Evaluation and analytical applicability of a novel volumetric absorptive microsampling strategy for adherence monitoring of antihypertensive drugs by means of LC-HRMS/MS

Jacobs, Cathy M.; Kunz, Michael; Mahfoud, Felix; Wagmann, Lea; Meyer, Markus R.

doi:10.1016/j.aca.2021.339137

Cited by 8 publications

(13 citation statements)

References 39 publications

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“…The assessment of adherence based on blood, serum, or plasma drug concentrations is expected to be more accurate, particularly if a DRC approach is used [12,38,42]. Therefore, this study aimed to close an analytical gap to allow assessing adherence of the most frequently prescribed classes of AHD using just one VAMS tip [6,[27][28][29]. Parent compounds or active metabolites were used for quantification.…”

Section: Resultsmentioning

confidence: 99%

“…The volume of spiked solution did not exceed 5% of the total matrix volume [30]. To prepare calibrator and QC samples, 10 μL working solution were added to 190 μL blank human whole EDTAstabilized blood or plasma and incubated for 30 min at 37 °C and 1500 rpm using a Thermoshaker Pro (CellMedia, Elsteraue, Germany) to allow plasma-protein binding and/or diffusion into red blood cells [27]. VAMS tips were loaded by holding them onto the surface of whole blood until completely soaked with an additional waiting time of 2 s [21].…”

Section: Calibrators Quality Controls Internal Standards and Preparat...mentioning

confidence: 99%

“…Sample preparation was performed according to a previous study [27]. Briefly, dried VAMS tips were stripped into amber 2 mL reaction tubes and 90 μL purified water followed by 10 μL IS solution were added.…”

Section: Sample Preparation -Vamsmentioning

confidence: 99%

“…S1). VAMS were already used for e.g., screening procedures in different matrices, metabolic studies, therapeutic drug monitoring, and adherence monitoring [23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

“…This study aimed to close an analytical gap by improving a VAMS-based strategy [27] to allow assessing adherence to all classes of AHD listed in the current guidelines for hypertension treatment which include thiazide diuretics, calcium-channel blockers (CCBs), angiotensin-convertingenzyme (ACE) inhibitors, or angiotensin II receptor blockers (ARBs), and spironolactone in case of resistant hypertension [6,28,29]. The extraction of the VAMS tip and the analytical setup should be conserved but chromatographic conditions and mass spectrometry settings must be adapted and a proof of concept study had to be provided, including the comparison of FPB concentrations to matching plasma samples.…”

Section: Introductionmentioning

confidence: 99%

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Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

et al. 2022

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Volumetric absorptive microsampling (VAMS), an emerging microsampling technique, is a promising tool for adherence monitoring. This study focused on development of an analytical methodology to improve VAMS-based strategies for adherence assessment by analyzing angiotensin-converting-enzyme (ACE) inhibitors, loop diuretics, a potassium-sparing diuretic, and a thiazide diuretic. Development included sample preparation, chromatographic conditions, mass spectrometry settings, validation, and demonstrating proof of concept. Quantification of analytes, by name furosemide, hydrochlorothiazide, lisinopril, torasemide, and the active metabolites, canrenone, enalaprilat, and ramiprilat in finger prick blood (FPB), was validated based on international guidelines. Selectivity, carryover, and within/between-run accuracy and precision were in accordance with the recommendations. The matrix effect was evaluated at three different hematocrit levels (HT: 20%, 40%, 60%) and the coefficients of variation did not exceed 15%. Dilution integrity (1:10 and 1:20) was given for all analytes except lisinopril, yet for lisinopril, the therapeutic range was already covered by the calibration range. Long-term stability in VAMS tips was tested for 2 weeks at 24 °C in the dark and revealed no degradation of analytes. The proof of concept was performed by analyzing 35 intakes of ACE-inhibitors and diuretics in 18 VAMS and matched plasma samples. Hereby, determined concentration in FPB and plasma cannot be used interchangeably, and thus specific reference ranges for whole blood must be established. Nevertheless, the VAMS-based strategy was shown to be suitable for assessing adherence of all classes of antihypertensive drugs used in the guidelines to manage hypertension. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Section: Calibrators Quality Controls Internal Standards and Preparat...mentioning

confidence: 99%

Section: Sample Preparation -Vamsmentioning

confidence: 99%

“…S1). VAMS were already used for e.g., screening procedures in different matrices, metabolic studies, therapeutic drug monitoring, and adherence monitoring [23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

et al. 2022

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Towards clinical adherence monitoring of oral endocrine breast cancer therapies by LC-HRMS—method development, validation, comparison of four sample matrices, and proof of concept

Jacobs,

Radosa,

Wagmann

et al. 2024

Anal Bioanal Chem

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Oral endocrine therapies (OET) for breast cancer treatment need to be taken over a long period of time and are associated with considerable side effects. Therefore, adherence to OET is an important issue and of high clinical significance for breast cancer patients’ caregivers. We hypothesized that a new bioanalytical strategy based on liquid chromatography and high-resolution mass spectrometry might be suitable for unbiased adherence monitoring (AM) of OET. Four different biomatrices (plasma, urine, finger prick blood by volumetric absorptive microsampling (VAMS), oral fluid (OF)) were evaluated regarding their suitability for AM of the OET abemaciclib, anastrozole, exemestane, letrozole, palbociclib, ribociclib, tamoxifen, and endoxifen. An analytical method was developed and validated according to international recommendations. The analytical procedures were successfully validated in all sample matrices for most analytes, even meeting requirements for therapeutic drug monitoring. Chromatographic separation of analytes was achieved in less than 10 min and limits of quantification ranged from 1 to 1000 ng/mL. The analysis of 25 matching patient samples showed that AM of OET is possible using all four matrices with the exception of, e.g., letrozole and exemestane in OF. We were able to show that unbiased bioanalytical AM of OET was possible using different biomatrices with distinct restrictions. Sample collection of VAMS was difficult in most cases due to circulatory restraints and peripheral neuropathy in fingers and OF sampling was hampered by dry mouth syndrome in some cases. Although parent compounds could be detected in most of the urine samples, metabolites should be included when analyzing urine or OF. Plasma is currently the most suitable matrix due to available reference concentrations. Graphical Abstract

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Image classification combined with faster R–CNN for the peak detection of complex components and their metabolites in untargeted LC-HRMS data

Zeng

2023

Analytica Chimica Acta

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Evaluation and analytical applicability of a novel volumetric absorptive microsampling strategy for adherence monitoring of antihypertensive drugs by means of LC-HRMS/MS

Cited by 8 publications

References 39 publications

Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

Closing the gap — development of an analytical methodology using volumetric absorptive microsampling of finger prick blood followed by LC-HRMS/MS for adherence monitoring of antihypertensive drugs

Towards clinical adherence monitoring of oral endocrine breast cancer therapies by LC-HRMS—method development, validation, comparison of four sample matrices, and proof of concept

Image classification combined with faster R–CNN for the peak detection of complex components and their metabolites in untargeted LC-HRMS data

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