal of enthusiasm in lignocellulose fractionation. Furthermore, mass balance and techno-economic analyses are discussed in order to evaluate the feasibility of PAA pretreatment in lignocellulose fractionation. Ultimately, some perspectives and opportunities are proposed to address the existing limitations in PAA pretreatment towards biomass biorefinery valorization. In summary, from the views of green chemistry, enzymatic in-situ generation of PAA will become a cutting-edge topic research in the lignocellulose fractionation in future.

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A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

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Evaluation and characterization of the cellulolytic bacterium, Bacillus pumilus SL8 isolated from the gut of oriental leafworm Spodoptera litura: An assessment of its potential value for lignocellulose bioconversion

Cited by 21 publications

References 62 publications

Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Screening of cellulose-degrading yeast and evaluation of its potential for degradation of coconut oil cake

Peroxyacetic Acid Pretreatment: A Potentially Promising Strategy towards Lignocellulose Biorefinery

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

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