Evaluation and characterization of the immunomodulatory activity of the protein extract from<i>Citrullus colocynthis</i>L.

Daoudi, Abdeljlil; Bousta, Dalila; Aarab, Lotfi; Abdel‐Sattar, Essam

doi:10.1080/09540105.2011.641168

Cited by 2 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, when we cross-examined the molecular weight of the proteins obtained from the ripen fruit of C. colocynthis through SDS-PAGE, the results revealed small-sized peptides ranging from ~70 and ~55 kDa, ~40 to ~35 kDa, and ~15 to ~25 kDa in crude, 70%, and 50% fractions. It was shown that different proteins were extracted from the seeds of the plant on the basis of MW and showed sizes of 150, 67, and 36 kDa [ 17 ]. Hence, we can conclude from the previous and current study that there are dynamic proteins present in the whole and different parts of the fruit of C. colocynthis .…”

Section: Resultsmentioning

confidence: 99%

Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities

et al. 2023

View full text Add to dashboard Cite

Herbal products are preferable to synthetic medicines, and the use of traditional medicines is increasing day-by-day. The current study was designed to evaluate the potentials of bioactive compounds from Citrullus colocynthis by performing FTIR, HPLC, and GC-MS analyses, which explore the good concentration of the secondary metabolites, such as gallic acid (74.854 ppm), vanillic acid (122.616 ppm), and ferulic acid (101.045 ppm) with considerable bioactivities. Antimicrobial protein was estimated by performing SDS-PAGE, ranging from 15 to 70 kDa in all protein fractions. The current study also checked the cytotoxicity of the bioactive compounds in the active fraction of C. colocynthis, and to perform this activity, the groups of rats were arranged with 16 rats randomly divided into four groups (three experimental and one control) by administering various dosage of methanolic fractions in dose-dependent manner. Histopathology was conducted on the livers of the rats after 15 days of sacrifice under deep anesthesia. In liver cell slides examined at the maximum dose of 600 mg/kg, minimal morphological changes, such as slight ballooning, nuclear variation, vacuolar degeneration, and hydropic degeneration, were observed. Furthermore, the in silico analysis identified bioactive compounds as potential drug candidates.

show abstract

Section: Resultsmentioning

confidence: 99%

Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Spleen and Thymus were removed aseptically from animals and cell suspensions were prepared by pressing the organs through a fine wire mesh as described in previous studies. 14,8,9 These cells were washed by RPMI (Sigma-Aldrich, USA) and the red blood cells were lysed by 154 mM Ammonium Chloride. The number of viable cells was determined microscopically by trypan blue 0.1% exclusion test.…”

Section: Cell Culturementioning

confidence: 99%

“…Cell proliferation was measured by the MTT assay according to Mosmann et al, 1983 23 and as described previously. 8,9,14 In short, cells were plated at 150,000 cells/well in 96 well plates (Citotest Labware Manufacturing CO., LTD, Jiangsu, China), then incubated at 37 °C in a humidified chamber under an atmosphere of 95% air and 5 % CO2 for 72 hours. The extracts diluted in RPMI were added to cells at 1mg/ml and 2mg/ml before their incubation.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

See 1 more Smart Citation

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Laaraj

Ouahidi

Aarab

2023

EJI

View full text Add to dashboard Cite

Immunomodulators from natural products are widely used for the treatment of infectious diseases, allergies, and cancer in traditional Moroccan medicine. The purpose of this study was the study of two plant seeds of Brassica rapa (Turnip) and Raphanus sativus (Radish) used by Moroccans in traditional medicine to enhance immunity. We have prepared three different extracts from seeds using ethanol, Ethyl Acetate, or water. Immunomodulatory effects of these two plant seeds were tested on rabbit immunity cell proliferation (splenocytes, thymocytes, and macrophages) and their functions (IgG production, cytotoxicity, and phagocytosis). The results obtained indicated that only aqueous extract of B. rapa seeds revealed an immunostimulant effect on both splenocyte and thymocyte proliferation with an increase in cytotoxicity of thymocytes (MLR assay). With R. sativus seeds, we observed an important stimulation of thymocyte proliferation and their cytotoxicity under aqueous extract without effect on splenocyte or macrophages. We concluded that aqueous extract of both seeds (B. rapa and R. sativus) possessed immunostimulant properties leading to stimulation of cellular immunity responsible for defense against viruses.

show abstract

Evaluation and characterization of the immunomodulatory activity of the protein extract fromCitrullus colocynthisL.

Cited by 2 publications

References 21 publications

Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities

Characterization of Bioactive Compounds and Novel Proteins Derived from Promising Source Citrullus colocynthis along with In-Vitro and In-Vivo Activities

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Contact Info

Product

Resources

About