Evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere

Abstract

Microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. In our study, a high level of siderophore production was observed from a newly isolated Pseudomonas sp. from the rhizosphere of Chickpea plants. Under an iron depleted condition in Standard Succinic acid medium a 1000 μgmL-1 of siderophore production was achieved. Increasing the concentration of iron showed an inverse relationship between growth and siderophore production. Fourier Transform Infrared Spectroscopy (FTIR) analysis of the purified crystals, its UV spectral analysis and High Pressure Liquid Chromatography (HPLC) revealed the identity of the siderophore as similar to that of pyoverdin with distinctive characters. Electron spray ionization mass spectroscopy (ESIMS) shows presence of abundance of A1 ions (419 m/z) and branching of amino acids from B1-B5. This pyoverdin contains a cyclic tetra peptide but Serine and Arginine are missing. Based on our analysis and deviations from the reported structure of pyoverdin it is suggested that this pseudomonas produces distinctly characterized pyoverdin siderophore.

Culturable endophytic bacteria ofCamelliaspecies endowed with plant growth promoting characteristics

“…MS media and CAS reagent in a ratio of 1 : 1 were used as reference. The percentage of siderophore units produced was calculated using the formula below (Tank et al ):where, A = absorbance of CAS reagent and a = absorbance of sample at 630 nm.…”

The Chemistry of Plant–Microbe Interactions in the Rhizosphere and the Potential for Metabolomics to Reveal Signaling Related to Defense Priming and Induced Systemic Resistance

“…The common iron-binding substances in these compounds include hydroxamic acid, hydrocarboxylic acid, and catechols, as well as other related structures ( Pattus and Abdallah, 2000 ; Butler and Theisen, 2010 ; Ahmed and Holmström, 2014 ). Siderophore production is beneficial to plants (directly supply iron to plants) and is implicated in soil-borne disease suppression (reducing competitiveness of soil-borne pathogens) ( Tank et al, 2012 ). A mutant of P. putita over-expressing siderophores was more effective against Fusarium wilt in tomato when compared to a siderophore-deficient mutant of P. aeruginosa which lost its biocontrol ability.…”

Assessment of ecological diversity of rhizobacterial communities in vermicompost and analysis of their potential to improve plant growth

“…In vitro phosphate solubilization activity has been documented for many PGPR strains isolated from rhizospheric soil (Singh et al 2010;Muleta et al 2013). Siderophores are iron chelators synthesized by several PGPR and they play a vital role by directly supplying iron for plant utilization and by removing iron from the environment for the growth of phytopathogens, thereby reducing their competitiveness (Tank et al 2012). We found several siderophore producing strains belonging to pseudomonads and bacilli.…”